The present study was aimed at elucidating the effects of feeding different forms of Humulus scandens (Hu) on performance and bacterial communities in piglets. A total of 160 piglets were divided into 4 groups of (1) CG, basal diet; (2) HS, basal diet + Hu pulp; (3) HSJ, basal diet + Hu juice; (4) HSR, basal diet + Hu residue. Results showed that HS, HSJ and HSR supplementation led to rich average daily gain (ADG) and poor feed conversion ratio (FCR) during 28-70 days of age, increased 120 d body weight (BW), average daily feed intake (ADFI) and ADG and decreased FCR during 71-120 days of age. Three experiment groups presented greater (P < 0.05) IgA, IgG, and IgM and lower (P < 0.05) glucose (GLU) and blood urea nitrogen (BUN). The content of diamine oxidase (DAO) significantly decreased (P < 0.05) in HS group. The total protein (CP) and crude fibre (CF) digestibility were improved (P < 0.05) in HS group and the Ca digestibility was increased (P < 0.05) in HS and HSJ groups. HSR supplementation improved the abundance of Firmicutes and decrease the abundance of Bacteroidetes. Hu supplementation with different form increased the proportion of Lactobacillus in cecum content. These results indicated that supplemental feeding of Hu with different form improved serum immunity, nutrient digestibility and bacterial communities in piglets, promoting growth and development, which may regard as a reference for developing novel feed resource for piglets.