Influence of epidermal growth factor and transforming growth factor beta-1 on patterns of fetal mouse lung branching morphogenesis in organ culture

Chinoy, Mala R.; Zgleszewski, Steven E.; Cilley, Robert E.; Blewett, Christopher; Krummel, Thomas M.; Reisher, Samuel R.; Feinstein, Sheldon I.

doi:10.1002/(sici)1099-0496(199804)25:4<244::aid-ppul5>3.0.co;2-k

Cited by 35 publications

(18 citation statements)

References 22 publications

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“…These clinical observations are supported by in vitro studies. Addition of exogenous TGF-h1 to mouse fetal lung explants resulted in elongation of airways and reduced branching (Chinoy et al, 1998). Arrested lung morphogenesis result in transgenic mice bearing an SP-C-TGF-b 1 chimeric gene (Zhou et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Expression of transforming growth factor beta (TGF-β1) in human epithelial alveolar cells: a pro-inflammatory mediator independent pathway

et al. 2004

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Section: Introductionmentioning

confidence: 99%

Expression of transforming growth factor beta (TGF-β1) in human epithelial alveolar cells: a pro-inflammatory mediator independent pathway

et al. 2004

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“…Some of the normal and hypoplastic lung allografts were processed for light microscopic (histology) and electron microscopic (ultrastructure) evaluation as described earlier [11].…”

Section: Histology and Ultrastructurementioning

confidence: 99%

“…At different postimplantation time-points the lungs were also processed for cryomicrotomy and Immunohistochemistry [11,3].…”

Section: Immunohistochemistrymentioning

confidence: 99%

Persistent vascular defects in lung allografts attributed to defective endogenous endothelial progenitors

Chinoy

Miller

Myers

et al. 2005

Journal of Surgical Research

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“…In vitro lung organ culture has been extensively used in studying branching morphogenesis (Chinoy et al 1998;Litingtung et al 1998;Malpel et al 2000;Mahlapuu et al 2001). However, in vitro tracheal cartilage culture has not been described to date.…”

mentioning

confidence: 99%

A simple in vitro culture system for tracheal cartilage development

Park

Zhang

Choi

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

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Semi-circular tracheal cartilage is a critical determinant of maintaining architectural integrity of the respiratory airway. The current effort to understand the morphogenesis of tracheal cartilage is challenged by the lack of appropriate model systems. Here we report an in vitro tracheal cartilage system using embryonic tracheal–lung explants to recapitulate in vivo tracheal cartilage developmental processes. With modifications of a current lung culture protocol, we report a consistent in vitro technique of culturing tracheal cartilage from primitive mouse embryonic foregut for the first time. This tracheal culture system not only induces the formation of tracheal cartilage from the mouse embryonic foregut but also allows for the proper patterning of the developed tracheal cartilage. Furthermore, we show that this culture technique can be applied to culturing other types of cartilage in vertebrae, limbs, and ribs. We believe that this novel application of our in vitro culture system will facilitate the manipulation of cartilage development under various conditions and thus enabling us to advance our current limited knowledge on cartilage biology and development.

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Influence of epidermal growth factor and transforming growth factor beta-1 on patterns of fetal mouse lung branching morphogenesis in organ culture

Cited by 35 publications

References 22 publications

Expression of transforming growth factor beta (TGF-β1) in human epithelial alveolar cells: a pro-inflammatory mediator independent pathway

Expression of transforming growth factor beta (TGF-β1) in human epithelial alveolar cells: a pro-inflammatory mediator independent pathway

Persistent vascular defects in lung allografts attributed to defective endogenous endothelial progenitors

A simple in vitro culture system for tracheal cartilage development

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