Influence of Dietary Raw Green Gram <i>(Phaseolus aureus Roxb) </i>and Green Gram Trypsin Inhibitors on the Activity of Certain Protein Metabolism Enzymes in Rats

Abstract

The influence of a raw green gram (RGG) diet, an autoclaved green gram (AGG) diet and green gram trypsin inhibitors (GGTI) incorporated in AGG diet on urinary and blood urea and creatinine levels in rats was studied. The activities of certain liver enzymes of pathways associated with protein or amino acid metabolism were also studied. The levels of urea and creatinine in urine and blood were found to be significantly increased in rats fed the RGG and GGTI-incorporated AGG diets when compared to the animals fed with the AGG diet. The levels of enzyme activities of arginase, ornithine transcarbamoylase, aspartate amino-transferase and alanine aminotransferase were also found to be significantly increased along with that of urea and creatinine. The possible role of GGTI on the altered levels of the above-mentioned parameters is discussed.

Assessment of serum biochemistry in West African Dwarf (WAD) does administered varying levels of medroxyl–progesterone acetate (MPA), an estrus synchronizing drug

“…The increase or decrease in the urea and creatinine values during drug administration and drug withdrawal agrees with previous findings of Sathyamorthy et al (1981). They reported increase in serum urea and creatinine in rat.…”

Influence of black gram(Vigna mungo) trypsin inhibitory fraction on the hepatic protein catabolism in male albino mice

“…This indicates the enhanced activity of the enzymes of the urea cycle and hence the increased amino acid catabolism. Our earlier studies [5] showed similar effects when rats were fed raw green gram and green gram trypsin inhibitor. The amino acids, aspartate and glutamate, are used for urea biosynthesis.…”

“…The type of changes in protein catabolism in liver due to pancreatic hypersecretion is not known. The influence of raw legumes and trypsin inhibitors on the activity of some protein catabolism enzymes in rats [4,5] and chicks [4] has been reported. Increased levels of urea cycle enzymes, blood urea, creatinine as well as an elevated excretion of urinary urea and creatinine have been reported in animals fed raw legumes [4,5].…”

Effect of dietary green gram trypsin inhibitor and raw green gram meal (Phaseolus aureus roxb) on the pancreatic activity in rats

“…The trypsin inhibitory fraction of raw green gram was isolated by the method of Kakade et al [11] with slight modification, as illustrated in our previous publication [27].…”