Influence of Feeding Cottonseed Oil to Laying Hens on the Low Density Lipoproteins of their Eggs

Evans, Robert J.; Bauer, Dierk; Vaghefi, Simin B.; Flegal, Cal J.

doi:10.1093/jn/99.4.485

Cited by 12 publications

(3 citation statements)

References 8 publications

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“…1). With most very low density lipoproteins isolated from eggs produced by hens fed crude cottonseed oil, the first peak eluted consisted of large sized molecules and was a very big one (Evans et al, 1969). It was followed by a smaller peak of smaller sized molecules.…”

Section: Resultsmentioning

confidence: 99%

“…Evans et al (1959) observed that during paper electrophoresis the very low density lipoproteins in eggs produced by hens fed crude cottonseed oil and stored for 6 months moved almost three times as far as those in the fresh eggs. Very low density lipoproteins isolated from eggs produced by hens fed crude cottonseed oil contained more saturated fatty acids and less monenoic fatty acids and the lipoprotein molecules were larger and heavier than those isolated from normal eggs (Evans et al, 1969).…”

Section: Introductionmentioning

confidence: 84%

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The Influence of Storage of Shell Eggs Produced by Hens Fed Crude Cottonseed Oil on the Very Low Density Lipoproteins Isolated From These Eggs

1975

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Very low density lipoproteins were isolated from fresh and from 6-month old eggs laid by hens fed a diet containing crude cottonseed oil. There were no differences between the very low density lipoproteins isolated from the fresh and those from the stored eggs in the percentage of lipid extracted by ether after the action of unfolding agents, detergents, reducing agents, trypsin, Pronase, or phospholipase D. Phospholipase C released less lipid from the very low density lipoproteins of the stored eggs than from those of the fresh unless they were extracted with ether before digestion, when equal amounts were extracted. Very low density lipoproteins isolated from fresh eggs and those isolated from stored eggs contained similar percentages of lipid. The very low density lipoproteins from the stored eggs had lower isoelectric points than those from fresh eggs, but there were no differences in average size when determined by electron microscopy. Egg yolk very low density lipoproteins are normally quite stable in water solution, but those from the stored eggs showed evidence of some decomposition during prolonged ultracentrifugation in water solution in contrast to those from fresh eggs.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 84%

The Influence of Storage of Shell Eggs Produced by Hens Fed Crude Cottonseed Oil on the Very Low Density Lipoproteins Isolated From These Eggs

1975

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“…Ambient temperature can alter the degree of saturation of a solution. Variations could also result from reagent source and inherent differences in egg yolk composition; the latter varies with the interval from time of laying and use, environmental conditions, diet, genetic line, and bird species (Evans et al, 1969).…”

Section: Discussionmentioning

confidence: 99%

Cryoprotection effectiveness of low concentrations of natural and lyophilized LDL (low density lipoproteins) on canine spermatozoa

Machado‐Neves

Heneine

Henry

2014

Arq. Bras. Med. Vet. Zootec.

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The aim of this study was to evaluate the use of low concentrations of natural and lyophilized low density lipoprotein (LDL) from hen's egg yolk for cryopreservation of canine semen. Different ammonium sulphate concentrations were tested to extract LDL from egg yolk. The yolk was centrifuged, and LDL was isolated using 10, 20, 40, 45, or 50% ammonium sulphate solution (ASS). The LDL-rich floating fraction was collected for chemical characterization. Dry matter content was lowest (P<0.05) in the LDL extracted with the 50% ASS. The purification of LDL increased in association with increasing ammonium sulphate concentrations. SDS-PAGE showed that the 50% ASS solution yielded a purer fraction of LDL from egg yolk. For semen cryopreservation, TRIS extender was used replacing 20% egg yolk (control) by natural or lyophilized LDL using 1, 2, and 3% (w/v). Semen was centrifuged (755Xg for 7 min), diluted with one of the extenders, packed into 0.5mL straws (100x10 6 sperm/mL), and placed in a programmable cryopreservation machine. Thawed semen (37ºC/ 30s) was analyzed for sperm motility, morphology, and by the hypoosmotic and epifluorescence tests (CFDA/ PI). Natural LDL extracted with 50% ASS was as effective as whole egg yolk to preserve canine frozen sperm when using low concentrations. The lyophilized LDL, mainly in the two higher concentrations tested (2 and 3%), was unsuitable to maintain the effectiveness of the LDL cryoprotective effect on dog sperm.

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Structure of egg yolk very low density lipoprotein. Polydispersity of the very low density lipoprotein and the role of lipovitellenin in the structure

Evans

Bauer

Bandemer

et al. 1973

Archives of Biochemistry and Biophysics

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Influence of Feeding Cottonseed Oil to Laying Hens on the Low Density Lipoproteins of their Eggs

Cited by 12 publications

References 8 publications

The Influence of Storage of Shell Eggs Produced by Hens Fed Crude Cottonseed Oil on the Very Low Density Lipoproteins Isolated From These Eggs

The Influence of Storage of Shell Eggs Produced by Hens Fed Crude Cottonseed Oil on the Very Low Density Lipoproteins Isolated From These Eggs

Cryoprotection effectiveness of low concentrations of natural and lyophilized LDL (low density lipoproteins) on canine spermatozoa

Structure of egg yolk very low density lipoprotein. Polydispersity of the very low density lipoprotein and the role of lipovitellenin in the structure

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