Influence of eCG and breed on the number of oocytes collected and the production of in vitro embryos of young goats during the reproductive season

Montes-Quiroz, Gabriela Lisset; Sánchez-Dávila, Fernando; Domínguez-Díaz, David; Vázquez-Armijo, José Fernando; Grizelj, Juraj; Ledezma-Torres, Rogelio Alejandro; Cervantes-Vega, Rubén; Arce-Vázquez, Nestor; Brenner, Estela Garza; Barragán, Hugo Bernal

doi:10.1007/s11250-019-01972-5

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…Follicles number and their types vary between mammal species, and within the same species (Batista et al, 2014). Beside these, follicle size is also important because it interferes with the efficiency of oocyte collection (Montes-Quiroz et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Cattle Ovaries and Follicles by Histological Analysis for Potential in Vitro Production of Embryos in Tropical Conditions

Mahzabin¹,

Khandoker²,

Husain³

et al. 2020

Trop Subtrop Agroecosyst

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Background. Reproduction is important in animal production, and follicle evaluation is a remarkable reproductive aspect by which ovaries can be accurately evaluated since ovarian follicles are the organ's functional units and each contain an oocyte and related somatic cells. Objective. The objective of this study was to evaluate the abattoir cattle ovaries and follicles for potential in vitro production of embryos. Methodology. Ovaries were collected and categorized based on the presence of corpus luteum (CL; with or without). Thereafter, ovaries were processed and follicles were counted through naked eye estimation as well histologically. Data were analyzed using the GLM procedure in SAS. Also, relationships between follicles in both types of ovaries were calculated using Pearson correlations. Results. From a total of sixteen cattle ovaries, 62.5% had absence of CL whereas 37.5% ovaries had CL. Regarding morphometric measurements, ovaries with CL were 48% higher (P < 0.05) in weight than those ovaries without CL. Correlations between length and weight in ovaries without CL were high (r= 0.87) and, diameter and weight in ovaries with CL were low (r= 0.41). Results exposed that the amount of follicles with 2-6 mm diameter were significantly (P < 0.05) higher (61% more) in ovaries without CL compared to those with CL. Primary and secondary follicles presence were higher (P < 0.05) in ovaries without CL (120% more) than in those with CL (89%). Greater (P < 0.05) numbers of normal and lower (P < 0.05) amount of degenerated follicles were found in ovaries without CL. Implications. Overall, our data suggest that ovaries without CL are a potential source for quality follicles and this study provides new insights that may serve as the baseline information to produce high-quality oocyte with potential use for in vitro production of tropical cattle. Conclusions. This study corroborated that CL influences growth and development of the follicles and cell degeneration. As a result, the developmental competence of oocytes and embryo production will be negatively affected by the ovaries with CL. Ovaries without CL will provide quality follicles and cumulus-oocyte complexes.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Cattle Ovaries and Follicles by Histological Analysis for Potential in Vitro Production of Embryos in Tropical Conditions

Mahzabin¹,

Khandoker²,

Husain³

et al. 2020

Trop Subtrop Agroecosyst

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“…In sheep, a similar result has been reported by Gibson et al (2004) where eCG induced an earlier surge of the pre‐ovulatory LH. It has been reported that ovarian stimulation can be induced either using FSH or eCG in several species including the cow (Small et al, 2009), sheep (Cognie, 1999), pigs (Manjarin et al, 2009), goats (Montes‐Quiroz et al, 2019), mouse (Crispo et al, 2021) and rats (Popova et al, 2002), and it not only increased the number of follicles but also increased the transition of small to medium follicles (Sarwar et al, 2020). Thus, it is plausible to speculate that the FSH‐like activity of eCG might have influenced the stimulation of follicular growth and increased the secretion of E 2 by the pre‐ovulatory follicles as observed in the present experiment.…”

Section: Discussionmentioning

confidence: 99%

Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when oestrus was induced during low‐breeding season in Beetal goats

Sharif

Hassan

Arshad

et al. 2022

Reprod Domestic Animals

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The objectives of the experiment were to determine the effect of two doses of equine chorionic gonadotropin (eCG) in a standard synchronization protocol based on a short‐term progesterone (P4) priming on ovarian structures and haemodynamics, concentrations of steroid hormones and prolificacy rate when oestrus was induced during low‐breeding season (LBS) in Beetal dairy goats. We hypothesized that inclusion of eCG in a short‐term P4 priming‐based synchronization protocol would increase the blood perfusion to ovarian structures leading to enhance oestrous and ovulatory responses and prolificacy rate in goats. Forty‐two multiparous acyclic goats were blocked by body condition and, within block, assigned randomly to receive saline as control (CON), low eCG (L‐eCG; 300 IU) or high eCG (H‐eCG; 600 IU) dose. Initially, a controlled internal drug release (CIDR) device was placed in the anterior vagina on d −8, followed by removal of CIDR on d −3, concurrent with the administration of PGF2α and eCG according to their respective treatments. Goats were monitored for oestrous response. B‐mode and Doppler ultrasonography was performed with 12‐h interval, starting from day −3 until natural breeding (day 0), and then on days 5, 10, 15 and 20 post‐breeding to monitor follicular and luteal dynamics and blood flow, respectively. Blood was sampled at 0, 12, 24, 36 and 60 h after CIDR removal to quantify plasma concentrations of estradiol‐17β (E2), whereas plasma concentrations of P4 were assayed at days 5, 10, 15 and 20 after breeding. Pregnancy and prolificacy rates were determined at day 30 and 150 after breeding, respectively. Data were analysed with mixed‐effects models, and orthogonal contrasts were used to evaluate the effect of treatment [Con vs. (½ L‐eCG + ½ H‐eCG)] and dose of eCG (L‐eCG vs. H‐eCG). Data are presented in sequence as CON, L‐eCG, H‐eCG (LSM ± SEM). The oestrous intensity score (152.9 vs. 182.7 vs. 186.5 ± 15.1; p = .02) was greater in eCG‐treated goats as compared to CON. Administration of eCG reduced the intervals to standing oestrus (66.2 vs. 41.8 vs. 48.9 h ± 5.5; p = .05), breeding (70.2 vs. 44.4 vs. 45.4 h ± 4.5; p = .03) and ovulation (84.5 vs. 61.2 vs. 63.4 h ± 6.2; p = .05) compared with CON goats. The mean growth rate of pre‐ovulatory follicle was greater (1.11 vs. 1.49 vs. 1.45 mm ± 0.08; p = .01) in eCG‐treated goats resulting in an increased diameter of pre‐ovulatory follicle (6.27 vs. 7.20 vs. 7.31 mm ± 0.07; p < .01) and corpora lutea (6.75 vs. 8.26 vs. 8.07 mm ± 0.42; p = .04) than CON. The mean follicular blood flow did not differ among treatments; however, the mean luteal blood flow was greater in L‐eCG‐treated goats (0.81 vs. 1.61 vs. 1.07 cm2 ± 0.12; p = .001). The mean concentrations of E2 (4.03 vs. 5.21 vs. 4.78 pg/ml ± 0.42; p = .04) and P4 (4.85 vs. 6.39 vs. 6.22 ng/ml ± 0.34; p = .04) were greater in eCG‐treated goats. The twinning rate did not differ between treatments; nevertheless, prolificacy rate was greater (p = .04) in L‐eCG‐treated goats. Collectively, our data sugges...

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“…Estrous induction began to develop in goats and sheep for more than 50 years, where injected progesterone began to be used daily, until today with the use of two types of vaginal devices: vaginal sponge and delivery device. Controlled (CIDR), each having its advantages and disadvantages [10,11]. The response in each of the devices has been accompanied by secondary hormones of intramuscular application that favor the development of the follicles, the synchronization of them for their ovulation and that these become corpus luteum with adequate size and with a sustainable production of progesterone.…”

Section: Perspectives and Advances In The Study Of The Estrous Cycle mentioning

confidence: 99%

Reproduction in Small Ruminants (Goats)

Sánchez-Dávila¹,

Muñoz²

2021

Animal Reproduction in Veterinary Medicine

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The exploitation of small ruminants (goat and sheep) has always been linked to the development of human civilizations, where they have mainly fed on their derived products such as milk and meat. Currently, the sheep population is around 1 billion head concentrated above 50% in three countries, China, Australia, and New Zealand, contrary to goats with around 720 million heads, distributed mainly in Asia, Africa, and South America. Both species have similar characteristics in some anatomical aspects (a pair of nipples), gestation period (150 days), and presence of seasonal anestrus, differing in terms of magnitude and depth and presence of the male effect. However, they are completely different in feeding habits, nutrient needs, and grazing systems, with differences in terms of the female’s reproductive tract, among other characteristics. Currently, the study of reproduction has intensified over the years in the goats and its counterpart that is the buck. Therefore, in the following topics, the importance of global reproduction of the goat will be discussed, considering that progress has been made today in the application of third generation reproductive techniques and that today they are already consolidated and developed in the bovine species.

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Influence of eCG and breed on the number of oocytes collected and the production of in vitro embryos of young goats during the reproductive season

Cited by 3 publications

References 33 publications

Evaluation of Cattle Ovaries and Follicles by Histological Analysis for Potential in Vitro Production of Embryos in Tropical Conditions

Evaluation of Cattle Ovaries and Follicles by Histological Analysis for Potential in Vitro Production of Embryos in Tropical Conditions

Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when oestrus was induced during low‐breeding season in Beetal goats

Reproduction in Small Ruminants (Goats)

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