The present study was conducted to study the impact of serotonin, tryptophan and Optifeed adding in drinking water on behavior characterize, body performance, sensory evaluation of breast and thigh meat, some biochemical parameters of blood and immune status against some diseases by ELISA of broiler . 400 unsexed chicks one day old were randomly distributed into 4 treatments and 4 replicates for each treatment, are: T0 (control standard drinking water DW), T1 (0.5 g tryptophan/1 L DW, T2 (2 mg serotonin/1 L DW, T3 (0.5 g Optifeed®/1 L DW). The birds were reared for 42 days and behavior traits of birds controlled by erected cameras upon all replicates. The analyzed results were significantly improved behavioral characteristics, body performance (body weight and gain, feed intake, FCR, water consumption, uniformity, liver, and gizzard%), feasibility (economic profit), sensory evaluation (aroma, taste, tenderness, juiciness, and acceptance) of cocked breast and thigh meat, blood serum concentrations (total protein, globulin, highdensity lipoprotein (HDL), serotonin, thyroxin (T4), tryptophan), and immunological ELISA titers against Newcastle, Gumboro, and Infectious bronchitis viral diseases. Otherwise, significantly decreased mortality, and blood serum concentrations, (triglyceride, low-density lipoprotein (LDL), and corticosterone) in all treatments of water additives compared with the control. The addition of Optifeed® and serotonin positively affects in all experimental characteristics than others especially improved behavioral characterize of broiler during the rearing periods.