Influence of entomopathogenic nematodes, symbiotic bacteria and ascarosides on the dispersal behaviour of Meloidogyne incognita

Dai, Kang; Cao, Li; Huang, Zhihua; Gu, Xinghui; Cui, Yonghe; Li, Jiangzhou; Li, Yuling; Xu, Chengti; Richou, Han

doi:10.1163/15685411-bja10184

Cited by 2 publications

(1 citation statement)

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“…So, novel methods for the control of harmful plant nematodes are constantly explored. Based on its environmental friendship, biological control method receives increasing attention as the most potent plant nematode control measures for maintaining the sustainable development of green agriculture ( Gunasinghe et al, 2015 ; Kwak et al, 2018 ; Sun et al, 2021 ; Dai et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Microbial landscapes of the rhizosphere soils and roots of Luffa cylindrica plant associated with Meloidogyne incognita

et al. 2023

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IntroductionThe root-knot nematodes (RKN), especially Meloidogyne spp., are globally emerging harmful animals for many agricultural crops.MethodsTo explore microbial agents for biological control of these nematodes, the microbial communities of the rhizosphere soils and roots of sponge gourd (Luffa cylindrica) infected and non-infected by M. incognita nematodes, were investigated using culture-dependent and -independent methods.ResultsThirty-two culturable bacterial and eight fungal species, along with 10,561 bacterial and 2,427 fungal operational taxonomic units (OTUs), were identified. Nine culturable bacterial species, 955 bacterial and 701 fungal OTUs were shared in both four groups. More culturable bacterial and fungal isolates were detected from the uninfected soils and roots than from the infected soils and roots (except no fungi detected from the uninfected roots), and among all samples, nine bacterial species (Arthrobacter sp., Bacillus sp., Burkholderia ambifaria, Enterobacteriaceae sp., Fictibacillus barbaricus, Microbacterium sp., Micrococcaceae sp., Rhizobiaceae sp., and Serratia sp.) were shared, with Arthrobacter sp. and Bacillus sp. being dominant. Pseudomonas nitroreducens was exclusively present in the infested soils, while Mammaliicoccus sciuri, Microbacterium azadirachtae, and Priestia sp., together with Mucor irregularis, Penicillium sp., P. commune, and Sordariomycetes sp. were found only in the uninfected soils. Cupriavidus metallidurans, Gordonia sp., Streptomyces viridobrunneus, and Terribacillus sp. were only in the uninfected roots while Aspergillus sp. only in infected roots. After M. incognita infestation, 319 bacterial OTUs (such as Chryseobacterium) and 171 fungal OTUs (such as Spizellomyces) were increased in rhizosphere soils, while 181 bacterial OTUs (such as Pasteuria) and 166 fungal OTUs (such as Exophiala) rose their abundance in plant roots. Meanwhile, much more decreased bacterial or fungal OTUs were identified from rhizosphere soils rather than from plant roots, exhibiting the protective effects of host plant on endophytes. Among the detected bacterial isolates, Streptomyces sp. TR27 was discovered to exhibit nematocidal activity, and B. amyloliquefaciens, Bacillus sp. P35, and M. azadirachtae to show repellent potentials for the second stage M. incognita juveniles, which can be used to develop RKN bio-control agents.DiscussionThese findings provided insights into the interactions among root-knot nematodes, host plants, and microorganisms, which will inspire explorations of novel nematicides.

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Section: Introductionmentioning

confidence: 99%

Microbial landscapes of the rhizosphere soils and roots of Luffa cylindrica plant associated with Meloidogyne incognita

et al. 2023

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Meloidogyne incognita genes involved in the repellent behavior in response to ascr#9

Rao,

Dai,

Han

et al. 2024

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Meloidogyne incognita is one of the globally serious plant parasitic nematodes. New control measure is urgently needed to replace the common chemical control method. Ascarosides are pheromones regulating the nematodes’ aggregation, avoidance, mating, dispersal and dauer recovery and formation. Ascr#9, one of the ascarosides, exhibits the potential to repel M. incognita . However, the nematode genes involved in the perception of ascr# 9 signal are totally unknown. In this study, the transcriptome of ascr#9-treated second stage M. incognita juveniles (J2s) was analyzed, 44 pathways were significantly affected, multiple ligand-receptor and mucin type O-glycan were induced, and olfactory transduction was disturbed. A total of 11 highly differentially expressed genes involved in neuroactive ligand-receptor interaction and FMRFamide-like peptide related process were identified and knocked down by RNAi. The dispersal rates of M. incognita with three knocked-down genes ( flp-14 , mgl-1 and ADOR-1 ) significantly decreased, respectively, when ascr#9 was present. The results demonstrate that flp-14 , mgl-1 , and ADOR-1 are involved in the dispersal behavior of M. incognita nematodes responding to ascr#9, which promotes the interaction study between ascarosides and M. incognita , and provides new ideas for the prevention and control of M. incognita by using pheromone ascarosides. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-76370-5.

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Influence of entomopathogenic nematodes, symbiotic bacteria and ascarosides on the dispersal behaviour of Meloidogyne incognita

Cited by 2 publications

References 24 publications

Microbial landscapes of the rhizosphere soils and roots of Luffa cylindrica plant associated with Meloidogyne incognita

Microbial landscapes of the rhizosphere soils and roots of Luffa cylindrica plant associated with Meloidogyne incognita

Meloidogyne incognita genes involved in the repellent behavior in response to ascr#9

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