Influence of fibrolytic enzymes in total mixed ration containing urea‐molasses‐treated sugarcane bagasse on the performance of lactating Holstein–Friesian crossbred cows

Abstract

The aim of this study was to determine the effect of different levels of fibrolytic enzyme on nutrient utilization and milk production in dairy cows. Four multiparous early-to-mid-lactation Holstein-Friesian crossbred cows were randomly allocated in a 4 Â 4 Latin square design. Cows were fed a balanced total mixed ration (TMR) on a dry matter (DM) basis containing 0, 1.2, 2.4, and 3.6 g/kg DM of fibrolytic enzyme in TMR, where the TMR comprises 60% concentrate supplemented with a fibrolytic enzyme at 0, 2, 4, and 6 g/kg DM of concentrate, and 40% urea-molasses-treated sugarcane bagasse (UMSB) was used as a roughage source. Fibrolytic enzyme supplementation in TMR containing UMSB did not affect dry matter intake (DMI) of dairy cows (p > 0.05). There was a quadratic effect of fibrolytic enzyme levels on the digestibility of DM, organic matter (OM), neutral detergent fiber (NDF), and acid detergent fiber (ADF) (p < 0.05), and the maximal response was reached at 1.2-2.4 g/kg DM of fibrolytic enzyme added in the TMR. Furthermore, 3.5% fatcorrected milk, milk fat, total volatile fatty acids, and propionic acid were greater in a cow fed with 1.2-2.4 g/kg DM of fibrolytic enzyme, resulting in a lower ratio of acetic acid to propionic acid (p < 0.05). In conclusion, adding a fibrolytic enzyme in TMR containing UMSB improved nutrient utilization, rumen fermentation, and milk production of lactating dairy cows.

Withdrawn: Study of the influence of exogenous fibrolytic enzyme additive on chemical composition, fermentation characteristics, and nutritional value of brewer’s spent grain

“…Besides, it increases the net energy‐lactation (NE L ) by 12%. In vivo study confirmed that EFE improves milk production (Lunsin et al., 2021 ; Mohamed et al., 2013 ). Also, at this dose, the EFE1 increased in microbial production of crude protein by 7%.…”

“…However, it decreases the potential of gas production. The inhibitory effect of EFE on high dose has also been proven in vivo (Lunsin et al, 2021) and in vitro b). This phenomenon might be explained by the fact that excessive doses of enzymes mask the surface of feed particles, which reduce the microorganisms' adhesion to the substrate and subsequent fermentation (McAllister et al (2000)).…”

“…The biological treatments are more effective than the chemical treatments due to their greater substrate specificity and their lower pollution effects (Misra et al., 2006 ). Several studies show that the use of EFE enhanced digestibility of fibrous feed (Abid et al., 2019 , 2020 ), growth performance, and milk production and economic returns in the dairy industry (Abid et al., 2020 ; Lunsin et al., 2021 ; Mohamed et al., 2013 ).…”

Effect of supplementation of exogenous fibrolytic enzymes on milk yield and composition in early lactating crossbred dairy cows

“…Statistical analysis of the data on total solids per cent revealed that there was no significant difference (p>0.05) between three treatments in any of the fortnights. In agreement with the results of the present study, Lunsin et al (2021) did not observe any effect on milk total solids content, when four levels of fibrolytic enzymes (0, 1.2, 2.4, and 3.6 g/ kg DM of TMR) having 4900 units cellulase, 18000 units of xylanase, 10000 units of betaglucanase and 17000 units amylase activities, when fed to lactating Holstein cows. Similarly, Miachieo and Thakur (2007) did not observe any effect on milk total solids content in lactating Sahiwal cows fed with wheat straw based TMR supplemented with a one : one mixture of cellulase and xylanase @ 1.5 g/kg DM and the values ranged from 13.3 to 13.4 per cent.…”

Feeding Thai Native Sheep Molasses Either Alone or in Combination with Urea-Fermented Sugarcane Bagasse: The Effects on Nutrient Digestibility, Rumen Fermentation, and Hematological Parameters

“…Regarding our data, ruminal NH 3 -N concentrations did not differ between sheep fed FSB with and without additives. This suggests that further study should focus on the optimal level of urea that can be added in order to enhance the ruminal NH 3 -N [41,42]. The proportions of acetic acid and propionic acid did not differ among treatments.…”