Influence of flumequine on in vivo mitogen responses of European eel (Anguilla anguilla L., 1758) lymphoid cells

Heijden, M.H.T. van der; Booms, G.H.R.; Tanck, Michael W.T.; Rombout, J.H.W.M.; Boon, J.H.

doi:10.1016/0165-2427(94)05397-b

Cited by 12 publications

(6 citation statements)

References 15 publications

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“…a drastic reduction of RBC, Hb and Ht, with an elevation in MCV, MCH and MCHC as compared to the not infested fish. In contrast to our findings Van Der Heijden et al (1996) determined an increase in the numbers of lymphocyte and granulocyte cells of fish infected with A. crassus.…”

Section: Discussioncontrasting

confidence: 55%

Hematological parameters associated with parasitism in pike, Esox lucius caught from Anzali wetland

Fallah

Khara

Rohi³

et al. 2013

J Parasit Dis

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This study involved 120 pike, Esox lucius, captured from Anzali wetland. Parasite fauna were identified in captured fish. Also, changes of haematological parameters were compared both infected and uninfected fish. Parasitological inspections revealed the following infestations: Skin: Lernea cyprinacea, Argulus foliaceus (Crustacean) and Tricodina sp. (Ciliatea). Gill: Dactylogyrus sp. (Digenea) and Tetraonchus monenteron (Monogenea). Eye: Diplostomum spathaceum (Digenea). Gut: Eustrongylides exises, Rhipdocotyle illense, Raphidascaris acus (Nematode), Corynosoma Strumosum (Acanthocephala). Most prevalence and intensity were related to Eustrongylides exises and Rhipdocotyle illense. Following haematological parameters were evaluated: haematocrit, haemoglobin concentration, erythrocyte and leukocyte counts, mean cell volume (MCV), mean cell haemoglobin, mean cell haemoglobin concentration lymphocytes, monocytes and eosinophils. Significant difference was found for MCV between infected and uninfected fish.

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Section: Discussioncontrasting

confidence: 55%

Hematological parameters associated with parasitism in pike, Esox lucius caught from Anzali wetland

Fallah

Khara

Rohi³

et al. 2013

J Parasit Dis

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show abstract

“…In addition effective flumequine treatment caused 200 and 100 times higher expressions of those genes, respectively. Hypothetically, this may be related to the immunomodulatory properties of those drugs [ 71 , 72 ] and in the diminished number (killed) of pathogenic A. hydrophila that can no longer depress the immune system by its virulence factors when the effective flumequine treatment was employed [ 73 , 74 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of antibiotic treatments on the expression of the R plasmid tra genes and on the host innate immune activity during pRAS1 bearing Aeromonas hydrophila infection in zebrafish (Danio rerio)

2012

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BackgroundThe transfer of R plasmids between bacteria has been well studied under laboratory conditions and the transfer frequency has been found to vary between plasmids and under various physical conditions. For the first time, we here study the expression of the selected plasmid mobility genes traD, virB11 and virD4 in the 45 kb IncU plasmid, pRAS1, conferring resistance to tetracycline, trimethoprim and sulphonamide, using an in vivo zebrafish infection- treatment model.ResultsThree days after oral infection of adult zebrafish with Aeromonas hydrophila harboring pRAS1, elevated expression of pro-inflammatory cytokine (TNF α, IL-1β and IL-8) and complement C3 genes in the intestine coincided with disease symptoms. Tetracycline, trimethoprim and an ineffective concentration of flumequine given 48 h prior to sampling, strongly increased expression of plasmid mobility genes, whereas an effective dosage of flumequine resulted in lower levels of mRNA copies of these genes relative to placebo treatment. Following effective treatment with flumequine, and ineffective treatments with a low concentration of flumequine, with trimethoprim or with sulphonamide, the intestinal expression of immune genes was strongly induced compared to placebo treated control fish.ConclusionsTreatment of zebrafish infected with an antibiotic resistant (TcR, TmR, SuR) A. hydrophila with ineffective concentrations of flumequine or the ineffective antimicrobials tetracycline and trimethoprim strongly induced expression of genes mediating conjugative transfer of the R-plasmid pRAS1. Simultaneously, there was a strong induction of selected inflammatory and immune response genes, which was again evident in fish subjected to ineffective treatment protocols. Our findings point to the essential role of therapeutic practices in escalation or control of antibiotic resistance transfer, and suggest that antibiotic substances, even in sub-inhibitory concentrations, may stimulate innate defenses against bacterial infections.

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“…Bromodeoxyuridine use in fish has also included the localization of proliferating cells in vivo [13,15]. Van der Heijden et al [14] used BrdU incorporation specifically to measure in vivo mitogen-induced lymphocyte proliferation.…”

Section: Introductionmentioning

confidence: 99%

Measurement of in vitro leucocyte mitogenesis in fish: ELISA based detection of the thymidine analogue 5′-bromo-2′-deoxyuridine

Gauthier

Cartwright

Densmore

et al. 2003

Fish & Shellfish Immunology

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Influence of flumequine on in vivo mitogen responses of European eel (Anguilla anguilla L., 1758) lymphoid cells

Cited by 12 publications

References 15 publications

Hematological parameters associated with parasitism in pike, Esox lucius caught from Anzali wetland

Hematological parameters associated with parasitism in pike, Esox lucius caught from Anzali wetland

Impact of antibiotic treatments on the expression of the R plasmid tra genes and on the host innate immune activity during pRAS1 bearing Aeromonas hydrophila infection in zebrafish (Danio rerio)

Measurement of in vitro leucocyte mitogenesis in fish: ELISA based detection of the thymidine analogue 5′-bromo-2′-deoxyuridine

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