Influence of Gas Temperature in Atmospheric Non-Equilibrium Plasma on Bactericidal Effect

Kawano, Hiroaki; Takamatsu, Takeichiro; Matsumura, Yukihiko; Miyahara, Hidekazu; Iwasawa, Atsuo; Okino, Akitoshi

doi:10.4265/bio.23.167

Cited by 15 publications

(8 citation statements)

References 30 publications

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“…In the oxygen and carbon dioxide plasmas, the production of OH radicals, singlet oxygen, and hydrogen peroxide tended to increase as the gas temperature increased. This is similar to the report by Kawano et al on the increase in the production of the same reactive species when the gas temperature of the helium−oxygen mixture is increased [12].…”

Section: Discussionsupporting

confidence: 92%

“…To quantitatively evaluate the bactericidal effect, we used the bactericidal activity (BA) value, which can be calculated using Equation (1) [12]:…”

Section: Influence Of Plasma Gas Temperature On Bactericidal Effectmentioning

confidence: 99%

“…Although there are advantages to controlling plasma at low or room temperature to expand the range of applications, low-or room-temperature plasmas are not ideal for achieving certain processing effects. Kawano et al investigated the influence of the gas temperature of helium plasma with oxygen on the bactericidal effect of plasma, and reported that it was enhanced by increasing the gas temperature of the plasma [12].…”

Section: Introductionmentioning

confidence: 99%

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Influence of Controlling Plasma Gas Species and Temperature on Reactive Species and Bactericidal Effect of the Plasma

et al. 2021

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In this study, plasma gas species and temperature were varied to evaluate the reactive species produced and the bactericidal effect of plasma. Nitrogen, carbon dioxide, oxygen, and argon were used as the gas species, and the gas temperature of each plasma was varied from 30 to 90 °C. Singlet oxygen, OH radicals, hydrogen peroxide, and ozone generated by the plasma were trapped in a liquid, and then measured. Nitrogen plasma produced up to 172 µM of the OH radical, which was higher than that of the other plasmas. In carbon dioxide plasma, the concentration of singlet oxygen increased from 77 to 812 µM, as the plasma gas temperature increased from 30 to 90 °C. The bactericidal effect of carbon dioxide and nitrogen plasma was evaluated using bactericidal ability, which indicated the log reduction per minute. In carbon dioxide plasma, the bactericidal ability increased from 5.6 to 38.8, as the temperature of the plasma gas increased from 30 to 90 °C. Conversely, nitrogen plasma did not exhibit a high bactericidal effect. These results demonstrate that the plasma gas type and temperature have a significant influence on the reactive species produced and the bactericidal effect of plasma.

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Section: Discussionsupporting

confidence: 92%

“…To quantitatively evaluate the bactericidal effect, we used the bactericidal activity (BA) value, which can be calculated using Equation (1) [12]:…”

Section: Influence Of Plasma Gas Temperature On Bactericidal Effectmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of Controlling Plasma Gas Species and Temperature on Reactive Species and Bactericidal Effect of the Plasma

et al. 2021

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“…This plasma source was used to study the relationship between plasma gas temperature and the bactericidal effects on bacteria in liquid. 76) The plasma was generated from a helium gas with 3% oxygen admixture using a DBD plasma jet. The bactericidal effect of 80 °C plasma on E. coli was reported to be enhanced by more than 6 times compared to that of 10 °C plasma.…”

Section: Nonequilibrium Atmospheric-pressure Plasma Sources and Gas T...mentioning

confidence: 99%

Insights into normothermic treatment with direct irradiation of atmospheric pressure plasma for biological applications

Yoshimura

Otsubo

Yamashita

et al. 2020

Jpn. J. Appl. Phys.

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Characterizing the interactions between plasma and living organisms has become a multidisciplinary topic in the field of low-temperature plasma science and technology. Living organisms are hierarchically comprised of cells, tissues, organs, and organ systems. Adaptive responses are systematically controlled by the state of the surrounding environment, such as when living organisms are irradiated with discharge plasma. This review is written from a biological perspective and focuses on the importance of gas temperature control in nonequilibrium atmospheric pressure plasmas. We discuss the critical parameters associated with direct plasma treatments of living organisms. This review provides an assessment of the status of this research field and addresses the prospects for further developments in understanding the interactions between plasma and living organisms. We address the importance and necessity of normothermic plasma treatment, where an experimental system involving living organisms is controlled at an optimal temperature, which plays a significant role in plasma biology.

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“…Similarly, in the system of Cui et al (2021), argon plasma was generated by using a plasma jet that is similar shape to our plasma generating apparatus; however, the non-temperature-controlled plasma emitted from the outlet includes not only reactive species but also an electric stimulus. The types and amounts of reactive species in plasma vary depending on the generation method, gas species, and temperature (Kawano et al 2018;Takamatsu et al 2014); therefore, the differences between techniques may affect the mechanism by which macromolecules are taken up into cells.…”

mentioning

confidence: 99%

Temperature-controlled atmospheric-pressure plasma treatment induces protein uptake via clathrin-mediated endocytosis in tobacco cells

Yanagawa

Suenaga

Iijima

et al. 2022

Plant Biotechnology

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Previously, we developed a method that uses temperature-controlled atmospheric-pressure plasma to induce protein uptake in plant cells. In the present work, we examined the mechanism underlying such uptake of a fluorescenttagged protein in tobacco leaf cells. Intact leaf tissue was irradiated with N 2 plasma generated by a multi-gas plasma jet and then exposed to the test protein (histidine-tagged superfolder green fluorescence protein fused to adenylate cyclase); fluorescence intensity was then monitored over time as an index of protein uptake. Confocal microscopy revealed that protein uptake potential was retained in the leaf tissue for at least 3 h after plasma treatment. Further examination indicated that the introduced protein reached a similar amount to that after overnight incubation at approximately 5 h after irradiation. Inhibitor experiments revealed that protein uptake was significantly suppressed compared with negative controls by pretreatment with sodium azide (inhibitor of adenosine triphosphate hydrolysis) or sucrose or brefeldin A (inhibitors of clathrin-mediated endocytosis) but not by pretreatment with genistein (inhibitor of caveolae/raft-mediated endocytosis) or cytochalasin D (inhibitor of micropinocytosis/phagocytosis), indicating that the N 2 plasma treatment induced protein transportation across the plant plasma membrane via clathrin-mediated endocytosis.

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Influence of Gas Temperature in Atmospheric Non-Equilibrium Plasma on Bactericidal Effect

Cited by 15 publications

References 30 publications

Influence of Controlling Plasma Gas Species and Temperature on Reactive Species and Bactericidal Effect of the Plasma

Influence of Controlling Plasma Gas Species and Temperature on Reactive Species and Bactericidal Effect of the Plasma

Insights into normothermic treatment with direct irradiation of atmospheric pressure plasma for biological applications

Temperature-controlled atmospheric-pressure plasma treatment induces protein uptake via clathrin-mediated endocytosis in tobacco cells

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