Influence of cryopreservation on structure and function of mammalian spermatozoa: an overview

Ezzati, M; Shanehbandi, Dariush; Hamdi, Kobra; Rahbar, Soulmaz; Pashaiasl, Maryam

doi:10.1007/s10561-019-09797-0

Cited by 65 publications

(56 citation statements)

References 145 publications

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“…However, spermatozoa are subjected to drastic changes in temperature, ice crystal formation and diverse types of stresses (physical, chemical, osmotic and oxidative) during the cryopreservation process, which severely compromise sperm quality and fertility [4][5][6]. In addition, differences between species in terms of sperm size, shape and lipid-protein composition denote that the cryopreservation process is not equally efficient among all species [7].…”

Section: Introductionmentioning

confidence: 99%

“…Thereby, increasing our knowledge about the molecular modifications suffered by sperm through the cryopreservation process will aid the optimization of the current freezing-thawing protocols for a better preservation of sperm functionality and fertility. In recent years, the use of different omics technologies in the field of sperm cryobiology has led, among other things, to a greater understanding of those structural and functional changes experienced by sperm during the freezing-thawing process as well as the identification of relevant freezability biomarkers [4,11,12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

147

118

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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

147

118

View full text Add to dashboard Cite

show abstract

“…During cryopreservation, cold shock and crystal formation from intra- and extra-cellular water induce cryo-injuries that disturb the sperm plasma membrane integrity and lipid composition, resulting in the leakage of intracellular contents. Consequently, sperm metabolism is reduced [ 2 ] and apoptotic-like changes occur in the cells [ 3 , 4 , 5 ]. In particular, sperm cells are more sensitive to environmental changes because of their limited protein and lipid biosynthetic abilities [ 6 ] and the absence of DNA repair mechanism [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, the commercial and homemade available freezing medium does not fully satisfy the requirements needed for the complete protection of sperm during the cryopreservation process [ 16 ]. Although Tris-egg yolk buffer is the most commonly used diluent for mammalian sperm, many studies show that the supplementation of anti-apoptotic factors, anti-oxidants, post-thaw enhancing chemicals, or novel cryo-protective agents is required to get good post-thaw results [ 1 , 5 , 17 , 18 ]. Thus, various molecules such as metformin [ 19 ], cholesterol [ 20 ], or α-tocopherol [ 21 ] have been successfully used in canine sperm cryopreservation to reduce oxidative stress, DNA damage [ 19 , 20 ], improve motility [ 21 ], and protect plasma membrane and acrosome integrity [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Mahiddine

Kim

Qamar

et al. 2020

Animals

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This study investigated the effects of conditioned medium (CM) from canine amniotic membrane-derived MSCs (cAMSCs) on dog sperm cryopreservation. For this purpose, flow cytometry analysis was performed to characterize cAMSCs. The CM prepared from cAMSCs was subjected to proteomic analysis for the identification of proteins present in the medium. Sperm samples were treated with freezing medium supplemented with 0%, 5%, 10%, and 15% of the CM, and kinetic parameters were evaluated after 4–6 h of chilling at 4 °C to select the best concentration before proceeding to cryopreservation. Quality-related parameters of frozen–thawed sperm were investigated, including motility; kinetic parameters; viability; integrity of the plasma membrane, chromatin, and acrosome; and mitochondrial activity. The results showed that 10% of the CM significantly enhanced motility, viability, mitochondrial activity, and membrane integrity (p < 0.05); however, the analysis of chromatin and acrosome integrity showed no significant differences between the treatment and control groups. Therefore, we concluded that the addition of 10% CM derived from cAMSC in the freezing medium protected dog sperm during the cryopreservation process.

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“…Sperm cryopreservation is an essential technique for fertility management (Ezzati et al, 2020). However, the post-thaw viability of sperm differs among bulls-a research paper in this collection by Ugur et al describes a multivariate and univariate analysis to identify potential freezability biomarkers.…”

Section: Sperm Contribution To the Zygote: Egg Activation Sperm Cryomentioning

confidence: 99%

Editorial: Sperm Differentiation and Spermatozoa Function: Mechanisms, Diagnostics, and Treatment

Avidor‐Reiss

Zhang

2020

Front. Cell Dev. Biol.

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Influence of cryopreservation on structure and function of mammalian spermatozoa: an overview

Cited by 65 publications

References 145 publications

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Editorial: Sperm Differentiation and Spermatozoa Function: Mechanisms, Diagnostics, and Treatment

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