Frost damage and/or cold stress to crops in the United States runs more than $1 billion per year. In North Carolina during 1983, about 30,000 t of peanuts valued at $16 million were diverted from the edible market due to freeze damage. It is difficult to distinguish if a lot of peanut seed has been exposed to cold or heat stress because in both cases anaerobic respiration predomi nates. This research was undertaken to distinguish be tween cold and heat stresses at the cellular level on peanut seed by using dynamic headspace analysis, spe cific conductivity of the seed leachate, ion chromatogra phy for cations and organic acids, ninhydrin detection for amino acids, and light microscopic techniques. Acetaldehyde, ethanol, and ethyl acetate are produced by both stress conditions. Two isomers of 2,3-butanediol and short-chain acids were identified from cold-stressed seed. These isomers were not found in heat-stressed peanut tissues and can be used to differentiate between the two environmental stresses. Specific conductivity of the leachate was higher from cold-stressed seed than from normal or heat-stressed seed, due to a higher efflux of potassium and acetic acid from the cells. Plasmolysis of the cold-stressed seed cells was consistently observed. Aleurone grains appeared to be larger, more dispersed, and tended to migrate towards the cell wall in the heattreated samples, whereas the aleurone grains in the coldstressed samples were smaller and more concentrated at the center of the cell. Irregular cell shape was common to both stresses.