Influence of different elicitors on BIA production in Macleaya cordata

Abstract

Sanguinarine (SAN) and chelerythrine (CHE) have been widely used as substitutes for antibiotics for decades. For a long time, SAN and CHE have been extracted from mainly Macleaya cordata, a plant species that is a traditional herb in China and belongs to the Papaveraceae family. However, with the sharp increase in demand for SAN and CHE, it is necessary to develop a new method to enhance the supply of raw materials. Here, we used methyl jasmonate (MJ), salicylic acid (SA) and wounding alone and in combination to stimulate aseptic seedlings of M. cordata at 0 h, 24 h, 72 h and 120 h and then compared the differences in metabolic profiles and gene expression. Ultimately, we found that the effect of using MJ alone was the best treatment, with the contents of SAN and CHE increasing by 10- and 14-fold, respectively. However, the increased SAN and CHE contents in response to combined wounding and MJ were less than those for induced by the treatment with MJ alone. Additionally, after MJ treatment, SAN and CHE biosynthetic pathway genes, such as those encoding the protopine 6-hydroxylase and dihydrobenzophenanthridine oxidase enzymes, were highly expressed, which is consistent with the accumulation of SAN and CHE. At the same time, we have also studied the changes in the content of synthetic intermediates of SAN and CHE after elicitor induction. This study is the first systematic research report about using elicitors to increase the SAN and CHE in Macleaya cordata.

Sanguinarine Exhibits Antiviral Activity against Porcine Reproductive and Respiratory Syndrome Virus via Multisite Inhibition Mechanisms

“…HM853673) isolated in 2006 by our laboratory [ 16 ] was amplified and titered in MARC-145 cells. Chelerythrine, sanguinarine, and BE60 (the drug with chelerythrine and sanguinarine as the primary active component) were gifted by Dr. Jianguo Zeng at Hunan Agricultural University, China [ 17 ].…”

Increasing Amounts of Secondary Metabolites and Medicinal Compounds in Callus Culture of Moringa Oleifera (Lam.) Using Abiotic Elicitors

“…Elicitation is among plants' most effective strategies for increasing secondary metabolite biosynthesis [32]. But most biotic or abiotic stresses often have a detrimental effect on biomass growth in most agricultural systems.…”

Eco-friendly approaches to phytochemical production: elicitation and beyond

“…There are numerous benefits of using plant tissue culture such as it is independent of the environmental or geographic conditions, production and the quality can be regulated, life cycle of plant gets shortened in comparison to the field growing plant and escapes the use of land resources. In fact, from over a decade, some plant bio actives like shikonin, ginseng saponins and paclitaxel have been synthesized using this methodology [ 6 ]. Besides this, many studies have been targeted on strategies that enhances plant in-vitro cultures productiveness, which includes optimization of media, cell line selection, cell immobilization, precursor feeding and elicitation.…”