BackgroundHeat stress (HS) jeopardizes intestinal barrier functions and augments intestinal permeability in pigs. However, whether HS-induced maternal microbial and metabolic changes in primiparous sows during late gestation remains elusive. We present here, a study investigating the fecal microbial and metabolic responses in late gestational primiparous sows when exposed to HS.MethodsTwelve first-parity Landrace × Large White F1 sows were randomly assigned into two environmental treatments including the thermoneutral (TN) (18–22 °C; n = 6) and HS (28–32 °C; n = 6) conditions. Both treatments were applied from 85 d of gestation to farrowing. The serum and feces samples were collected on d 107 of gestation, for analyses including intestinal integrity biomarkers, high-throughput sequencing metagenomics, short-chain fatty acid (SCFA) profiles and nontargeted metabolomics.ResultsOur results show that HS group has higher serum Heat shock protein 70 (HSP70), lipopolysaccharide (LPS) and lipopolysaccharide-binding protein (LBP) levels. The gut microbial community can be altered upon HS by using β-diversity and taxon-based analysis. In particular, the relative abundance of genera and operational taxonomic units (OTUs) related to Clostridiales and Halomonas are higher in HS group, the relative abundance of genera and OTUs related to Bacteroidales and Streptococcus, however, are lower in HS group. Results of metabolic analysis reveal that HS lowers the concentrations of propionate, butyrate, total SCFA, succinate, fumarate, malate, lactate, aspartate, ethanolamine, β-alanine and niacin, whereas that of fructose and azelaic acid are higher in HS group. These metabolites mainly affect propanoate metabolism, alanine, aspartate and glutamate metabolism, phenylalanine metabolism, β-alanine metabolism, pantothenate and CoA biosynthesis, tricarboxylic acid cycle (TCA) and nicotinate and nicotinamide metabolism. Additionally, correlation analysis between significant microbes and metabolites indicated that the HS-induced microbiota shift is likely the cause of changes of intestinal metabolism.ConclusionsTaken together, we reveal characteristic structural and metabolic changes in maternal gut microbiota as a result of late gestational HS, which could potentially provide the basis for further study on offspring gut microbiota and immune programming.

Unabsorbed copper accumulates in the hindgut of pigs that consume high levels of dietary copper, which enhances the coselection of antibiotic-resistant bacteria and is considered detrimental to the environment and to porcine health. In our study, a combination of 16S rRNA pyrosequencing and nontargeted metabolomics was used to investigate the microbiome-metabolome responses to dietary copper levels in the hindgut of suckling piglets. The results showed that the dietary copper level affected the abundance of several Clostridia genera and that the relative abundance of butyrate-producing bacteria, such as Coprococcus , Roseburia , and Acidaminococcus , was reduced in the 300 mg kg −1 (high) Cu group. Metabolomic analysis revealed that dietary copper levels affected protein and carbohydrate metabolites, protein biosynthesis, the urea cycle, galactose metabolism, gluconeogenesis, and amino acid metabolism (including the metabolism of arginine, proline, β-alanine, phenylalanine, tyrosine, and methionine). Furthermore, Pearson’s correlation analysis showed that the abundance levels of Coprococcus (family Lachnospiraceae ) and operational taxonomic unit (OTU) 18 (family Ruminococcaceae ) were positively correlated with energy metabolism pathways (gluconeogenesis, glycolysis, and the pentose phosphate pathway). The abundance of Streptococcus was negatively correlated with amino acid metabolism pathways (protein biosynthesis, glycine, serine, threonine, methionine, phenylalanine, and tyrosine metabolism), and OTU583 and OTU1067 (family Rikenellaceae ) were positively correlated with amino acid metabolism pathways. These results suggest that the copper levels consumed by LC (low-copper group) versus HC (high-copper group) animals alter the composition of the gut microbiota and modulate microbial metabolic pathways, which may further affect the health of suckling piglets. Electronic supplementary material The online version of this article (10.1007/s00253-018-9533-0) contains supplementary material, which is available to authorized users.

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The present study was aimed to compare growth performance, gonadal development, carcass characteristic and meat quality among SuHuai entire, immunocastrated and surgically castrated female pigs. Eighty 12-week-old SuHuai gilts were randomly assigned to the following four treatment groups with four pigs per pen and five replicate pens per treatment: control (PBS injection), two-dose Improvac regimen (V2), three-dose Improvac regimen (V3) and surgical castration (S). All pigs had ad libitum access to standard diets and water. The performance and occurrence of oestrus were recorded in the trial. Backfat thickness and loin eye muscle area were recorded ultrasonically. The pigs were slaughtered to measure the weight and size of the uterus, and the weight and activity of the ovaries, to determine the serum concentrations of progesterone, and to evaluate the quality of carcass and meat. There were no significant differences in growth performance among the four groups, except for the V2, which had a better average daily feed intake (P < 0.05). Carcass and meat quality were comparable among the four groups; however, the intramuscular fat in control was higher than the V2 and V3 (P < 0.01). Mean ovary and uterus weights and oestrus of female pigs in V2 and V3 were significantly (P < 0.01) lower than in control, but the difference between the V2 and V3 was not significant. The concentration of progesterone in the control was significantly higher than that in the V2, V3 and S (P < 0.05). These results indicated that immunocastration successfully suppressed gonadal development and had no adverse effects on growth performance, or carcass or meat-quality traits in Chinese SuHuai female pigs.