Influence of Dietary Copper on Serum Growth-Related Hormone Levels and Growth Performance of Weanling Pigs

Wang, Jianguo; Zhu, Xiaoyan; Guo, Yazhou; Wang, Zhe; Zhao, Bin; Yin, Yunhou; Liu, Zhaoxi

doi:10.1007/s12011-015-0574-2

Cited by 21 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been believed that the growthpromoting effects of copper are related to the GH axis and might be generated by the stimulation of GH secretions [27]. Our study observed that serum GH increased in the HC group, similar to the results of a previous study which showed that high copper levels (100 to 300 mg•kg − 1 ) can increase serum GH concentrations in weaning pigs [28]. However, high levels of copper did not affect the serum IGF-1 in our study, unlike Wang's previous study, which found that dietary copper (250 mg•kg − 1 , CuSO 4 ) increased serum IGF-1 concentrations on days 20 and 40 for weanling pigs, with the rise in serum IGF-1 partly due to increased feed intake [29].…”

Section: Discussionsupporting

confidence: 90%

Suhuai suckling piglet ionomic-metabolome responses to different dietary copper level in antibiotic free creep feed

Zhang

Zheng

Xue

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background: High levels of dietary copper may enhance co-selection in favor of antibiotic resistant bacteria and promote the spread of antibiotic resistance. This possibility necessitates further assessment of the safety and utility of supplementing feed with copper. Methods: Eighteen Suhuai sows at second parity were divided into 3 experimental groups in which their 180 suckling piglets had access to antibiotic free creep feed with different levels of copper supplementation: low copper diet (LC, 6 mg·kg -1 ), control diet (CON, 20 mg·kg -1 ), and high copper diet (HC, 300 mg·kg -1 ), which was offered ad libitum from day 14 until weaning at day 40. The growth performance, serum biochemical parameters, ionomic profiles (hair, serum, and feces), fecal significant metabolites of suckling piglets, and the correlations were analyzed. Results: In HC group, the average daily gain (ADG) and average daily feed intake (ADFI) increased during d 14 to 28 ( P < 0.05), but ADG was decreased with extension of feeding time (d 29 to 40) ( P < 0.01). Compared with the CON group, the tumor necrosis factor-α (TNF-α) ( P < 0.05) was decreased while total antioxidant capacity (T-AOC) ( P < 0.05) was increased in HC group. The hair Na ( P < 0.01) and K ( P < 0.01) concentrations were increased in HC group than CON group; hair Cu ( P < 0.01), fecal Cu ( P < 0.01) increased in the HC group than LC group. The hair Na and K were negatively correlated with serum TNF-α and fecal inosine ( P < 0.05), while positively correlated with serum insulin-like growth factors-1 (IGF-1) and T-AOC ( P < 0.05); the hair Cu was negatively correlated with serum malondialdehyde (MDA), total bile acid (TBA) and fecal putrescine, glucose-6-phosphate, fumaric acid ( P < 0.05); the fecal Cu was positively correlated with serum growth hormone ( P < 0.05), negatively correlated with fecal methionine, pantothenic acid, and uracil ( P < 0.05). Further metabolic pathway enrichment analysis showed that the hair Cu was negatively correlated with phenylalanine and tyrosine metabolism, and mitochondrial electron transport chain pathways; fecal Cu was negatively correlated with betaine metabolism, and pantothenate and CoA biosynthesis pathways. Conclusions: Dietary 300 mg·kg -1 copper altered the ion balance and further affected the body’s redox balance state and metabolic homeostasis, which adverse to the health of piglets; dietary 20 mg·kg -1 copper maintain ion homeostasis and suitable to meet the nutritional needs of suckling piglets than 6 mg·kg -1 dietary copper.

show abstract

Section: Discussionsupporting

confidence: 90%

Suhuai suckling piglet ionomic-metabolome responses to different dietary copper level in antibiotic free creep feed

Zhang

Zheng

Xue

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The third potential mechanism in terms of growth-promoting or feed efficiency-enhancing effect of high Cu supplementation could be exerted via a systemic route. Evidence has demonstrated that increasing Cu supplementation in weaning pigs could increase serum mitogenic activity, liver superoxide dismutase activity, and pituitary growth hormone mRNA concentrations ( Zhou et al, 1994a , 1994b ) and elevate serum growth hormone and insulin-like growth factor-1, as well as enhance ghrelin mRNA expression in fundic region of stomach, and growth hormone-releasing hormone and suppressing somatostatin mRNA expression levels in hypothalamus ( Yang et al, 2011 , 2012 ; Wang et al, 2016 ). These anabolic factors could be the strong determinants for Cu to promote growth and enhance feed efficiency in pigs, which may also explain the improved growth rate and feed efficiency in the overall nursery period in pigs fed diets containing Cu-MHAC in the current experiment.…”

Section: Discussionmentioning

confidence: 99%

Interactive effects of zinc and copper sources and phytase on growth performance, mineral digestibility, bone mineral concentrations, oxidative status, and gut morphology in nursery pigs

Ren¹,

Chen²,

Wedekind³

et al. 2020

Translational Animal Science

View full text Add to dashboard Cite

Abstract This study investigated the interactive effects of zinc (Zn) and copper (Cu) sources and phytase on growth performance, oxidative status, mineral digestibility, tissue mineral concentrations, and gut morphology in nursery pigs. A total of 288 weaning barrows [body weight (BW) = 5.71 ± 0.81 kg], blocked by initial BW, were randomly allotted to one of eight dietary treatments, with nine pens per treatment and four pigs per pen. The eight dietary treatments were arranged in 2 × 2 × 2 factorial design, with two Zn sources [2,000, 2,000, and 100 mg/kg Zn from zinc oxide (ZnO) during phase 1 (days 1–14) and phase 2 (days 15–28), and phase 3 (days 29–42), respectively; 100 mg/kg Zn from zinc methionine hydroxy analogue chelate (Zn-MHAC) from phases 1 to 3], two Cu sources [150, 80, and 80 mg/kg Cu from copper sulfate (CuSO4) or copper methionine hydroxy analogue chelate (Cu-MHAC) during phases 1–3, respectively], and two phytase inclusion levels (0 or 500 FTU/kg). Results showed that ZnO supplementation at 2,000 mg/kg Zn significantly increased average daily feed intake (ADFI; P = 0.01) and average daily gain (ADG; P = 0.03) during phase 1 compared to Zn-MHAC group; however, Zn-MHAC supplementation tended (P = 0.06) to improve gain to feed ratio (G:F) during phase 2 compared to ZnO group. There were no differences (P > 0.10) between ZnO and Zn-MHAC groups in terms of ADG, ADFI, and G:F during the entire nursery period. Compared with CuSO4, Cu-MHAC tended to increase ADG (P = 0.07) and G:F (P = 0.08) during the entire nursery period. Phytase supplementation significantly increased ADG (P < 0.01), ADFI (P < 0.01), and G:F (P < 0.01) during the entire nursery period compared with no phytase supplementation. There was a significant interaction (P < 0.01) between Zn source and phytase on standardized total tract digestibility (STTD) of phosphorus (P), whereas there was no interaction (P = 0.21) between Cu sources and phytase on STTD of P. However, there was a significant interaction between Cu sources and phytase on calcium (Ca; P = 0.02) and P (P = 0.03) concentrations in metacarpal bones and G:F in phase 2 (P = 0.09). Furthermore, pigs fed diets containing Zn-MHAC tended to have lower ileum villus width (P = 0.07), compared with those fed diets containing ZnO, and pigs fed diets containing Cu-MHAC tended to have lower plasma malondialdehyde concentration (P = 0.10) compared with those fed diets containing CuSO4. In conclusion, under the conditions of the current study, ZnO supplementation at 2,000 mg/kg Zn was only effective in the first 2 wk postweaning, whereas Zn-MHAC supplementation at 100 mg/kg Zn could achieve better feed efficiency during phase 2 compared to pharmacological levels of ZnO, therefore, leading to no difference of growth performance in the entire nursery period. Low levels of Zn-MHAC may improve phytase efficacy on degrading phytate P compared to pharmacological levels of ZnO. Cu-MHAC may be more effective to promote growth compared to CuSO4, which may be partially driven by reduced oxidative stress. Results also indicated that Cu-MHAC might exert a synergistic effect with phytase on improving feed efficiency and bone mineralization.

show abstract

“…Copper is an essential trace element in animals that is beneficial to hematopoiesis, growth, metabolism, and various important physiological activities of life [1]. For a long time, copper has been added to swine diets at high concentrations to reduce infection and promote growth, especially for weanling pigs [2,3]. However, high concentrations of copper in the diet may lead to organ damage of swine and pose a threat to the environment [4,5].…”

Section: Introductionmentioning

confidence: 99%

Different Sources of Copper Effect on Intestinal Epithelial Cell: Toxicity, Oxidative Stress, and Metabolism

Wen

Lin

et al. 2019

Metabolites

View full text Add to dashboard Cite

Copper (Cu) is widely used in the swine industry to improve the growth performance of pigs. However, high doses of copper will induce cell damage and toxicity. The aim of this study was to evaluate toxicity, bioavailability, and effects on metabolic processes of varying copper sources using porcine intestinal epithelial cells (IPEC-J2) as a model. The IPEC-J2 were treated with two doses (30 and 120 μM) of CuSO4, Cu Glycine (Cu-Gly), and Cu proteinate (Cu-Pro) for 10 h, respectively. Cell damage and cellular copper metabolism were measured by the changes in cell viability, copper uptake, oxidative stress biomarkers, and gene/protein expression levels. The results showed that cell viability and ratio of reduced and oxidized glutathione (GSH/GSSG) decreased significantly in all treatment groups; intracellular copper content increased significantly in all treatment groups; total superoxide dismutase (SOD) activity increased significantly in the 120 μM exposed groups; SOD1 protein expression levels were significantly upregulated in 30 μM Cu-Pro, 120 μM Cu-Gly, and 120 μM Cu-Pro treatment groups; intracellular reactive oxygen species (ROS) generation and malondialdehyde (MDA) content increased significantly in 30 μM treatment groups and 120 μM CuSO4 treatment group. CTR1 and ATP7A gene expression were significantly downregulated in the 120 μM exposed groups. While upregulation of ATOX1 expression was observed in the presence of 120 μM Cu-Gly and Cu-Pro. ASCT2 gene expression was significantly upregulated after 120 μM Cu-Glycine and CuSO4 exposure, and PepT1 gene expression was significantly upregulated after Cu-Pro exposure. In addition, CTR1 protein expression level decreased after 120 μM CuSO4 and Cu-Gly exposure. PepT1 protein expression level was only upregulated after 120 μM Cu-Pro exposure. These findings indicated that extra copper supplementation can induce intestinal epithelial cell injury, and different forms of copper may have differing effects on cell metabolism.

show abstract

Influence of Dietary Copper on Serum Growth-Related Hormone Levels and Growth Performance of Weanling Pigs

Cited by 21 publications

References 19 publications

Suhuai suckling piglet ionomic-metabolome responses to different dietary copper level in antibiotic free creep feed

Suhuai suckling piglet ionomic-metabolome responses to different dietary copper level in antibiotic free creep feed

Interactive effects of zinc and copper sources and phytase on growth performance, mineral digestibility, bone mineral concentrations, oxidative status, and gut morphology in nursery pigs

Different Sources of Copper Effect on Intestinal Epithelial Cell: Toxicity, Oxidative Stress, and Metabolism

Contact Info

Product

Resources

About