Influence of compressed fluids treatment on the activity of Yarrowia lipolytica lipase

Oliveira, Débora de; Feihrmann, Andresa Carla; Dariva, Cláudio; Cunha, Aline G.; Bevilaqua, Juliana V.; Destain, Jean‐Pierre; Oliveira, J. Vladimir; Freire, Denise Maria Guimarães

doi:10.1016/j.molcatb.2006.01.016

Cited by 71 publications

(40 citation statements)

References 35 publications

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“…We can conclude that the exposure of enzymes to SC-CO 2 in a continuous-flow reactor has a positive effect on the activity of the examined lipases, which is in agreement with the recent experimental results published by Giessauf and Gamse [9], Bauer et al [10,11], and Yan et al [12], but opposite to those reported by Oliveira et al [19].…”

Section: Effect Of Lipozymesupporting

confidence: 91%

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Lipase Activity Enhancement by SC-CO₂ Treatment

2008

Zeitschrift Für Naturforschung B

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The activity of lipases from porcine pancreas, Candida antartica recombinant from Aspergillus oryzae, Candida cylindracea (immobilized), Penicilium roqueforti, Aspergillus niger, Rhizopus arrhizus, Mucor miehei (two types of immobilization), and Pseudomonas cepacia (two types of immobilization) was studied after using them as biocatalysts of blackcurrant oil hydrolysis under SC-CO 2 conditions. The reaction was performed at 40 • C and 15 MPa in a continuous-flow reactor. Increased relative activity of all used lipases after the hydrolytic reaction was observed. The most remarkable increase in the activity was noted for the lipase from Rhizopus arrhizus which was increased by more than 50 times. The highest activity was shown by Lipozyme R , lipase from Mucor miehei immobilized on macroporous resin. Both treated and untreated Lipozyme R were used as biocatalysts in hydrolytic resolution of the racemic cis-or trans-isomers of 2-(4-methoxybenzyl)cyclohexyl acetates. Satisfactory reaction yields (40 %) and excellent enantiomeric purity of the products (E = 472) were obtained when hydrolysis of the trans-isomer of 2-(4-methoxybenzyl)cyclohexyl acetate was catalyzed by Lipozyme R treated with SC-CO 2 .

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Section: Effect Of Lipozymesupporting

confidence: 91%

“…The best results with excellent enantioselectivity (E = 472) were obtained when substrate 2 was hydrolyzed by Lipozyme R treated with SC-CO 2 . The spectral data of reaction products were in agreement with previously published data [18,19].…”

Section: Effect Of Lipozymesupporting

confidence: 90%

Lipase Activity Enhancement by SC-CO₂ Treatment

2008

Zeitschrift Für Naturforschung B

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“…Lipase esterification activity The esterification activity of Novozym 435 was determined as the initial rate of the esterification reaction between lauric acid and n-propanol as described by Oliveira et al (2006). All enzymatic activity determinations were performed in triplicate.…”

Section: Reuse Of Biocatalystmentioning

confidence: 99%

Lipase-catalyzed ethanolysis of Jatropha curcas L. oil assisted by ultrasonication

Lerin

Remonatto

Pereira

et al. 2017

Braz. J. Chem. Eng.

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-Transesterification of non-edible oils using immobilized lipase is a promising process for biodiesel production. Thus, this study aimed to evaluate the enzymatic transesterification of the non-edible Jatropha curcas oil for FAEE production under ultrasound irradiation in a solvent-free system. The effects of enzyme concentration, water concentration, molar ratio of ethanol to oil and ultrasound power on the FAEE conversion have been evaluated. The results show that enzyme concentration and irradiation power have a positive significant effect on FAEE conversion, where an increase in these variables leads to higher conversions. Conversion above 54% of FAEE was achieved with 1.5 hours of reaction time using ultrasound irradiation, reducing reaction time by at least 3 times, when compared with the same experimental conditions without ultrasound irradiation. Results showed that ultrasound can improve reaction conversion mainly by enhancing the mass transfer between the constituents of the reactions.

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“…Then, the lauric acid consumption was determined. One unit of activity (U) was defined as the amount of enzyme necessary to consume 1μmol of lauric acid per minute (Oliveira et al, 2006). All enzymatic activity determinations were performed in at least three replicates.…”

Section: Lipase Esterification Activitymentioning

confidence: 99%

“…For example, esters produced from long-chain fatty acids (12-20 carbon atoms), as well as short-chain fatty acids (2-8 carbon atoms), are increasingly used in the food, detergent, plasticizer, lubrificant, cosmetic and pharmaceutical industries (Vedejs and Jure, 2005;Oliveira et al, 2006;Gottor, 1999).…”

Section: Introductionmentioning

confidence: 99%

Successive cycles of utilization of novozym 435 in three different reaction systems

Lerin

Ceni

Richett

et al. 2011

Braz. J. Chem. Eng.

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-The main focus of this work was to investigate the residual esterification activity and the product conversion after 10 successive cycles of utilization of a commercial lipase in three systems: esterification of 2-ethyl hexanol and palmitic acid in a solvent-free system; esterification of ascorbic acid and palmitic acid in tert-butanol; and transesterification of glycerol and methyl benzoate in 2-propanol. These systems were chosen based on previous results by our research group in terms of product conversion. Before scale-up, there is a need for evaluating several cycles of utilization of the biocatalyst. The esterification of 2-ethyl hexanol showed that after 10 cycles the enzyme retained 90% of its activity. The system consisting of ascorbic acid, palmitic acid, Novozym 435 and tert-butanol showed that a reduction in enzyme activity was accompanied by a reduction in reaction conversion; the same behavior was not observed for the third system.

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Influence of compressed fluids treatment on the activity of Yarrowia lipolytica lipase

Cited by 71 publications

References 35 publications

Lipase Activity Enhancement by SC-CO₂ Treatment

Lipase Activity Enhancement by SC-CO₂ Treatment

Lipase-catalyzed ethanolysis of Jatropha curcas L. oil assisted by ultrasonication

Successive cycles of utilization of novozym 435 in three different reaction systems

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Influence of compressed fluids treatment on the activity of Yarrowia lipolytica lipase

Cited by 71 publications

References 35 publications

Lipase Activity Enhancement by SC-CO2 Treatment

Lipase Activity Enhancement by SC-CO2 Treatment

Lipase-catalyzed ethanolysis of Jatropha curcas L. oil assisted by ultrasonication

Successive cycles of utilization of novozym 435 in three different reaction systems

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Product

Resources

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Lipase Activity Enhancement by SC-CO₂ Treatment

Lipase Activity Enhancement by SC-CO₂ Treatment