Influence of handling procedures on rat plasma creatine kinase activity

Yerroum, Mohammed; Braconnier, F; Chariot, Patrick

doi:10.1002/(sici)1097-4598(199908)22:8<1119::aid-mus16>3.0.co;2-s

Cited by 16 publications

(13 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the same way, variations of the CK activities in the plasma of controls in the present study would be induced by the emotional stress due to handling and probably by the additional stress of the venipuncture. YERROUM et al (24) reported that CK values were higher 564 U/L in rats held behind the front legs compared to control group (272 U/L) (p < 0.01). The physiological significance of the observed changes in plasma CK activity during acute inflammation could be associated with increased production of some positive acute phase proteins such as haptoglobin, fibrinogen, ceruloplasmin during prolonged inflammation, as shown in weaning rabbits with E. coli infection (25), and C-reactive protein in rabbits treated for 26 days twice weekly with turpentine (26).…”

Section: Discussionmentioning

confidence: 94%

Effect of Staphylococcus Aureus infection on plasma creatine kinase activity in rabbits

Georgieva¹,

Petrov²,

Lyutskanov³

et al. 2016

TJS

View full text Add to dashboard Cite

The aim of the present study was to investigate the variations of the plasma creatine kinase (CK) activities following an experimentally induced staphylococcal infection in rabbits and to compare them with the occurrence of clinical signs. For that, the plasma CK activities were determined in 7 male New Zealand White, 3 months old rabbits subcutaneously injected with Staphylococcus aureus strain suspension (density: 8х10 8 cfu/mL) and in 6 other rabbits not treated (controls). In parallel, rectal temperature and skin lesions (abscesses) were recorded. In all infected animals, formation of abscess due to the proliferation of the inoculated strain was observed within 48-96 hours following the bacterial inoculation and these lesions have gradually extended leading to purulent exudates several days after and sometimes to secondary abscesses in surrounding muscles. The concentration of fibrinogen was used as an acute phase protein with aim to confirm the incidence of infection. Plasma CK activities were dramatically elevated on 48 hour compared to initial values and to controls at the same time but this increase was transient, the CK activities being similar to controls since the 72 nd hour. These results indicate that the plasma CK activity in rabbit can be considered as a precocious marker of skin and secondary muscular damage related to the abscess formation and probably to the bacterial exotoxins following experimental staphylococcal infection.

show abstract

Section: Discussionmentioning

confidence: 94%

Effect of Staphylococcus Aureus infection on plasma creatine kinase activity in rabbits

Georgieva¹,

Petrov²,

Lyutskanov³

et al. 2016

TJS

View full text Add to dashboard Cite

show abstract

“…Blood cholesterol, triglyceride, and CK levels of the control rats (Table 2) were in the normal range (Suckow et al 2006, Yerroum et al 1999. On the other hand, as a result of the diet a marked, slightly more then seven times rise (from 1.45 to 10.7) in the total cholesterol level was observed in HC rats (Table 2).…”

Section: Blood Testsmentioning

confidence: 91%

Fluvastatin-induced alterations of skeletal muscle function in hypercholesterolaemic rats

Füzi

Palicz

Vincze

et al. 2011

J Muscle Res Cell Motil

View full text Add to dashboard Cite

Although statins, the most widely used drugs in the treatment of hyperlipidaemia, are generally accepted as efficient and safe drugs their side-effects on skeletal muscle have been reported with increasing frequency in the past years. The lack of an appropriate animal model in which these side effects would consistently be observed is one of the most important drawbacks in studying statin associated myopathy. To overcome this and enable the studying of the effects of fluvastatin on skeletal muscles an animal model with high blood cholesterol levels was developed. In these animals cholesterol levels rose more than seven fold (from soleus the duration of twitch and tetanic force was shortened. These results clearly indicate that statin administration in these animals results in a myopathy characterized by decreased muscle force and elevated plasma creatine kinase level.

show abstract

“…Stress by habituation (Goicoechea et al, 2008), handling (Goicoechea et al, 2008;Kawahara et al, 1999;Yerroum et al, 1999), and restraint (Tabata et al, 1998;Vahl et al, 2005) has been suggested to contribute to large variability of various biomarkers such as CK. Even though rats in our study were accustomed to laboratory handling procedures for more than one week to reduce the variability due to the habituation and handling procedures according to the report by Goicoechea et al (2008), CK collected by C-JV exhibited relatively higher variability than that collected by A-JV.…”

Section: Discussionmentioning

confidence: 99%

“…However, techniques for blood sampling from small laboratory animals such as rats have different impacts on various factors due to differences in habituation (Goicoechea et al, 2008), handling (Goicoechea et al, 2008;Kawahara et al, 1999;Yerroum et al, 1999), restraint (Tabata et al, 1998;Vahl et al, 2005), bleeding sites (van Herck et al, 2001;Matsuzawa et al, 1993;Conybeare et al, 1988), or anesthesia (Altholtz et al, 2006;Arnold and Langhans, 2010;Fitzner et al, 2006). Consequently, the different impacts of blood sampling techniques on blood analysis may introduce confounding variables in data interpretation.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the fluctuation of these isoenzymes in rats must be interpreted with caution, depending upon the factors which influence both the total and isoenzyme-specific activities. Furthermore, unlike AST, CK has been reported to vary from 100 U/L to 1,300 U/L even in naive rats due to different sampling techniques aforementioned (Goicoechea et al, 2008;Yerroum et al, 1999;Matsuzawa et al, 1993). However, in the majority of toxicological studies, analytical specifications and recommendations are described either poorly or not at all, making it difficult to standardize them for non-terminal repetitive blood sampling (Goicoechea et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of different blood sampling techniques on plasma biomarkers for skeletal myopathy in conscious rats

Miwa

Tamai

Kinpara

et al. 2015

Fundam. Toxicol. Sci.

View full text Add to dashboard Cite

-To characterize variability of various musculoskeletal biomarkers by different blood sampling techniques in conscious rats, plasma asparate aminotransferase (AST), creatine kinase (CK) and its isoenzymes, fatty acid binding protein 3 (FABP3), myosin light chain 3 (Myl3) and microRNA (miR-133a) obtained by jugular venipuncture (C-JV) or tail venipuncture (C-TV) were compared with those obtained by jugular venipuncture (A-JV) in isoflurane-anesthetized rats. Plasma CK, FABP3 and Myl3, especially when collected by C-TV, were higher with larger variability than when collected by A-JV, whereas miR-133a displayed large variability in all techniques. Interestingly, higher CK obtained by C-JV or C-TV was largely attributable to higher CK-MM or CK-BB, respectively. Handling and restraint stress were identified as possible factors contributing to larger variability for CK, FABP3 and Myl3. A close correlation between CK and FABP3 was demonstrated both in the C-JV and C-TV techniques. Next, we evaluated the impact of C-JV and C-TV techniques for detecting skeletal myopathy in 2,3,5,6-tetramethyl-p-phenylenediamine-treated rats. In this model, CK and CK-MM obtained by C-TV were significantly increased, but those obtained by C-JV were not modified. In contrast, AST, FABP3, Myl3 and miR-133a obtained by both techniques were drastically elevated to a similar extent. The results suggest that, in conscious rats, the tail venipuncture technique may be more appropriate to detect skeletal myopathy despite the higher variability with this technique than with the jugular venipuncture technique. Furthermore, FABP3, Myl3 and miR-133a may serve as more sensitive biomarkers with large signal-to-noise ratios regardless of the blood sampling technique in conscious rats.

show abstract

Influence of handling procedures on rat plasma creatine kinase activity

Cited by 16 publications

References 14 publications

Effect of Staphylococcus Aureus infection on plasma creatine kinase activity in rabbits

Effect of Staphylococcus Aureus infection on plasma creatine kinase activity in rabbits

Fluvastatin-induced alterations of skeletal muscle function in hypercholesterolaemic rats

Impact of different blood sampling techniques on plasma biomarkers for skeletal myopathy in conscious rats

Contact Info

Product

Resources

About