Influence of Different pH Conditions and Phosphate Sources on Phosphate Solubilization by Pantoea agglomerans DSM3493

Walpola, Buddhi Charana; Keum, Mi-Jung; Yoon, Min-Ho

doi:10.7745/kjssf.2012.45.6.998

Cited by 3 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, in vitro solubilizing potential assessments were susceptible to the influence of many factors, as they significantly modify the living conditions of microorganisms. The growth temperature, pH, and nutritional composition of the culture medium could affect growth and metabolism (e.g., Gibson and Mitchell, 2004;Walpola et al, 2012;Tan and Ramamurthi, 2014;Yang et al, 2018), including the ability of isolates to produce organic acids or phosphatase enzymes directly associated with the potential of the microorganism to release P from forms where it is immobilized as CaHPO 4 , Ca 3 (PO 4 ) 2 , AlPO 4 , and FePO 4 .…”

Section: Solubilization Results Obtained Using In Vitro Methodologies Are Not Consistent With the Hydroponic Systemmentioning

confidence: 99%

Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO4 by Microbial Strains Using Glycine max as a Model

et al. 2021

View full text Add to dashboard Cite

The sustainable development of agriculture can be stimulated by the great market availability of bio-inputs, including phosphate-solubilizing microbial strains. However, these strains are currently selected using imprecise and questionable solubilization methodologies in solid or liquid media. We hypothesized that the hydroponic system could be a more efficient methodology for selecting phosphate-solubilizing strains as plant growth promoters. This methodology was tested using the plant Glycine max as a model. The growth-promoting potential of the strains was compared with that of the Biomaphos® commercial microbial mixture. The obtained calcium phosphate (CaHPO4) solubilization results using the hydroponic system were inconsistent with those observed in solid and liquid media. However, the tests in liquid medium demonstrated poor performances of Codinaeopsis sp. (328EF) and Hamigera insecticola (33EF) in reducing pH and solubilizing CaHPO4, which corroborates with the effects of biotic stress observed in G. max plants inoculated with these strains. Nevertheless, the hydroponic system allowed the characterization of Paenibacillus alvei (PA12), which is also efficient in solubilization in a liquid medium. The bacterium Lysinibacillus fusiformis (PA26) was the most effective in CaHPO4 solubilization owing to the higher phosphorus (P) absorption, growth promotion, and physiological performance observed in plants inoculated with this bacterium. The hydroponic method proved to be superior in selecting solubilizing strains, allowing the assessment of multiple patterns, such as nutritional level, growth, photosynthetic performance, and anatomical variation in plants, and even the detection of biotic stress responses to inoculation, obtaining strains with higher growth promotion potential than Biomaphos®. This study proposed a new approach to confirm the solubilizing activity of microorganisms previously selected in vitro and potentially intended for the bio-input market that are useful in P availability for important crops, such as soybeans.

show abstract

Section: Solubilization Results Obtained Using In Vitro Methodologies Are Not Consistent With the Hydroponic Systemmentioning

confidence: 99%

Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO4 by Microbial Strains Using Glycine max as a Model

et al. 2021

View full text Add to dashboard Cite

show abstract

“…), SAF11 (Brevibacillus sp. ), and SAC36 (Bacillus velezensis), isolated from the production environment with a 30year history of cultivation had solubilization values (WALPOLA et al, 2012;YANG et al, 2018;REIS et al, 2021).…”

Section: Rhizobacteria Isolated and Selected From Glycine Max Bait Pl...mentioning

confidence: 99%

Plant growth-promoting rhizobacteria isolated from cultivated soils using Glycine max L. plants as bait

Fiuza,

Vitorino,

Silva

et al. 2024

Cienc. Rural

View full text Add to dashboard Cite

The current production of major crops, such as Glycine max L., has become increasingly adept on the use of bio-inputs, such as application of plant growth-promoting inocula. However, the evaluation of the potential of bacterial isolates from soils with different time histories of agricultural use are still scarce, and methods for isolation and testing of multifunctional microorganisms need to be continuously innovated and improved. Thus, we used G. max bait plants to attract rhizobacteria from soils obtained from three areas with different time histories of soybean cultivation. We evaluated the influence of the management and characteristics of soils on the phosphate-solubilizing bacterial population. We then tested the multifunctional potential of the isolated rhizobacteria for calcium phosphate (CaHPO4) and iron phosphate (FePO4) solubilization, considering their potential as microbial inoculants in the future. The use of bait plants enabled the isolation of 139 phosphate solubilizing rhizobacteria, including four strains with multifunctional potential. The largest number of solubilizing bacteria was obtained from the interaction of bait plants with soil from an area with a history of soybean cultivation for thirty consecutive years. The high concentration of P, Fe, and K found in the soil were associated with this occurrence. In the in vitro tests, the pH values of the culture media had a moderate negative relationship with the amounts of P made available by the isolates, indicating that other processes besides the availability of organic acids, may underlie the solubilizing action of the isolates. The rhizobacteria SAF9 (Brevibacillus sp.), SAF11 (Brevibacillus sp.), BRC11 (Pseudomonas fluorescens), and SAC36 (Bacillus velezensis) stood out as multifunctional and are indicated within a perspective of obtaining bioinoculants to promote plant growth directly, indirectly, or synergistically, contributing to increase the range of bio-inputs for soybean cultivation and more sustainable agricultural practices.

show abstract

Contribution of native phosphorous-solubilizing bacteria of acid soils on phosphorous acquisition in peanut (Arachis hypogaea L.)

et al. 2017

View full text Add to dashboard Cite

The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through biofertilization in peanut (Arachis hypogaea L.) growth and P acquisition. One hundred six numbers of soil samples with pH ≤ 5.50 were collected from five districts of Odisha viz., Balasore, Cuttack, Khordha, Keonjhar, and Mayurbhanj. One bacterial isolate from each district were selected and analyzed for their P solubilization efficiency in National Botanical Research Institute Phosphate broths with Ca, Al, and Fe-complexed phosphates. CTC12 and KHD08 transformed more amount of soluble P from Ca-P (CTC12 393.30 mg/L; KHD08 465.25 mg/L), Al-P (CTC12 40.00 mg/L; KHD08 34.50 mg/L), Fe(III)-P (CTC12 175.50 mg/L; KHD08 168.75 mg/L), and Fe(II)-P (CTC12 47.40 mg/L; KHD08 42.00 mg/L) after 8 days of incubation. The bioconversion of P by all the five strains in the broth medium followed the order Ca-P > Fe(III)-P > Fe(II)-P > Al-P. The identified five strains were Bacillus cereus BLS18 (KT582541), Bacillus amyloliquefaciens CTC12 (KT633845), Burkholderia cepacia KHD08 (KT717633), B. cepacia KJR03 (KT717634), and B. cepacia K1 (KM030037) and further studied for biofertilization effects on peanut. CTC12 and KHD08 enhanced the soil available P around 65 and 58% and reduced the amount of each Al about 79 and 81%, respectively, over the uninoculated control pots in the peanut rhizosphere. Moreover, all tested PSB strains could be able to successfully mobilize P from inorganic P fractions (non-occluded Al-P and Fe-P). The strains CTC12 and KHD08 increased the pod yield (114 and 113%), shoot P (92 and 94%), and kernel P (100 and 101%), respectively, over the control. However, B. amyloliquefaciens CTC12 and B. cepacia KHD08 proved to be the potent P solubilizers in promoting peanut growth and yield.

show abstract

Influence of Different pH Conditions and Phosphate Sources on Phosphate Solubilization by Pantoea agglomerans DSM3493

Cited by 3 publications

References 18 publications

Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO4 by Microbial Strains Using Glycine max as a Model

Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO4 by Microbial Strains Using Glycine max as a Model

Plant growth-promoting rhizobacteria isolated from cultivated soils using Glycine max L. plants as bait

Contribution of native phosphorous-solubilizing bacteria of acid soils on phosphorous acquisition in peanut (Arachis hypogaea L.)

Contact Info

Product

Resources

About