Influence of dietary arginine on sexual dimorphism of arginine metabolism in mice

Ruzafa, Carolina; Monserrat, F.; Cremades, Adolfo Díaz-Bautista; Peñafiel, Rafael

doi:10.1016/s0955-2863(03)00055-x

Cited by 13 publications

(8 citation statements)

References 38 publications

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“…Our hypothesis is also sustained by the higher expression levels of AII and OAT genes in female than male mouse kidneys. Indeed, we recently observed that the levels of OAT mRNA and protein were higher in the former than in the latter (Levillain et al, unpublished data) and OAT activity exhibited the same gender difference [31,41]. The high AII and OAT activities in mitochondria of the female mouse PST support the view that L-arginine-derived ornithine may be a preferential source of L-glutamate and L-glutamine under specific physiological conditions.…”

Section: Discussionmentioning

confidence: 64%

“…In addition, it is noteworthy that mice exhibit a marked renal sexual dimorphism because the kidneys of male Swiss mice differ from those of the female not only in size but in many cellular and molecular properties. Recently, the existence of a clear sexual dimorphism in L-arginine content and metabolism in the murine kidney has been reported [41]. L-arginine content was 1.4-and 1.7-fold higher in the plasma and kidneys respectively of female than male mice suggesting that differences in L-arginine anabolism, catabolism and/or homeostasis between the kidneys from females and males may have physiological relevance.…”

Section: Discussionmentioning

confidence: 99%

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Localization and differential expression of arginase II in the kidney of male and female mice

Levillain

Balvay

Peyrol

2004

Pflugers Arch - Eur J Physiol

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Arginase II (AII) has been almost exclusively studied in male mammalian kidneys. Our investigations were conducted to localize AII gene expression in the female mouse kidney, and to analyze the differential expression of AII gene at the transcriptional and translational levels in the kidneys of female and male mice. Total RNAs and soluble proteins extracted from renal zones and whole kidneys were analyzed by Northern and Western blots, respectively. Mitochondrial and cytosolic proteins were analyzed by Western blot. L-[guanidino-14C]arginine hydrolysis by AII was detected in microdissected tubules and the 14CO2 released from [14C]urea hydrolysis was quantified. The results of these experiments showed that: (1) both AII mRNA and protein were highly expressed in the deep cortex and the outer stripe of the outer medulla, (2) urea was produced mainly in the proximal straight tubules (PST), (3) the 38-kDa AII protein was more abundant in the mitochondria than the cytosol, and (4) the renal content of AII mRNA and protein was about three-fold higher in female than in male mice. In conclusion, in both genders, AII gene expression is restricted to the PST and localized into mitochondria. AII gene is differentially expressed in the kidney of female and male mice since higher levels of AII mRNA, protein and activity were observed in the kidneys of the former than those of the latter. Renal AII gene expression was gender-dependent in mice but not in rats. Finally, in the PST of females, L-arginine-derived ornithine may be a precursor for the renal production of L -glutamate and L-glutamine because high levels of AII, ornithine aminotransferase and glutamine synthetase are expressed in this nephron segment.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Localization and differential expression of arginase II in the kidney of male and female mice

Levillain

Balvay

Peyrol

2004

Pflugers Arch - Eur J Physiol

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“…However, it is still unknown whether testosterone downregulates directly or indirectly the renal expression of Oat. This result is probably not restricted to the Swiss OF1 strain because Oat also seems to be differentially expressed in C57BL/6 (43), Swiss CD1 (36), and, to a lesser extent, CFW mice (27). Furthermore, in an attempt to understand whether female sex hormones affect and increase Oat expression in the female mouse kidney during postnatal development, mice were ovariectomized.…”

Section: Discussionmentioning

confidence: 99%

Sex-differential expression of ornithine aminotransferase in the mouse kidney

Levillain¹,

Déchaud²,

Hobeika³

et al. 2007

American Journal of Physiology-Renal Physiology

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The mouse kidney expresses the gene of ornithine aminotransferase (Oat). Previous works suggest that Oat is differentially expressed in female and male mouse kidney (Alonso E, Rubio V. Biochem J 259: 131-138, 1989; Levillain O, Diaz JJ, Blanchard O, Dechaud H. Endocrinology 146: 950-959, 2005; Manteuffel-Cymborowska M, Chmurzynska W, Peska M, Grzelakowska-Sztabert B. Int J Biochem Cell Biol 27: 287-295, 1995; Natesan S, Reddy SR. Comp Biochem Physiol B Biochem Mol Biol 130: 585-595, 2001; Yu H, Yoo PK, Aguirre CC, Tsoa RW, Kern RM, Grody WW, Cederbaum SD, Iyer RK. J Histochem Cytochem 51: 1151-1160, 2003). This study was designed to provide a detailed description of the sexual dimorphism of Oat expression in the mouse kidney and to test the influence of sex hormones on its regulation. Experiments were performed on male and female Swiss OF1 mice during their postnatal development, at adulthood, and in orchidectomized and ovariectomized mice. Kidneys, dissected renal zones, and mitochondria were used to analyze OAT mRNA and protein levels and measure OAT activity. The results revealed that before puberty, Oat expression was similar between female and male kidneys whereas from puberty until adulthood Oat expression increased in the female kidney, becoming approximately 2.5-fold higher than in the male kidney. This sex-differential expression of Oat was associated with a sex-specific distribution of Oat along the corticopapillary axis and within the nephron. OAT was three- to fourfold more expressed in the female than the male cortex. In males, Oat was highly expressed in the medulla, mainly in the thick ascending limbs. Renal Oat distribution in orchidectomized mice resembled that in the females. Ovariectomy did not influence Oat expression. Sex differences are explained by the physiological increase in plasma testosterone in males. Expression of medium-chain acyl-CoA synthetase protein confirmed this finding. We report sexual dimorphism of Oat expression in the mouse kidney and show that Oat is naturally downregulated in the presence of testosterone.

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“…A marked sex dimorphism has been reported in the levels of the essential AA, L-Arginine (Arg) in plasma, kidney, and skeletal muscle (Ruzafa et al., 2003). Arg enters in the urea cycle, and serves as a precursor for the synthesis of a number of important biological molecules including Nitric oxide, and guanidine (Reyes et al., 1994; Wu and Morris, 1998; Wu and Meininger, 2002).…”

Section: Differences In Substrate Metabolismmentioning

confidence: 99%

“…Dietary Arg restriction induces a marked decrease of plasma and tissues Arg that almost equalizes the sexual dimorphism found in the normal levels of this AA (Morris, 2002). The interaction between dietary Arg and hormone activity is suggested by the observation that Arg dietary restriction affects the activities of enzymes related to the metabolism of Arg and ornithine that are regulated by sex hormones (Ruzafa et al., 2003). Moreover, Arg restriction induces a significant decrease of both body and renal weights in males, but not in females probably due to an interaction between Arg and the mechanism of action of testosterone.…”

Section: Differences In Substrate Metabolismmentioning

confidence: 99%

Sex Hormones and Macronutrient Metabolism

Comitato

Saba

Turrini

et al. 2014

Critical Reviews in Food Science and Nutrition

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The biological differences between males and females are determined by a different set of genes and by a different reactivity to environmental stimuli, including the diet, in general. These differences are further emphasized and driven by the exposure to a different hormone flux throughout the life.These differences have not been taken into appropriate consideration by the scientific community. Nutritional sciences are not immune from this “bias” and when nutritional needs are concerned, females are considered only when pregnant, lactating or when their hormonal profile is returning back to “normal,” i.e., to the male-like profile.The authors highlight some of the most evident differences in aspects of biology that are associated with nutrition.This review presents and describes available data addressing differences and similarities of the “reference man” vs. the “reference woman” in term of metabolic activity and nutritional needs. According to this assumption, available evidences of sex-associated differences of specific biochemical pathways involved in substrate metabolism are reported and discussed. The modulation by sexual hormones affecting glucose, amino acid and protein metabolism and the metabolization of nutritional fats and the distribution of fat depots, is considered targeting a tentative starting up background for a gender concerned nutritional science.

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Influence of dietary arginine on sexual dimorphism of arginine metabolism in mice

Cited by 13 publications

References 38 publications

Localization and differential expression of arginase II in the kidney of male and female mice

Localization and differential expression of arginase II in the kidney of male and female mice

Sex-differential expression of ornithine aminotransferase in the mouse kidney

Sex Hormones and Macronutrient Metabolism

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