Influence of Dairy Cows Bedding Material on the Microbial Structure and Antibiotic Resistance Genes of Milk

Wu, Haoming; Wang, Yang; Du, Bingyao; Li, Huiying; Dong, Lei; Hu, Haiyan; Meng, Lu; Zheng, Nan; Wang, Jiaqi

doi:10.3389/fmicb.2022.830333

Cited by 4 publications

(5 citation statements)

References 41 publications

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“…Related reagents to be used were MycoEasy rapid mycoplasma detection kit (Cellapy), TAE (50 ×) (Beyotime), Agarose (Biofroxx), DNA ladder (Beyotime), and GoodView Nucleic Acid Dye (SBS Genetech). The supernatant of cell culture will be collected, and follow the Mycoplasma Detection Kit operation manual for subsequent operations The positive standard for mycoplasma is about 280 BP, and the primer dimer was about 80 BP [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Exploring the oncogenic potential of circSOD2 in clear cell renal cell carcinoma: a novel positive feedback loop

Yao,

Fu,

Dai

et al. 2024

J Transl Med

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Background Existing studies have found that circular RNAs (circRNAs) act as sponges for micro RNAs (miRNAs) to control downstream genes. However, the specific functionalities and mechanisms of circRNAs in human clear cell renal cell carcinoma (ccRCC) have yet to be thoroughly investigated. Methods Patient cohorts from online databases were used to screen candidate circRNAs, while another cohort from our hospital was obtained for validation. CircSOD2 was identified as a potential oncogenic target, and its relevant characteristics were investigated during ccRCC progression through various assays. A positive feedback loop containing downstream miRNA and its target gene were identified using bioinformatics and validated by luciferase reporter assays, RNA pull-down, and high-throughput sequencing. Results CircSOD2 expression was elevated in tumor samples and significantly correlated with overall survival (OS) and the tumor stage of ccRCC patients, which appeared in the enhanced proliferation, invasion, and migration of tumor cells. Through competitive binding to circSOD2, miR-532-3p can promote the expression of PAX5 and the progression of ccRCC, and such regulation can be salvaged by miR-532-3p inhibitor. Conclusion A novel positive feedback loop, PAX5/circSOD2/miR-532-3p/PAX5 was identified in the study, indicating that the loop may play an important role in the diagnosis and prognostic prediction in ccRCC patients.

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Section: Methodsmentioning

confidence: 99%

Exploring the oncogenic potential of circSOD2 in clear cell renal cell carcinoma: a novel positive feedback loop

Yao,

Fu,

Dai

et al. 2024

J Transl Med

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“…Note: The positive standard for mycoplasma is about 280 BP, and the primer dimer was about 80 BP. ( Wu et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…The harvested DP T lymphocytes were transferred to lymphoid differentiation material-coated plates, and 10 ng/mL IL-2, 10 ng/mL IL-15, and 6.25 µL human CD3/CD28/CD2 T cell activator were added to each well for 7–14 days for maturation and differentiation of CD8 + T cells ( Minagawa et al, 2018 ; Hu et al, 2020 ; Ji et al, 2022 ; Wu et al, 2022 ; Jian et al, 2023 ). Except for mature T cells, most immature lymphocytes, including CD4 + CD8 + DP T cells, died as a result of T cell receptor (TCR) rearrangement.…”

Section: Methodsmentioning

confidence: 99%

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Regeneration of T cells from human-induced pluripotent stem cells for CAR-T cell medicated immunotherapy

Chen,

Huang,

Niu

et al. 2023

Front. Bioeng. Biotechnol.

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Background: Chimeric antigen receptor (CAR) T cell treatment involves in vitro production of T cells from patient blood with synthetic receptors specific to a cancer antigen. They circumvent the major histocompatibility complex to recognize the tumor antigen, reducing hematologic malignancy remission rates by 80%. Considering the efficacy of CAR-T treatment, the present work aimed at generating functional clusters of differentiation (CD)8 + T cells from human induced pluripotent stem cells (hiPSC) and to generate hiPS-CAR-T cells with high antigen-specific cytotoxicity.Methods: The Alkaline phosphatase assay and MycoEasy rapid mycoplasma detection kit was implemented for detection of hiPSCs and mycoplasma, respectively. The CD34+ HSPCs were harvested in AggreWellTM 400 using a 37-micron reversible strainer. Likewise, the lymphoid progenitor and CD4+CD8+ DP T cells were also harvested. The Cell Counting Kit-8 (CCK-8) assay was used to mark cytotoxicity and ELISA was used to detect IFN-γ secretion. Further, flow cytometry and transwell chambers were used to assess cell cycle, and migration and invasion. Finally, the in vivo antitumor effects of the CAR-T cells were evaluated using experimental animals (mice).Results: Results revealed that a serum-free, feeder layer-free differentiation system significantly yielded hiPSC-based T cell immunotherapy with interleukin-2, interleukin-15, and activators at the differentiation stage to promote the maturation of these cells into human induced pluripotent stem (hiPS)-T cells. The infection of hiPSCs with the CD19 CAR lentivirus resulted in the production of the hiPSC-CAR-T cells. We validated the function of hiPS-CAR-T cells in vivo and in vitro experimentation which revealed no significant differences in cell morphology and function between hiPSC-derived hiPS-CAR-T cells and peripheral blood-derived CAR-T cells.Conclusion: This study developed a culture method that is efficient and clinically useful to make functional CD8+ T cells from hiPSC and to get hiPS-CAR-T cells with high antigen-specific cytotoxicity that are not very different from CAR T cells found in peripheral blood. As a result, our findings may open the way for the clinical use of hiPSC to create functional CD8+ T and hiPS-CAR-T cells cells for use in cell-based cancer therapy.

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“…These suggest that HGT may play a vital role in the spread of aminoglycoside-, β-lactamasand FCA-resistant genes in camel milk samples. More and more studies have reported that HGT is an important pathway for the transmission of ARGs, causing antibiotic-resistant bacteria to emerge in a variety of environments, as well as in raw milk [42,43].…”

Section: Transmission Of Args In Camel Milkmentioning

confidence: 99%

The Antibiotic Resistome and Its Association with Bacterial Communities in Raw Camel Milk from Altay Xinjiang

Qin,

Huang,

Yang

et al. 2023

Foods

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Raw camel milk is generally contaminated with varied microbiota, including antibiotic–resistant bacteria (ARB), that can act as a potential pathway for the spread of antibiotic resistance genes (ARGs). In this study, high–throughput quantitative PCR and 16S rRNA gene–based Illumine sequencing data were used to establish a comprehensive understanding of the antibiotic resistome and its relationship with the bacterial community in Bactrian camel milk from Xinjiang. A total of 136 ARGs and up to 1.33 × 108 total ARG copies per gram were identified, which predominantly encode resistance to β–lactamas and multidrugs. The ARGs’ profiles were mainly explained by interactions between the bacteria community and physicochemical indicators (77.9%). Network analysis suggested that most ARGs exhibited co–occurrence with Corynebacterium, Leuconostoc and MGEs. Overall, raw camel milk serves as a reservoir for ARGs, which may aggravate the spread of ARGs through vertical and horizontal gene transfer in the food chain.

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Influence of Dairy Cows Bedding Material on the Microbial Structure and Antibiotic Resistance Genes of Milk

Cited by 4 publications

References 41 publications

Exploring the oncogenic potential of circSOD2 in clear cell renal cell carcinoma: a novel positive feedback loop

Exploring the oncogenic potential of circSOD2 in clear cell renal cell carcinoma: a novel positive feedback loop

Regeneration of T cells from human-induced pluripotent stem cells for CAR-T cell medicated immunotherapy

The Antibiotic Resistome and Its Association with Bacterial Communities in Raw Camel Milk from Altay Xinjiang

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