Influence of grafting conditions on the properties of polyacrylamide-based cation-exchange cryogels grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid

Chen, Fang; Yao, Kangde; Shen, Shihua; Yun, Junxian

doi:10.1016/j.ces.2007.09.008

Cited by 27 publications

(4 citation statements)

References 27 publications

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“…All in all, the permeability values observed are well compatible with liquid chromatography applications. For example, Chen et al reported the permeability values that are almost in the same range as the ones reported here while Yao et. al .…”

Section: Resultssupporting

confidence: 85%

“…However, highly porous structures are also limited in the total internal area they can offer to bind such products and thus, binding capacity values are typically low. The creation of composite materials by polymer grafting may provide a solution by introducing polymer “brushes” . Our group has recently showed the viability of utilizing radiation‐induced grafting techniques .…”

Section: Resultsmentioning

confidence: 99%

“…(O) represent MP‐CM, (□) are 105% MP‐EP, (Δ) shows MP‐S, (▿) represents MP‐DE. The values inside the circle are from reference Yao et al (lower flow rate) and Chen et al (higher flow rate) for comparison.…”

Section: Resultsmentioning

confidence: 99%

“…Conversely, Ventura et al achieved a very high binding capacity (360 mg/g) for hollow‐fiber membranes at 20% grafting density. Similarly, other authors employed macroporous substrates that led to adsorbent systems with moderate protein binding properties . In a previous paper, we have shown that megaporous structures can be upgraded, via different methods, with a high degree of grafting (53%) and that this procedure actually produces a composite material with increased protein binding characteristics (108 mg/g).…”

Section: Introductionmentioning

confidence: 86%

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Grafted megaporous materials as ion‐exchangers for bioproduct adsorption

Bibi

Fernández‐Lahore

2013

Biotechnology Progress

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Megaporous chromatographic materials were manufactured by a three-step procedure, including backbone synthesis, chemical grafting, and introduction of ion-exchange functionality. The backbone of the adsorbent cylindrical bodies was prepared by polymerization of methacrylic acid and poly(ethylene glycol) diacrylate at sub-zero temperatures. Grafting was performed employing glycidyl methacrylate and a chemical initiator, cerium ammonium nitrate. The degree of grafting was adjusted by modifying the concentration of the initiator in the reaction mixture to a range of values (23, 39, 62, 89, and 105%). Further, the pendant epoxy-groups generated by the previous step were reacted to cation- and anion-exchanging moieties utilizing known chemical routes. Infrared spectroscopy studies confirmed the incorporation of epoxy and ion-exchanger groups to the backbone material. Optimized materials were tested for chromatography applications with model proteins; the dynamic binding capacity, as recorded at 10% breakthrough and 2.0 × 10(-4) m/s superficial velocity, were 350 and 58 mg/g for the cation-exchanger and the anion-exchanger material, respectively. These results may indicate that long tentacle-type polymer brushes were formed during grafting therefore increasing the ability of the megaporous body to efficiently capture macromolecules.

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 86%

See 2 more Smart Citations

Grafted megaporous materials as ion‐exchangers for bioproduct adsorption

Bibi

Fernández‐Lahore

2013

Biotechnology Progress

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Latest Advances in Cryogel Technology for Biomedical Applications

Memić

Colombani

Eggermont

et al. 2019

Advanced Therapeutics

234

241

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There exists a technological need for advanced materials with improved properties for emerging biomedical applications. Recent developments in macroporous materials have demonstrated their applicability as indispensable tools in biomedical research. Cryogels, which are materials with a macroporous 3D structure, are produced as a result of controlled freezing during polymerization with a highly interconnected polymer network. Cryogels’ interest lies in their ability to address some of the limitations of their hydrogel analogues. In this review, hydrogel and cryogel basic concepts are discussed as a short primer for readers unfamiliar with the cryogels literature. Next, a general overview of the methods for synthesis and characterization of cryogels is provided, highlighting key concepts relevant to cryogels and explaining their unique properties. Finally an in‐depth overview of specific technologies and fields where cryogels have been applied is given. It is argued that the latest advances in cryogel technologies are able to address challenges in bioseparation, tissue engineering, and other emerging bioengineering disciplines.

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Synthesis and performance of 3D‐Megaporous structures for enzyme immobilization and protein capture

Bibi

Gavara

Espinosa

et al. 2011

Biotechnology Progress

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The preparation of megaporous bodies, with potential applications in biotechnology, was attempted by following several strategies. As a first step, naive and robust scaffolds were produced by polymerization of selected monomers in the presence of a highly soluble cross-linker agent. Ion-exchange function was incorporated by particle embedding, direct chemical synthesis, or radiation-induced grafting. The total ionic capacity of such systems was 1.5 mmol H(+)/g, 1.4 mmol H(+)/g, and 17 mmol H(+)/g, respectively. These values were in agreement with the ability to bind model proteins: observed dynamic binding capacity at 50% breakthrough was ≅7.2 mg bovine serum albumin/g, ≅7.4 hen egg-white lysozyme (HEWL) mg/g, and ≅108 HEWL mg/g. In the later case, total (static) binding capacity reached 220 mg/g. It was observed that the structure and size of the megapores remained unaffected by the grafting procedure which, however, allowed for the highest protein binding capacity. Lysozyme supported on grafted body showed extensive clarification activity against a Micrococcus lysodekticus suspension in the flow-through mode, i.e., 90% destruction of suspended microbial cells was obtained with a residence time ≈ 18 min. Both protein capture and biocatalysis applications are conceivable with the 3D-megaporous materials described in this work.

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Influence of grafting conditions on the properties of polyacrylamide-based cation-exchange cryogels grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid

Cited by 27 publications

References 27 publications

Grafted megaporous materials as ion‐exchangers for bioproduct adsorption

Grafted megaporous materials as ion‐exchangers for bioproduct adsorption

Latest Advances in Cryogel Technology for Biomedical Applications

Synthesis and performance of 3D‐Megaporous structures for enzyme immobilization and protein capture

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