Influence of gelatin and collagen incorporation on peroxidase-mediated injectable pectin-based hydrogel and bioactivity of fibroblasts

Abstract

Pectin has recently attracted increasing attention for biomedical and pharmaceutical applications. Due to the lack of adhesion molecules in polysaccharides, phenolic hydroxyl conjugated gelatin was added to enzymatically-gellable peroxidase-modified pectin derivative and compared with phenolic hydroxyl -pectin/collagen. Both pectin and gelatin were modified by tyramine hydrochloride in the presence of EDC/NHS. The phenolic hydroxyl -pectin/phenolic hydroxyl -gelatin, phenolic hydroxyl-pectin/collagen, and phenolic hydroxyl -pectin hydrogels were prepared using horseradish peroxidase and hydrogen peroxide. The hydrogels were characterized by gelation time analysis. Morphology, enzymatic biodegradation, mechanical and swelling properties as well as water vapor transmission rate were also evaluated. Fibroblasts were cultured for 7 days, and the survival rate was evaluated using conventional MTT assay. Hydrogels composed of Ph-pectin/Ph-gelatin showed decreased biodegradation rate, and WVTR and further improved mechanical performance in comparison with other groups. Both phenolic hydroxyl -pectin/collagen and phenolic hydroxyl -pectin/phenolic hydroxyl -gelatin hydrogels exhibited porous structures. The hydrogels composed of collagen promoted cell survival rate 1.4 and 3.5 times compared to phenolic hydroxyl -gelatin and phenolic hydroxyl -pectin based hydrogels at the end of 7 days, respectively (p < 0.001). The study demonstrated the potential of enzymatically-gellable pectin-based hydrogels as cost-effective frameworks for use in tissue engineering applications.

Phenolated alginate-collagen hydrogel induced chondrogenic capacity of human amniotic mesenchymal stem cells

“…To prepare Alg-Ph moieties, Alg was conjugated with tyramine via carbodiimide-mediated condensation in an aqueous solution. 45 The Ph contents in Alg-Ph were 1.8 mol-Ph/g-Alg. We purchased 1-Ethyl-3–(3-dimethylaminopropyl) carbodiimide (EDC), N-Hydroxysuccinimide (NHS), 2-Morpholinoethanesulfonic acid, monohydrate (MES), acetone, EtOH, and tyramine hydrochloride (Cat no: T2879) from Sigma-Aldrich.…”

Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering

“…To prepare Alg-Ph moieties, Alg was conjugated with tyramine using carbodiimide-mediated condensation [ 18 ]. To this end, we prepared 0.2% (w/v) MES and the pH value was set to 6.…”

Stepwise Optimization of Inducible Expression System for the Functional Secretion of Horseradish Peroxidase in Saccharomyces cerevisiae

“…Due to its broad-spectrum properties of oxidation, HRP has been employed in the fields of medical diagnosis, biocatalysis, bioremediation, and cancer therapy. , Especially in recent years, HRP was developed as a powerful food additive for the cross-linking of proteins or biopolymers − and bacteriostasis . Besides these applications, HRP has the potential to be used in the production of a biocompatible scaffold (hydrogels) for tissue engineering , and an edible microscale scaffold (gelatin and collagen) for promising cell-based artificial meat . Thus, with increasing applications in food manufacturing, more and more food-grade HRP is required.…”

“…7 Besides these applications, HRP has the potential to be used in the production of a biocompatible scaffold (hydrogels) for tissue engineering 8,9 and an edible microscale scaffold (gelatin and collagen) for promising cell-based artificial meat. 10 Thus, with increasing applications in food manufacturing, more and more food-grade HRP is required.…”