Influence of Gold Nanoparticles on Human Fibroblast Before and After Cryopreservation

Pavlovich, E. V.; Волкова, Н. А.

doi:10.1007/978-3-319-18543-9_28

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…Based on the results of our previous studies, by using confocal laser microscopy of bone marrow MSC culture preparations, it has been shown that low-frequency gold does not remain on the plasma membrane of cells. The localization was partially observed in the cytoplasm and in the cell nucleus [23,24]. It can be assumed that the AuNPs, by binding to the nucleus cell membrane, have the potential Control Au_NPs_1.5 g/ml Au_NPs_3 g/ml…”

Section: Effect Of Aunps On Synthesis Collagen I Type Of Mscsmentioning

confidence: 99%

Studies of the Influence of Gold Nanoparticles on Characteristics of Mesenchymal Stem Cells

Волкова

Pavlovich

Fesenko

et al. 2017

Journal of Nanomaterials

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The aim of the present study is to determine what effect the different concentrations of 15 nm gold nanoparticles (AuNPs) will have on the immunophenotype, synthesis collagen type I, ability to direct differentiation and spectroscopic characteristics of bone marrow mesenchymal stem cells (MSCs). The AuNPs in concentrations of 1.5-9 g/ml did not lead to changes in the level of expression of CD 45, CD 90, and CD 73. It should be noted that AuNPs in concentrations of 6 and 9 g/ml led to a decrease in CD 44 cells by 6% and 9%, respectively. The content of CD 105 cells was reduced by 5% when AuNPs were applied at a concentration of 9 g/ml. It was found that AuNPs in concentrations of 1.5-6 g/ml are safe for MSCs, while the increase up to 9 g/ml has a toxic effect, manifested by the reduction of synthesis collagen type I and ability of adipogenic differentiation. IR spectroscopy data have shown that the AuNPs at concentrations of 9 g/ml under conditions of adipogenic differentiation to MSCs lead to the destruction processes in the cells. The obtained results are related to the field of applied nanotechnology, which extends to regenerative medicine, especially in development of bioimplantology.

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Section: Effect Of Aunps On Synthesis Collagen I Type Of Mscsmentioning

confidence: 99%

Studies of the Influence of Gold Nanoparticles on Characteristics of Mesenchymal Stem Cells

Волкова

Pavlovich

Fesenko

et al. 2017

Journal of Nanomaterials

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“…Various fullerene derivatives have been claimed to reduce LPO and acrosomal damage and to increase antioxidant enzyme activities and motility in pig (at 4°C for 10 days, Li et al., 2019) and goat (at 32°C for 3 h, Murugan et al., 2002) semen stored for a short time. Besides, it was found that 10 and 20 μg/mL of fullerene derivatives prevented motility losses caused by freeze–thawing procedure in humans, whereas the concentrations of 40 μg/mL and above decreased motility and membrane functionality (Pavlovich, 2014). Based on the results of our previous study (Güngör et al., 2022), 200, 400, 800 nM, 1 and 5 μM doses of C 60 HyFn were used in this study.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that cryopreservation damages sperm DNA during cold storage (moderately) and after freeze–thawing (severely), and that these damages are positively associated with motility losses (Lopez‐Fernandez et al., 2008). In a study, it has been alleged that 10 and 20 μg/mL of C 60 HyFn concentrations had no effect on the DNA of frozen–thawed human sperm, but 40 μg/mL and/or above concentrations are harmful (Pavlovich, 2014). On the other hand, it is emphasized that C 60 HyFn reduces apoptosis by inactivating caspases, whereas C 60 dissolved in tetrahydrofuran solution increases apoptosis by activating caspases, so the effects of C 60 solvents on apoptosis are very important (Harhaji et al., 2008).…”

Section: Discussionmentioning

confidence: 99%

Reduction of cryopreservation‐induced structural, functional and molecular damages in ram sperm by hydrated C₆₀ fullerene

Güngör,

Türk,

Dayan Cinkara

et al. 2023

Reprod Domestic Animals

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This study aimed to investigate the morphological, functional and molecular changes in frozen–thawed ram sperm using an extender containing different concentrations of hydrated carbon 60 fullerene (C60HyFn), a nanotechnological product. Semen taken from each of the seven Akkaraman rams were pooled. Semen collection was done twice a week and it continued for 3 weeks. Each pooled semen sample was divided into six equal groups and diluted with tris + egg yolk extender including 0 (control), 200, 400, 800 nM, 1 and 5 μM concentrations of C60HyFn at 37°C. They were then frozen in liquid nitrogen vapour at −140°C, stored in liquid nitrogen container (−196°C) and thawed at 37°C for 25 s before analysis. In comparison with control, C60HyFn addition prior to freezing procedure provided significant increases in total and progressive motility rates, glutathione peroxidase, catalase activities and percentage of highly active mitochondria, and significant decreases in dead and abnormal sperm rates, lipid peroxidation, caspase‐3 and DNA fragmentation levels in frozen–thawed ram semen. When compared to control, C60HyFn supplementation significantly down‐regulated the expression levels of miR‐200a and KCNJ11, and significantly up‐regulated the expression levels of miR‐3958‐3p (at the concentrations of 200, 400, 800 nM and 1 μM), CatSper1 (at the concentrations of 200, 400 nM and 5 μM), CatSper2 (at the concentrations of 1 and 5 μM), CatSper3 (at the concentrations of 200, 400 nM, 1 and 5 μM), CatSper4 (at all concentrations), ANO1 (at the concentrations of 800 nM, 1 and 5 μM) and TRPV5 (at the concentrations of 200, 400 and 800 nM). The addition of C60HyFn had no effect on global DNA methylation rates. As a result, C60HyFn supplementation to ram semen extenders may be beneficial in reducing some of the functional, structural and molecular damages in sperm induced by the freeze–thawing procedure.

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“…In some reports, there are contradictory data about AuNP application during cell culturing both in monolayer and suspension. Early, we studied the viability, proliferative capacity, and apoptosis/necrosis in human fibroblast culture (HFC) prior to and after cryopreservation in the presence of gold nanoparticles [ 9 ]. The use of AuNPs under low concentrations resulted in an increased proliferative activity of HFC with no activation of apoptosis and necrosis.…”

Section: Introductionmentioning

confidence: 99%

Application of Cryopreserved Fibroblast Culture with Au Nanoparticles to Treat Burns

Волкова¹,

Yukhta²,

Pavlovich³

et al. 2016

Nanoscale Res Lett

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The aim was to investigate a possibility of using the cryopreserved human culture of fibroblasts (CrHFC) with gold nanoparticles (AuNPs) to treat experimental burns in rats.The third-degree burns were modeled in white male rats. All the animals with burns were divided into three experimental groups: control group with no wound treatment; group 1 was composed of animals with CrHFC application; and group 2 consisted of those with CrHFC and AuNPs (6 μg/ml) application to a burn surface the next day after the injury. The CrHFC was applied to the methylcellulose gel in a dose of 5 × 104 of viable cells per 1 cm2 of the burn. The animals were removed from the experiment on day 21 after the treatment.The CrHFC use alone and with AuNPs to the surface of burns stimulated the wound healing compared with the control. The effect of using CrHFC was less pronounced compared to the CrHFC application with AuNPs. It was reflected in a slower recovery of burns and moderate lymphocytic infiltration of granulation tissue. Immunofluorescent analysis emphasized that the use of CrHFC with AuNPs accelerated the skin synthetic processes and was helpful in recovering type I and III collagen content on day 21 after therapy.The results were likely related primarily to the unique structure and antimicrobial properties of AuNPs. Our experimental study of the effect of CrHFC with AuNPs application on regenerative processes in burns gives some pre-conditions to the following advanced bio- and nanotechnology developments.

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Influence of Gold Nanoparticles on Human Fibroblast Before and After Cryopreservation

Cited by 5 publications

References 15 publications

Studies of the Influence of Gold Nanoparticles on Characteristics of Mesenchymal Stem Cells

Studies of the Influence of Gold Nanoparticles on Characteristics of Mesenchymal Stem Cells

Reduction of cryopreservation‐induced structural, functional and molecular damages in ram sperm by hydrated C₆₀ fullerene

Application of Cryopreserved Fibroblast Culture with Au Nanoparticles to Treat Burns

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Influence of Gold Nanoparticles on Human Fibroblast Before and After Cryopreservation

Cited by 5 publications

References 15 publications

Studies of the Influence of Gold Nanoparticles on Characteristics of Mesenchymal Stem Cells

Studies of the Influence of Gold Nanoparticles on Characteristics of Mesenchymal Stem Cells

Reduction of cryopreservation‐induced structural, functional and molecular damages in ram sperm by hydrated C60 fullerene

Application of Cryopreserved Fibroblast Culture with Au Nanoparticles to Treat Burns

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Reduction of cryopreservation‐induced structural, functional and molecular damages in ram sperm by hydrated C₆₀ fullerene