Influence of incubation conditions on biofilm formation by Pseudomonas fluorescens isolated from dairy products and dairy manufacturing plants

Rossi, Chiara; Chaves‐López, Clemencia; Serio, Annalisa; Goffredo, Elisa; Cenci‐Goga, Beniamino T.; Paparella, Antonello

doi:10.4081/ijfs.2016.5793

Cited by 42 publications

(39 citation statements)

References 22 publications

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“…Thus, we suggest on day three, a large number of bacteria in the plate were switched to biofilm. An optimum number of bacteria adherence occurred in microtiter plate resulting strong biofilm formation on that day (Jama et al 2017;Rossi et al 2016). In addition to day three, we observed that the biofilm formation by P. aeruginosa and S. pyogenes were strong on days four, five and six.…”

Section: Resultssupporting

confidence: 58%

Determination of optimum incubation time for formation of Pseudomonas aeruginosa and Streptococcus pyogenes biofilms in microtiter plate

et al. 2019

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Pseudomonas aeruginosa and Streptococcus pyogenes are the most common pathogens to humans and are able to form a biofilm following ineffective precautionary approach. Biofilm is defined as a surface-attached community of bacterium embedded in an extracellular matrix which leads to tremendous problems in the environment, among humans and animals. This study aims to investigate the ability of P. aeruginosa and S. pyogenes to form biofilms in 96-well plate before further study in antibiofilm will be done. Initially, the 96-well plate was added with 100 μl of overnight P. aeruginosa culture with optical density (OD) 0.1 and S. pyogenes culture with OD 0.05. The cultures were incubated for 7 days at 37°C to justify the formation of biofilm. Subsequently, stained blue biofilm was detached from the plate by using 95% ethanol. Biofilms were finally measured using a micro plate reader at 570 nm and were classified based on the adherence strength formula. P. aeruginosa and S. pyogenes biofilms strongly adhered to the plates on days three, four, five and six. Interestingly on day three, biofilms showed the highest formation. However, moderate biofilm formation onto the plates by both P. aeruginosa and S. pyogenes were observed on day two, but non-adherence was observed on days one and seven. Day three is the optimum cultivation period for P. aeruginosa and S. pyogenes to switch into a strong biofilm in microtiter plate and could be beneficial for antibiofilm experiments.

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Section: Resultssupporting

confidence: 58%

Determination of optimum incubation time for formation of Pseudomonas aeruginosa and Streptococcus pyogenes biofilms in microtiter plate

et al. 2019

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“…Indeed, P. fluorescens has the ability to adapt even to hostile environments, e.g., cold rooms, where the conditions for its growth are not optimal [3,4]. The ability of these microorganisms to adapt to different environments is likely accounted for by the formation of biofilms, making the bacterial population very resistant [28,29].…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas azotoformans Belonging to Pseudomonas fluorescens Group as Causative Agent of Blue Coloration in Carcasses of Slaughterhouse Rabbits

Circella

Schiavone

Barrasso

et al. 2020

Animals

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The study describes the finding of an abnormal blue-tinged color found on rabbit carcasses in the refrigeration cell of two butcher shops in Apulia Region. The carcasses were from an industrial rabbitry for production of meat with a regularly authorized slaughterhouse. Pseudomonas azotoformans, a microorganism included in Pseudomonas fluorescens group, was isolated from samples collected by the altered carcasses, showing the growth of uniform bacterial colonies with fluorescent pigmentation. The bacterium was also isolated from an additional water sample and from the labelling gun collected in the slaughterhouse, whilst the knives used for slaughtering resulted negative. Chromatic alteration was experimentally reproduced on new carcasses using a 108 cfu/mL bacterial suspension prepared with the isolated strain. Due to their resistance characteristics, members of P. fluorescens group are very difficult to eradicate once introduced into the production environment. Therefore, their presence, even if not considered a public health problem, should be monitored by food industry operators in self-control plans.

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“…This proves that after the extended incubation, the biofilm formed on the peg lids have more time to recruit more bacteria in the biofilm establishing denser and larger colony on EMB agar. [32] Within 24 hrs, concentrations 1:1 and 2:3 both exhibited pinpoint colonies in quadrants I, II and III while showed small colonies in quadrant IV. Concentration 1.5:3.5 produced small colonies in quadrants I, II and III whereas medium colonies in quadrant IV.…”

Section: Resultsmentioning

confidence: 95%

“…This proves that as the incubation prolongs, it gives more time for the bacteria to aggregate and combine with one another constructing one large colony. [32] Colonies also gave slightly pink appearance owing to its capability to ferment glucose due to the presence of E. coli and these appeared to be mucoid due to the extracellular polysaccharide matrix produced during the biofilm formation.…”

Section: Resultsmentioning

confidence: 99%

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Role of Pseudomonas aeruginosa Biofilm in the Development of Antibiotic Resistance in Escherichia coli

Raquid¹,

Prieto²,

Arciaga³

et al. 2018

AJBLS

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The study aimed to assess the role of Pseudomonas aeruginosa (P. aeruginosa) biofilm in the development of antimicrobial resistance of Escherichia coli (E. coli). P. aeruginosa (ATCC 27853) and E. coli (ATCC 25922) were cultured on cetrimide agar and eosin methylene blue (EMB) agar. The two microorganisms were mixed to prepare three bacterial suspensions using tryptic soy broth (TSB) in increasing concentrations, such as: 1:1, 2:3 and 1.5:3.5. Bacterial suspensions were transferred into MBEC™ Assay kits which was subjected to agitation and incubated at 37°C for 24 hrs and 48 hrs. Formed biofilms on peg lids were obtained for Gram staining and cultured using EMB agar to evaluate the biofilm matrix and presence of bacteria. Polymerase chain reaction (PCR) was conducted to determine significant changes in E. coli genes that matched the Tn1696 aacC1, Tn10 tetRA and Tn903 aph resistance genes of E. coli which codes for gentamicin, tetracycline and kanamycin, respectively. Results showed strong bands to Tn1696 aacC1 gene for gentamicin resistance at the highest concentration. No bands were visualized for tetracycline and kanamycin resistance. The results therefore establish that coexistence of both organisms in a biofilm leads to the development of the antimicrobial resistance of E. coli to gentamicin.

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Influence of incubation conditions on biofilm formation by Pseudomonas fluorescens isolated from dairy products and dairy manufacturing plants

Cited by 42 publications

References 22 publications

Determination of optimum incubation time for formation of Pseudomonas aeruginosa and Streptococcus pyogenes biofilms in microtiter plate

Determination of optimum incubation time for formation of Pseudomonas aeruginosa and Streptococcus pyogenes biofilms in microtiter plate

Pseudomonas azotoformans Belonging to Pseudomonas fluorescens Group as Causative Agent of Blue Coloration in Carcasses of Slaughterhouse Rabbits

Role of Pseudomonas aeruginosa Biofilm in the Development of Antibiotic Resistance in Escherichia coli

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