Influence of Gestational Age, Cesarean Section and Hematocrit on IL-8 Concentrations in Plasma and Detergent-Lysed Whole Blood of Noninfected Newborns

Neunhoeffer, Felix; Lipponer, Diana; Eichner, Martin; Poets, Christian F.; Wacker, A.; Orlikowsky, Thorsten

doi:10.1159/000328860

Cited by 2 publications

(3 citation statements)

References 29 publications

(38 reference statements)

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“…We observed lower hemoglobin levels to be associated with higher IL-8 levels in plasma samples, especially after surgery. Previous studies suggested that measurements of plasma IL-8 could be affected by hematocrit ( Neunhoeffer et al., 2011 ). The stronger relation observed on the postoperative day may be a statistical phenomenon, explained by the higher variability of IL-8 and hemoglobin levels after surgery.…”

Section: Discussionmentioning

confidence: 99%

“…Preoperative anemia and intraoperative blood loss are known to increase the risk for POD and IL-8 binding could be one factor by which anemia facilitates delirium ( Joosten et al., 2006 ; Marcantonio et al., 1998 ). One previous study suggested hematocrit to affect plasma IL-8 measurements ( Neunhoeffer et al., 2011 ). Perioperative anemia could be a relevant confounding factor in studies on IL-8 and delirium.…”

Section: Introductionmentioning

confidence: 99%

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Interleukin 8 in postoperative delirium – Preliminary findings from two studies

Lammers-Lietz

Akyüz

Feinkohl

et al. 2022

Brain, Behavior, & Immunity - Health

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Objective Studies have suggested that inflammation contributes to the pathogenesis of postoperative delirium, but previous results on the proinflammatory cytokine IL-8 in plasma are contradictory. Additionally, a significant fraction of IL-8 is bound to erythrocytes, but the relevance of whole blood IL-8 in delirium has not been studied. In this work, we analyzed the association of postoperative delirium with levels of unbound IL-8 in plasma and levels of IL-8 in whole blood in patients from two studies which were conducted in our department and have not been presented previously. We assessed the prognostic value of whole blood IL-8. Methods Plasma/whole blood IL-8 was measured at least once in N = 504 patients preoperatively, on day one (d1) and/or three months after surgery in the BioCog observational study. Whole blood IL-8 was measured in N = 64 patients from the PHYDELIO trial preoperatively, on d1 and d7 after surgery. For the determination of whole blood IL-8, EDTA-preserved blood samples underwent lysis by adding Triton-X100 surfactant. Plasma and whole blood IL-8 levels were assessed with two different immunoassay kits. Delirium was appraised systematically for seven postoperative days according to DSM criteria using two comparable protocols consisting of validated screening tools. Results Delirium occurred in 25% of BioCog and 14% of PHYDELIO patients. In BioCog, IL-8 was elevated on d1 and in delirious patients. A steeper postoperative increase in delirium was confounded by surgery-related factors. A crescendo-decrescendo pattern of whole blood IL-8 levels was observed in non-delirious patients with a peak on d1. This pattern was more distinct in delirious BioCog patients, but inverted in delirious PHYDELIO patients. Preoperative whole blood IL-8>318.4 pg/mL (reference <150 pg/mL) had adequate sensitivity (0.79/0.78) and specificity (0.53/0.67) for delirium in both samples. Conclusion Our results contribute to an inflammatory hypothesis of postoperative delirium.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interleukin 8 in postoperative delirium – Preliminary findings from two studies

Lammers-Lietz

Akyüz

Feinkohl

et al. 2022

Brain, Behavior, & Immunity - Health

View full text Add to dashboard Cite

show abstract

“…IL-6 and IL-8 increase rapidly after exposure to bacterial products [20]. Interleukins have a short half-life, have to be processed and analyzed promptly and their value depends on a variety of characteristics which have to be considered [29]. Therefore it should be used in conjunction with a 'late' and more specific marker such as CRP to improve its diagnostic capability in clinical practice [31].…”

mentioning

confidence: 99%