The aim of our research was to diminish the damaging effect of vaccination on the quality of stallion sperm and its cryostability. The task was to study the effect of the complex vitamin of tetrahydrovit on sperm parameters under conditions of vaccination against leptospirosis. Stallions that were vaccinated against leptospirosis were divided into 3 groups: the first –control, without vitamin supplements, the second – with the addition of 2 ml of tetrahydrovit vitamin daily, the third group – intramuscular injection of tetrahydrovit 1 time in 7 days. The reaction of the body to the introduction of the vaccine in the group without vitamins proceeded with a significant decrease in the quality of sperm in the first two weeks. Later, the motility and the number of whole membranes of fresh sperm gradually improved, but the survival rate of chilled and frozen sperm remained low until the end of the observations. Among stallions in the experimental groups, which were given tetrahydrovit vitamin (orally or intramuscularly), the decrease in sperm parameters was insignificant, within the usual fluctuations, which allowed one to avoid culling spermodoses. Tetrahydrovit vitamin promotes a stabilizing effect on the quality indicators of sperm of stallions-producers after vaccination.