Influence of diffuse reflectance measurement accuracy on the scattering coefficient in determination of optical properties with integrating sphere optics (a secondary publication)

Abstract

An estimation error of the scattering coefficient of hemoglobin in the high absorption wavelength range has been observed in optical property calculations of blood-rich tissues. In this study, the relationship between the accuracy of diffuse reflectance measurement in the integrating sphere and calculated scattering coefficient was evaluated with a system to calculate optical properties combined with an integrating sphere setup and the inverse Monte Carlo simulation. Diffuse reflectance was measured with the integrating sphere using a small incident port diameter and optical properties were calculated. As a result, the estimation error of the scattering coefficient was improved by accurate measurement of diffuse reflectance. In the high absorption wavelength range, the accuracy of diffuse reflectance measurement has an effect on the calculated scattering coefficient.

SERS-based immunoassay on a plasmonic syringe filter for improved sampling and labeling efficiency of biomarkers

“…, broad band). Although the absorption measurement on a 2-D substrate is challenging due to the measurement conditions involving a strong absorber paper material, 51,52 the absorption pattern of the plasmonic paper clearly indicated the presence of a certain degree of locally aggregated AuNPs to induce plasmonic coupling. 53–55…”

“…As randomly aggregated AuNPs are abundantly distributed across filter paper, numerous spacings/gaps between AuNPs readily govern the overall wavelength shift and overlap (i.e., broad band). Although the absorption measurement on a 2-D substrate is challenging due to the measurement conditions involving a strong absorber paper material, 51,52 the absorption pattern of the plasmonic paper clearly indicated the presence of a certain degree of locally aggregated AuNPs to induce plasmonic coupling. [53][54][55] Dynamic light scattering (DLS) and UV-vis extinction spectrophotometry were used to characterize the synthesized ERLs.…”

Sandwiching analytes with structurally diverse plasmonic nanoparticles on paper substrates for surface enhanced Raman spectroscopy

“…Although obtaining strong absorption patterns from 2-dimensional substrates is difficult due to the nature of reectance measurement conditions particularly with a strong absorber paper surface, 40,41 the appearance of detectable additional peaks at longer wavelengths with respect to the NPs in solution clearly indicates the presence of local aggregates of plasmonic NPs on the lter paper (Fig. S2 †).…”