Soft-particle model analysis of effect of LPS on electrophoretic softness of Acidithiobacillus ferrooxidans grown in presence of different metal ions

Chandraprabha, M.N.; Modak, Jayant M.; Natarajan, K.A.

doi:10.1016/j.colsurfb.2008.10.006

Cited by 13 publications

(13 citation statements)

References 20 publications

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“…Here, it should be noted that the λ -1 does not mean the length of polymeric layers; instead, the value is related to the extent of the ion-permeable layer. 45 In addition, the corresponding F fix values were found to be -0.058 and -0.446 mM (-5.6 × 10 79 The differences in λ -1 and F fix for cells after the enzyme treatment clearly show that the thickness and charged nature of the outside polymeric layer has changed. This implies that considerable length of the extracellular polymeric layer was changed at the outer cell surface.…”

Section: Influence Of Extracellular Macromolecules On Surface Propertmentioning

confidence: 96%

Surface Characteristics and Adhesion Behavior of Escherichia coli O157:H7: Role of Extracellular Macromolecules

et al. 2009

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Experiments were conducted using enterohemorrhagic Escherichia coli O157:H7 cells to investigate the influence of extracellular macromolecules on cell surface properties and adhesion behavior to quartz sand. Partial removal of the extracellular macromolecules on cells by a proteolytic enzyme (proteinase K) was confirmed using Fourier transform infrared spectroscopy analyses. The proteinase K treated cells exhibited more negative electrophoretic mobility (EPM) at an ionic strength (IS) e 1 mM, a slightly lower isoelectric point, and were less hydrophobic as compared to the untreated cells. Potentiometric titration results indicated that the total site concentration (i.e., the total amount of exposed functional groups per cell) on the treated cells was approximately 22% smaller than the untreated cells, while the dissociation constants were almost identical. Analysis of the EPM data using soft particle theory showed that the removal of extracellular macromolecules resulted in polymeric layers outside the cell surface that were less electrophoretically soft. The more negative mobility for the treated cells was likely due to the combined effects of a change in the distribution of functional groups and an increase in the charges per unit volume after enzyme treatment and not just removal of extracellular macromolecules. The proteolytic digestion of extracellular macromolecules led to a significant difference in the cell adhesion to quartz sand. The adhesion behavior for treated cells was consistent with DLVO theory and increased with IS due to less negativity in the EPM. In contrast, the adhesion behavior of untreated cells was much more complex and exhibited a maximum at IS ) 1 mM. The treated cells exhibited less adhesion than the untreated cells when the IS e 1 mM due to their more negative EPM. However, when the IS g 10 mM, a sudden decrease in the removal efficiency was observed only for the untreated cells even through EPM values were similar for both treated and untreated cells. This result suggested that an additional non-DLVO type interaction, electrosteric repulsion, occurred at higher IS (g10 mM in this study) for the untreated cells due to the presence of extracellular macromolecules that hindered cell adhesion to the quartz surface. This finding provides important insight into the role of macromolecule-induced E. coli O157:H7 interactions in aquatic environments.

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Section: Influence Of Extracellular Macromolecules On Surface Propertmentioning

confidence: 96%

Surface Characteristics and Adhesion Behavior of Escherichia coli O157:H7: Role of Extracellular Macromolecules

et al. 2009

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“…A so charged interface model and the related electrostatics and electrokinetics have oen been used to model a number of biological and non-biological phenomena. [1][2][3][4][5][6][7] Some well-known examples include quantifying electrophoretic properties of cells, bacteria and viruses, [8][9][10][11][12][13][14][15][16] studying bacterial adhesion to surfaces, [17][18][19] interpreting agglutination and shape transition of red blood cells, 20,21 specifying properties of non-biological so charged gels and lms, [22][23][24][25] enhancing electrochemomechanical energy conversion efficiency in polymer-graed nanochannels, 26 designing so-charged-interface-based double layer capacitors, 27 and many more.…”

Section: Introductionmentioning

confidence: 99%

Electrostatics of soft charged interfaces with pH-dependent charge density: effect of consideration of appropriate hydrogen ion concentration distribution

Chen

Das

2015

RSC Adv.

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“…A more hydrophilic bacterial community persists and therefore may inhibit aggregation (Gaboriaud et al, 2006). Conversely, increased ionic concentration of the environmental solution may cause the polymer layer to decrease in thickness, thereby concentrating overall charge density at the bacterial surface, increasing electrostatic repulsion, and reducing aggregation (Chandraprabha et al, 2009). For purposes of this study, we examined the overall effect of increased organic matter, which increases microbial activity (therefore EPS) and contributes to biofilm accumulation and potentially increased aggregation.…”

Section: Importance Of Aggregation and Compaction In The Environmentmentioning

confidence: 97%

Biotic and abiotic controls on sediment aggregation and consolidation: Implications for geochemical fluxes and coastal restoration

Land

Kolker

Gambrell

2012

Marine Environmental Research

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Soft-particle model analysis of effect of LPS on electrophoretic softness of Acidithiobacillus ferrooxidans grown in presence of different metal ions

Cited by 13 publications

References 20 publications

Surface Characteristics and Adhesion Behavior of Escherichia coli O157:H7: Role of Extracellular Macromolecules

Surface Characteristics and Adhesion Behavior of Escherichia coli O157:H7: Role of Extracellular Macromolecules

Electrostatics of soft charged interfaces with pH-dependent charge density: effect of consideration of appropriate hydrogen ion concentration distribution

Biotic and abiotic controls on sediment aggregation and consolidation: Implications for geochemical fluxes and coastal restoration

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