Influence of hydration states on the acute nephrotoxic effect of gentamicin in the rat

Obatomi, David K.; Plummer, David T.

doi:10.1016/0300-483x(93)90177-t

Cited by 13 publications

(5 citation statements)

References 17 publications

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“…In kidney, I/R results in accumulation of free oxygen radicals. Renal cells exhibit defensive mechanisms including various antioxidative enzymes including SOD [23]. A reduced antioxidative capacity, which is known to occur after I/R, is associated with lipid peroxidation and injury to the graft.…”

Section: Discussionmentioning

confidence: 99%

Donor Preconditioning with Taurine Protects Kidney Grafts from Injury After Experimental Transplantation

Guan

Dei-Anane

Liang

et al. 2008

Journal of Surgical Research

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Section: Discussionmentioning

confidence: 99%

Donor Preconditioning with Taurine Protects Kidney Grafts from Injury After Experimental Transplantation

Guan

Dei-Anane

Liang

et al. 2008

Journal of Surgical Research

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“…Renal cells exhibit defensive mechanisms including various anti-oxidant enzymes, i.e. SOD [10], which is one of the enzymes that eliminate free radicals in cells. Zhang [44] indicated that the effect of DS on elimination of superoxide anions is greater compared with SOD.…”

Section: Ds Protects Kidney Grafts From Irimentioning

confidence: 99%

“…Generally, renal cells exhibit defensive mechanisms including various anti‐oxidative enzymes, i.e. superoxide dismutase (SOD) [10]. Decreased SOD‐levels are present after I/R and are associated with lipid peroxidation and injury to the graft.…”

Section: Introductionmentioning

confidence: 99%

Danshen protects kidney grafts from ischemia/reperfusion injury after experimental transplantation

Guan¹,

Dei-Anane²,

Bruns³

et al. 2008

Transplant International

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Summary Danshen (DS) is used for treatment of various ischemic events in the traditional Chinese medicine. Hence, this study was designed to investigate its effect on ischemia/reperfusion injury (IRI) after experimental kidney transplantation (eKTx). Nephrectomized Sprague–Dawley rats underwent eKTx. Some animals were infused with 1.5 ml DS 10 min before surgery. Kidney grafts were transplanted after cold storage for 20 h in Histidine–Tryptophane–Ketoglutarate solution. After reperfusion blood samples were collected for blood urinary nitrogen (BUN), creatinine, lactate dehydrogenase (LDH), and alanine transaminase. Further, tissue was assessed for morphologic and pathophysiologic changes. Donor preconditioning with DS (DS‐d) significantly decreased BUN, creatinine, LDH, and aspartate aminotransferase to 65–97% of controls while preconditioning of the recipient (DS‐r) decreased values to 58–82% (P < 0.05). Tubular damage and caspase‐3 decreased significantly in both DS‐d and DS‐r (DS‐d: 96% and 67%, DS‐r: 83% and 75% of controls) while heat shock protein 72 and superoxide dismutase increased significantly (DS‐d: 143% and 173%, DS‐r: 166% and 194% of controls). Further, inducible nitric oxide synthase and tumor necrosis factor‐α decreased (DS‐d: 84% and 61%, DS‐r: 79% and 67% of controls) after DS. Preconditioning of both donors and recipients with DS significantly reduces IRI and thus improves graft function after eKTx.

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“…injection of saline (10 ml/kg) solution prior to injection of GTTR, hydrating the animals. Hydration influences the pharmacodynamics of ototoxic agents [Vogelzang, 1991;Obatomi and Plummer, 1993], and is likely responsible for the faster serum kinetics of GTTR in this study. Pretreatment with systemic injections of histamine or serotonin did not significantly affect serum concentrations of GTTR compared to the hydrated control animals in this study.…”

Section: Serum Kineticsmentioning

confidence: 99%

Infection-Mediated Vasoactive Peptides Modulate Cochlear Uptake of Fluorescent Gentamicin

2010

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Inflammatory mediators released during bacterial infection include vasoactive peptides such as histamine and serotonin, and their serum levels are frequently elevated. These peptides also modulate the vascular permeability of endothelial cells lining the blood-brain and blood-labyrinth barriers (BLB). These peptides may also modulate the permeability of the BLB to ototoxic aminoglycoside antibiotics prescribed to resolve bacterial sepsis. To test this hypothesis, we compared the effect of histamine and serotonin on the cochlear distribution of fluorescently conjugated gentamicin (GTTR) in control animals at 0.5, 1 and 3 h after injection of GTTR. The intensity of GTTR fluorescence was attenuated at 1 h in the histamine group compared to control mice, and more intense 3 h after injection (p < 0.05). In the serotonin group, the intensity of GTTR fluorescence was attenuated at 0.5 and 1 h (p < 0.05) and was increased at 3 h compared to control animals, where GTTR intensities peaked at 1 h and then plateaued or was slightly decreased at 3 h. This biphasic pattern of modulation was statistically significant in the apical turn of the cochlea. No difference in the intensity of GTTR fluorescence was observed in kidney proximal tubules. Systemic increases in serum levels of vasoactive peptides can modulate cochlear uptake of gentamicin, likely via permeability changes in the BLB. Conditions that influence serum levels of vasoactive peptides may potentiate aminoglycoside ototoxicity.

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Influence of hydration states on the acute nephrotoxic effect of gentamicin in the rat

Cited by 13 publications

References 17 publications

Donor Preconditioning with Taurine Protects Kidney Grafts from Injury After Experimental Transplantation

Donor Preconditioning with Taurine Protects Kidney Grafts from Injury After Experimental Transplantation

Danshen protects kidney grafts from ischemia/reperfusion injury after experimental transplantation

Infection-Mediated Vasoactive Peptides Modulate Cochlear Uptake of Fluorescent Gentamicin

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