Influence of<i>Lactobacillus buchneri</i>as silage additive and forage:concentrate ratio on the growth performance, fatty acid profile in<i>longissimus</i>muscle, and meat quality of beef cattle

Bragiato

Animal Feed Science and Technology

et al. 2018

The objective of this research was to investigate the effects of inoculation of corn silage and dietary supplementation of amylolytic enzymes, and their interaction, on lamb growth performance and carcass and meat traits. Whole-crop corn forage was treated either with water (untreated) or with Lactobacillus plantarum MA 18/5U at 1 × 10 5 cfu/g fresh forage combined with Bacillus subtilis AT553098 at 1 × 10 5 cfu/g fresh forage (inoculated) and ensiled for 170 d. Forty non-castrated Texel × Dorper male lambs were arranged in a randomized block design (n = 10) and then assigned to one of four diets, as follows: 1) untreated corn silage with no amylolytic enzyme supply (NI-NA); 2) untreated corn silage with amylolytic enzyme supply (NI-WA); 3) inoculated corn silage with no amylolytic enzyme supply (WI-NA); and 4) inoculated corn silage with amylolytic enzyme supply (WI-WA). α-Amylase (602 dextrinizing units/kg of dry matter (DM)) was supplied to the lambs at total mixed ration delivery. Inoculation of corn silage increased (P = 0.003) both digestible energy (DE) and metabolizable energy (ME) intake by 1.4 and 1.2 MJ/kg DM, respectively, and also resulted in higher average daily gain (ADG; P = 0.023) of lambs (233 vs. 212 g/d in lambs fed NI diet). In contrast, dietary supplementation with amylolytic enzymes had no effect on the DM intake (P = 0.90) or ADG (P = 0.15) of lambs. Moreover, inoculation, enzyme supplementation, and their interaction had no significant effects on carcass traits (P ≥ 0.06), commercial cut yield (P ≥ 0.26), chemical composition of meat (P ≥ 0.13), and meat tenderness and color (P ≥ 0.11). In terms of the fatty acid composition of longissimus muscle, inoculation of corn silage increased concentrations of saturated fatty acids (SFA; P = 0.035) from 46.2% to 47.6%, and reduced concentrations of unsaturated fatty acids (UFA; P = 0.035) from 53.8% to 52.5%, compared to the NI diet. Inoculation also reduced the UFA:SFA ratio (P = 0.031) from 1.17 to 1.11, a small but significant difference. Dietary supplementation with amylolytic enzymes did not alter (P ≥ 0.09) SFA, UFA, and polyunsaturated

Section: Discussionsupporting

confidence: 90%

Inoculation of corn silage with Lactobacillus plantarum and Bacillus subtilis associated with amylolytic enzyme supply at feeding. 2. Growth performance and carcass and meat traits of lambs

Bragiato

Animal Feed Science and Technology

et al. 2018

“…The trial was carried out over three consecutive 19‐day periods with no interval between them. A 12‐day period was allocated for the wethers to adapt to the diets based on previous studies assessing the growth performance of large and small ruminants fed inoculated silage in our laboratory (Basso et al., ; Rabelo, Basso, et al., ). Wethers were assigned to one of three diets: (i) untreated maize silage (untreated), (ii) inoculated maize silage (inoculated), and (iii) untreated maize silage, supplemented with a daily dose of L. buchneri CNCM I‐4323 injected directly into the rumen (1 × 10 7 cfu/g supplied silage; LB‐probiotic).…”

Section: Methodsmentioning

confidence: 99%

“…Man, Rogosa and Sharpe (MRS) agar was used to count LAB by pour plate, whereas potato dextrose agar (PDA) was used to count yeasts and moulds by spread plate. Both MRS and PDA plates were incubated at 28°C according to protocols used in previous studies carried out in our laboratory (Basso et al., ; Lara et al., ; Rabelo, Basso, et al., ). Lactic acid bacteria and yeasts were counted after 2 days, and moulds were counted after 5 days.…”

Section: Methodsmentioning

confidence: 99%

“…The trial was carried out over three consecutive 19-day periods with no interval between them. A 12-day period was allocated for the wethers to adapt to the diets based on previous studies assessing the growth performance of large and small ruminants fed inoculated silage in our laboratory (Basso et al, 2014;Rabelo, Basso, et al, 2016).…”

Section: Animal Studymentioning

confidence: 99%

“…Lactobacillus buchneri is a heterofermentative lactic acid bacteria (LAB) used to enhance the aerobic stability of silages by anaerobically converting lactic acid to acetic acid (Driehuis, Oude Elferink, & Spoelstra, ; Oude Elferink et al., ). Some studies have reported increased dry‐matter intake (DMI), feed efficiency and growth performance in animals fed silages inoculated with L. buchneri than in animals fed untreated silages (Basso et al., ; Nkosi et al., ; Rabelo, Basso, et al., ). In these cases, the most likely explanation of the higher animal growth performance is a change in the fermentation end products produced inside the silo.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Lactobacillus buchneri as a silage inoculant and as a probiotic on feed intake, apparent digestibility and ruminal fermentation and microbiology in wethers fed low‐dry‐matter whole‐crop maize silage

Basso

Grass and Forage Science

et al. 2017

Lactobacillus buchneri was investigated as a silage inoculant and as a probiotic on feed intake, apparent digestibility, and ruminal fermentation and microbiology in wethers fed low‐dry‐matter (DM) whole‐crop maize silage. Maize forage (279 g/kg DM) was ensiled without inoculant (untreated) and with L. buchneri CNCM I‐4323 at 1 × 105 cfu/g fresh forage (inoculated). Six cannulated wethers were arranged in a double 3 × 3 Latin square and assigned to one of three diets: (i) untreated maize silage (untreated), (ii) inoculated maize silage (inoculated), and (iii) untreated maize silage with a daily dose of L. buchneri (1 × 107 cfu/g supplied silage) injected directly into the rumen (LB‐probiotic). Wethers fed the inoculated diet had a higher (p = .050) DM intake (1.30% body weight [BW]) than wethers fed untreated and LB‐probiotic diets (1.17% and 1.18% BW respectively). The relative proportion of Ruminococcus flavefaciens (proportion of total estimated rumen bacterial 16S rDNA) in the rumen of wethers fed inoculated and LB‐probiotic diets (both 0.42%) tended (p = .098) to be lower than in the untreated diet (0.83%). Lactobacillus buchneri as a silage inoculant or as a probiotic had little effect on the variables measured in wethers.

Inoculation of corn silage with Lactobacillus plantarum and Bacillus subtilis associated with amylolytic enzyme supply at feeding. 1. Feed intake, apparent digestibility, and microbial protein synthesis in wethers

Bragiato

Animal Feed Science and Technology

et al. 2018

The current study aimed to investigate the effects of corn silage inoculation and dietary supplementation with amylolytic enzymes, and their interactions, on feed intake, apparent digestibility, and microbial protein synthesis in wethers. Whole-crop corn forage was ensiled either with water (untreated) or with Lactobacillus plantarum MA 18/5U at 1 × 10 5 cfu/g fresh forage combined with Bacillus subtilis AT553098 at 1 × 10 5 cfu/g fresh forage (inoculated). Eight ruminally cannulated Dorper × Santa Inês crossbred wethers were used in a double 4 × 4 Latin square design. Wethers were assigned to one of four diets, as follows: 1) untreated corn silage with no amylolytic enzyme supply (US-NE); 2) untreated corn silage with amylolytic enzyme supply (US-WE); 3) inoculated corn silage with no amylolytic enzyme supply (IS-NE); and 4) inoculated corn silage with amylolytic enzyme supply (IS-WE). α-Amylase (602 dextrinizing units/kg of dry matter (DM)) was supplied to the wethers at total mixed ration delivery. Dietary supplementation with amylolytic enzyme increased (P = 0.045) DM and starch (P = 0.014) intake in wethers fed the US diet (+245 and +136 g/d, respectively), but not in those fed the IS diet. Inoculation of corn silage (P = 0.026) improved organic matter (OM), crude protein (CP), neutral detergent fiber (aNDFom), and gross energy (GE) digestibility by 5.3%, 11.5%, 25.8%, and 7.1%, respectively. Likewise, enzyme supply increased (P = 0.042) the apparent digestibility of DM, OM, CP, aNDFom, and GE by 5.1%, 4.7%, 9.4%, 20.7%, and 6.2%, respectively. Enzyme supply increased the molar proportion of propionic acid (P = 0.002; 24.1 vs. 19.6 mM / 100 mM) in wethers fed the US diet, but not in those fed the IS diet. Enzyme supply decreased microbial N synthesis (P = 0.025; 6.33 vs. 8.05 g/d) when the wethers were fed the IS diet, but not the US diet. In conclusion, despite the improved OM digestibility, dietary supplementation