Influence of growth phase on bacterial cell electrokinetic characteristics examined by soft particle electrophoresis theory

Experimental Study and ANN Dual-Time Scale Perturbation Model of Electrokinetic Properties of Microbiota

“…The solution (5 mL) was conducted to carry out the electrokinetic properties of RMMs assay. The electrophoretic mobility (μ e ) of microbial cells was measured according to a phase amplitude light scattering (PALS) described by Hayashi et al (Hayashi et al, 2003) and Eboigbodin et al (de Kerchove and Elimelech, 2005), using the Zeta Potential Analyzer (NanoBrook ZetaPALS, Brookhaven Instruments Corp., Holtsville, NY, USA) (Instruments, 2012) equipped with a He-Ne laser (658.0 nm) as a source of light in a high precision system (30 cycles) at 39°C. The ξ was calculated according to equation (1) by the Zeta potential analyzer.…”

Soft-particle model analysis of effect of LPS on electrophoretic softness of Acidithiobacillus ferrooxidans grown in presence of different metal ions

“…The fixed charge density ( fix ) of the wild strain was −1.142 × 10 4 C/m 3 . Fixed charge density and electrophoretic softness were found to differ widely for various strains depending on the bacterial surface structure and culture conditions [7,16]. Literature reported values of fixed charge density are in the order of 10 4 -10 6 C/m 3 [17][18][19].…”

Significance of cell electrokinetic properties determined by soft-particle analysis in bacterial adhesion onto a solid surface

“…Ohshima's softparticle electrophoresis theory applies to biological cells with ion-penetrable layers around core particles [5][6][7]. This theory was applied in analyzing bacterial electrophoresis data [8][9][10][11]. Sonohara et al [12] distinguished differences in electrokinetic properties between gram-positive and gramnegative strains using this theory.…”