Studies of the Influence of Gold Nanoparticles on Characteristics of Mesenchymal Stem Cells

Abstract

The aim of the present study is to determine what effect the different concentrations of 15 nm gold nanoparticles (AuNPs) will have on the immunophenotype, synthesis collagen type I, ability to direct differentiation and spectroscopic characteristics of bone marrow mesenchymal stem cells (MSCs). The AuNPs in concentrations of 1.5-9 g/ml did not lead to changes in the level of expression of CD 45, CD 90, and CD 73. It should be noted that AuNPs in concentrations of 6 and 9 g/ml led to a decrease in CD 44 cells by 6% and 9%, respectively. The content of CD 105 cells was reduced by 5% when AuNPs were applied at a concentration of 9 g/ml. It was found that AuNPs in concentrations of 1.5-6 g/ml are safe for MSCs, while the increase up to 9 g/ml has a toxic effect, manifested by the reduction of synthesis collagen type I and ability of adipogenic differentiation. IR spectroscopy data have shown that the AuNPs at concentrations of 9 g/ml under conditions of adipogenic differentiation to MSCs lead to the destruction processes in the cells. The obtained results are related to the field of applied nanotechnology, which extends to regenerative medicine, especially in development of bioimplantology.

Synchrotron-based infrared microspectroscopy study on the radiosensitization effects of Gd nanoparticles at megavoltage radiation energies

“…Previous studies have shown that NP can induce conformational changes in cell proteins. [36][37][38] Moreover, Taupin et al reported proliferation arrest and cell cycle modifications when using similar NP to those used in our work. 19 Thus, these FTIRM spectra changes could also be related to initial modifications in the cells induced by the NP.…”

Green Synthesis of Gold Nanoparticles Using Upland Cress and Their Biochemical Characterization and Assessment

“…A cell viability of ~79% for BMSCs was reported with spherical Au NPs (18 nm; 0.09 mM) [ 44 ] which is comparable to our results as well (77%; 0.1 mM). A recent study indicated that 15 nm Au NPs could affect the characteristic MSC marker expression (e.g., CD 105) and cell differentiation, especially when the concentration is higher than 9 µg/mL [ 45 ]. Cell damage and apoptosis may be explained by the generation of reactive oxidative stresses (ROS) [ 46 ].…”

Transplantation of Gold Nanoparticles With or Without Bone Marrow Stem Cells for Regeneration of Surgically Induced Tongue Defect in Rats

“…GNPs are adopted by MSCs causing increase in their migration rateto osteoblast cells over adipocyte cells throughencouragingosteogenic transcriptional outline. Volkova N et al 2017 (26) In vivo…”