Influence of culture media and environmental factors on mycelial growth and conidial production of Diplocarpon mali

Abstract

Aim: To identify media and environmental conditions suitable for rapid mycelial growth and sporulation of Diplocarpon mali. Methods and Results: Liquid shake cultures were used to evaluate effects of media and environmental conditions on mycelial growth and conidial production of D. mali. Carrot sucrose broth (CSB), potato and carrot dextrose broth (PCDB) and potato and carrot sucrose broth (PCSB) were most favourable for rapid mycelial growth. PCDB, PCSB, PCB (potato and carrot broth) and carrot dextrose broth (CDB) were favourable for conidial production. All carbon sources tested and peptone favoured for mycelial growth. Carbon and nitrogen sources tested did not significantly stimulate conidial production. The optimum temperature for mycelial growth and conidial production was 25°C. No mycelial growth occurred at 5 or 30°C, but D. mali survived at these temperatures. Active mycelial growth occurred at pH 5–7, and pH 5–8 was favourable for sporulation. Conclusions: PCDB and PCSB incubated at 25°C for 14 day are recommended for mycelial growth and conidial production of D. mali. Significance and Impact of the Study: The information generated in this study will facilitate mycological and pathological research on D. mali and Marssonina leaf blotch of apple caused by D. mali.

Marssonina Leaf Blotch on the Apple Orchard in Batu, Indonesia

“…25 o C. It is similar to Zhao et al (2010) which states that the optimum temperature for fungal growth is around 25 o C. This pattern has no difference between observation time from three to six days after inoculation; but after seven days, the optimum temperature range is wider between 20 to 30 o C (Table 2). It was suspected because of the limited nutrients with the aging of the growing cultural media.…”

“…Similar results are presented by Zhao et al (2010) show that the potato carrot dextrose broth(PCDB) and potato carrot broth sucrose (PCSB) are suitable for M. coronaria (teleomorph: Diplocarpon mali) in cases of mycelial growth and production of conidia. Meanwhile the results of Lee et al (2011) shows the highest mycelial growth is observed on mannose and bacto-peptone amended media with colonies 9.0 and 18.5 mm in diameter resulted by using the carbon and nitrogen sources.…”

Evaluation of fresh culture media for mycelial growth and conidial production of Botrytis fabae, causing chocolate spot on faba bean (Vicia faba L.)

“…In this study, culture media strongly influenced growth, sclerotial and conidial formation, and sporulation of B. fabae. Several studies also found similar results with regard to the effect of culture media on growth, sporulation and other cultural characteristics of various types of fungi (Jackson and Bothast, 1990;Kim et al, 2005;Zhao et al, 2010;Ibrahim and Shehu, 2015;Koley and Mahapatra, 2015;Mishra and Tripathi, 2015).…”

“…Fresh media preparations from chickpea, soybean, field pea and faba bean contained high concentration of C and N sources, which supported maximum mycelial growth of the pathogen. Previous research studies also indicated in their studies with various types of fungi that high concentration of C and N induces vegetative and abundant mycelial growth (Saha et al, 2008;Zhao et al, 2010;Mishra and Tripathi, 2015;Koley and Mahapatra, 2015).…”

“…It is well known that growth and sporulation are important phases during the life of fungi, which is considerably influenced by external growth factors (Mishra and Tripathi, 2015). Among the external growth factors, nutrition was one of the determinants that were previously proved by several workers in many fungal pathogens using different culture media sources (Kasar et al, 2004;Rani and Murthy, 2004;Younis et al, 2004;Kim et al, 2005;Zhao et al, 2010;Lazarotto et al, 2013).…”