Influence of follicular fluid GDF9 and BMP15 on embryo quality

Göde, Funda; Gülekli, Bülent; Dogan, Erbil; Korhan, Peyda; Doğan, Seda Sultan; Bige, Ozgur; Çımrın, Dilek; Atabey, Neşe

doi:10.1016/j.fertnstert.2011.03.045

Cited by 105 publications

(80 citation statements)

References 38 publications

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“…Gode et al,studied FF BMP-15 expression levels in 81 infertile women experiencing COH-ICSI-ET cycle. They did not observe significant difference in embryo quality among women regarding to BMP-15 expression status [13]. Wu et al, reported significantly increased FF BMP-15 levels in poor responders compared to that in normoresponders [14].Researchers evaluated FF BMP-15 levels of 79 couples enrolled long luteal COH-ICSI programme due to male factor infertility.…”

Section: Discussionmentioning

confidence: 99%

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

Ilhan¹,

Özkan²,

Ekinci³

et al. 2017

Histol Cytol Embryol

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Objective: Bone morphogenetic protein-15 (BMP-15) and anti-Müllerian hormone (AMH) are the proteins functioning during ovarian follicular growth. We aimed to investigate the impact of serum and follicular fluid (FF) AMH and BMP-15 levels on outcomes of intracytoplasmic sperm injection-embryo transfer (ICSI-ET) cycles.Methods: This prospective, cross-sectional study was carried out with 82 patients attending ICSI-ET cycle. The women divided into two groups according to using either GnRH agonist (group1, N=38) or antagonist (group2, N=44) for pituitary down-regulation. Eight milliliter of serum samples were taken from the patients on the 3rd day of menstrual period (D3) and on the day of oocyte pick-up (OPU); and 8 mL of follicular fluid (FF) sample was collected during OPU. AMH and BMP-15 measurements were carried with ELISA method quantitatively. Results:The mean age and body mass index of population were 30.6 ± 5.2 years and 25.5±4.2 kg/m 2 respectively. The comparison of pregnancy rates between women according to having FF AMH (35% vs 42%) and FF BMP-15 (37.8% vs 40.5%) levels under and above of the mean values revealed no significant difference. Serum AMH levels showed negative correlation with fertilization rate (FR). D3 BMP-15 level showed positive correlation with progesterone level and endometrial thickness on the day of hCG injection.Conclusions: AMH and BMP-15 monitoring during ICSI-ET cycles for predicting cycle outcomes should be evaluated with further studies.

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Section: Discussionmentioning

confidence: 99%

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

Ilhan¹,

Özkan²,

Ekinci³

et al. 2017

Histol Cytol Embryol

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“…Gode et al reported that higher levels of mature growth differentiation factor (GDF)-9 protein, a member of BMP cytokine, in the follicular fluid were significantly correlated with oocyte nuclear maturation and embryo quality [17]. There is no study regarding the relevance between clinical outcome and levels of BMP-2, -6, and 7 in follicular fluid.…”

Section: Discussionmentioning

confidence: 99%

Serum concentration of bone morphogenetic proteins (BMPs) is not linked to serum anti-mullerian hormone (AMH) level

Ogura-Nose¹,

Yokosuka²,

Akiyama³

et al. 2014

Integr Mol Med

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Serum concentration of anti-mullerian hormone (AMH) is used as a biomarker in practical clinics. As bone morphogenetic protein (BMP) cytokines induce AMH expression in human granulosa cells (GC) in vitro, serum concentrations of BMP cytokines and AMH were evaluated whether there would be a relationship between BMP cytokines and AMH. Serum concentrations of BMP-2, -6 -7, AMH and FSH were measured in 25 infertility patients using EIA or ELISA kits. Among 25 infertile patients, serum BMP-2, -6, and -7 were detected only in 10, 3, 7 patients respectively, while AMH was detected in 24 out of 25 patients. There was no relation between BMP cytokines and AMH concentration in serum. The detection rate of these BMPs in serum was much lower than that of AMH. Serum concentration of BMP-2, -6, -7 could not estimate serum AMH level.

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“…The role of MTOR in oocytes awaits further investigation. Interestingly, exogenous ODPFs augment the developmental competence of oocytes in cultured COCs (Mottershead et al, 2015;Yeo et al, 2008), and the developmental competence of double-mutant mouse oocytes and human oocytes expressing low levels of GDF9 and/or BMP15 is low (Gode et al, 2011;Su et al, 2004;Wu et al, 2007). Therefore, oocytes influence their own development through an integrated 'oocyte-granulosa cell regulatory loop', and activation of MTOR in cumulus cells is an essential step in getting this loop fully connected and functioning.…”

Section: Oocyte-dependent Activation Of Mtor Signaling In Cumulus Celmentioning

confidence: 99%

Oocyte-dependent activation of MTOR in cumulus cells controls the development and survival of cumulus–oocyte complexes

Guo¹,

Shi²,

Gong³

et al. 2016

Development

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Communication between oocytes and their companion somatic cells promotes the healthy development of ovarian follicles, which is crucial for producing oocytes that can be fertilized and are competent to support embryogenesis. However, how oocyte-derived signaling regulates these essential processes remains largely undefined. Here, we demonstrate that oocyte-derived paracrine factors, particularly GDF9 and GDF9-BMP15 heterodimer, promote the development and survival of cumulus-cell-oocyte complexes (COCs), partly by suppressing the expression of Ddit4l, a negative regulator of MTOR, and enabling the activation of MTOR signaling in cumulus cells. Cumulus cells expressed less Ddit4l mRNA and protein than mural granulosa cells, which is in striking contrast to the expression of phosphorylated RPS6 (a major downstream effector of MTOR). Knockdown of Ddit4l activated MTOR signaling in cumulus cells, whereas inhibition of MTOR in COCs compromised oocyte developmental competence and cumulus cell survival, with the latter likely to be attributable to specific changes in a subset of transcripts in the transcriptome of COCs. Therefore, oocyte suppression of Ddit4l expression allows for MTOR activation in cumulus cells, and this oocyte-dependent activation of MTOR signaling in cumulus cells controls the development and survival of COCs.

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Influence of follicular fluid GDF9 and BMP15 on embryo quality

Cited by 105 publications

References 38 publications

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

Serum concentration of bone morphogenetic proteins (BMPs) is not linked to serum anti-mullerian hormone (AMH) level

Oocyte-dependent activation of MTOR in cumulus cells controls the development and survival of cumulus–oocyte complexes

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