Influence of Indole Butyric Acid on the Micropropagation ofLamium AlbumL.

Dimitrova, Mariya; Yordanova, Zhenya; Dragolova, D.; Kapchina-Toteva, Veneta

doi:10.1080/13102818.2010.10817808

Cited by 3 publications

(4 citation statements)

References 11 publications

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“…In our study, high BAP concentrations showed efficient micropropagation and multiplication with the combination of GA3. Dimitrova et al (2010) investigated the effect of auxin on micropropagation of Lamium album and obtained the highest shoot length on MS medium supplemented with 0.5 mg/L IBA. Additionally, Dimitrova et al (2011) and mean root number were determined as (5.4±1.2) on MS medium including 0.7 mg/L IBA.…”

Section: Resultsmentioning

confidence: 99%

“…(Sevindik et al 2019;Yavuz 2016;Shtereva et al 2015;Faria et al 1998;Sánchez-Gras and Segura 1997;Garcia-Granados et al1994;Sánchez-Gras and Segura 1988), but only a few report were published about in vitro propagation of Lamium sp. Most of the studies were focus on micropropagation of Lamium album (Dimitrova et al 2010(Dimitrova et al , 2011Stefanova et al 2011). In this study we investigate the effect of different combinations of BAP and GA3 on node explants of L. garganicum.…”

Section: Resultsmentioning

confidence: 99%

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Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey

Sevindik¹

2020

CJAFS

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Lamiaceae family includes annual and perennial 250 genus which are commonly used for their medicinal aromatic properties due to its high biologically active compounds. Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum (Telbalıcak) is a member of Lamium genus spread over in Turkey. Lamium species are used for cure since ancient times. Tissue culture provides important advantages for the conservation and sustainability of genetic resources such as clonal and mass production in a limited area. Micropropogation is one of the most significant alternatives for rapid clonal propagation in a short time. In this study, nodes include one or two leaves were placed on MS medium including different levels of BA and GA3 (0, 0.5, 1, 1.5, 2, 2.5 mg/L) for propagation. The explants regenerated to the shoots with the response of 82.5% and 80% in MS medium including 2 mg/L BA +2 mg/L GA3, 2 mg/L BA +1.5 mg/L GA3 respectively. Rooting experiment was carried on MS medium supplied with 0, 0.5, 1, 1.5, 2 mg/L Indole-3-Butyric Acid (IBA) for root induction. Maximum root formation was observed as 45% on MS medium including 0.5 mg/L IBA. Plantlets were acclimatized and 80% of the plants are survived after acclimatization.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey

Sevindik¹

2020

CJAFS

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“…Cytokinins regulate leaf development by affecting cell division, photosynthesis, chloroplast development, senescence and assimilate partitioning (Binns 1994) and are very important for development of the plant photosynthetic apparatus through their direct role in chloroplast differentiation (Chernyad'ev 2000, Kulaeva 2002, Arigita et al 2005. Auxins are known to promote cell elongation, cell division and formation of adventitious roots (Dimitrova et al 2010). The influence of cytokinins on the leaf structure of in vitro grown plants often has been subject of scientific research (Wetzstein and Sommer 1982, Olmos and Hellín 1998, Paek and Hahn 2000, Sudriá et al 2001, Toma et al 2004, Deccetti et al 2008, De Oliveira et al 2008, Magyar-Tábori et al 2010, while the effect of auxins has been less investigated (Sudriá et al 2001, Toma et al 2004.…”

Section: Introductionmentioning

confidence: 99%

Effect of N6-benzyladenine and indole-3-butyric acid on photosynthetic apparatus of Orthosiphon stamineus plants grown in vitro

Stoyanova-Koleva¹,

Stefanova²,

Zhiponova³

et al. 2012

Biologia plant.

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The leaf structure and chloroplast ultrastructure of kidney tea (Orthosiphon stamineus Benth.) was studied in in vitro culture on standard MS medium supplemented with or without plant growth regulators (PGRs). The cytokinin N 6 -benzyladenine (BA) negatively affected the structure of the palisade parenchyma and chloroplast ultrastructure and increased the stomatal frequency of the adaxial epidermis. The auxin indole-3-butyric acid (IBA) did not modify the morphology of regenerated leaf tissues as well as the chloroplast ultrastructure. The effect of both PGRs applied in combination was manifested in well-differentiated mesophyll parenchyma, typical chloroplast ultrastructure and increased stomatal frequency on both leaf surfaces. This protocol can be suggested for further ex vitro propagation.

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“…In vitro cultures were induced from sterilized mono-nodal stem segments of the mature wild plants. The plants were sterilized and propagated under controlled environmental conditions in vitro (Dimitrova et al, 2010), and after four weeks of cultivation they were collected and air-dried. For the in vivo variants (leaves and flower), the mature plant material was harvested from the natural habitats and dried in the shade for grinding.…”

Section: Plant Materialsmentioning

confidence: 99%

Antimicrobial activity of extracts from in vivo and in vitro propagated Lamium album l. plants

Chipeva¹,

Petrova²,

Geneva³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

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The antimicrobial activity of 18 different extracts from in vivo and in vitro grown L. album L. plants was evaluated against clinical bacteria and yeasts using the well diffusion method. All the used extracts demonstrated antibacterial activity, whereas only the water extracts from leaves (in vivo) possessed antifungal activity against Candida albicans NBIMCC 72 and Candida glabrata NBIMCC 8673 (14 and 20 mm diameter of inhibition zones and MIC 10 mg/ml, respectively). The methanol and ethanol extracts obtained from the in vitro propagated plants had a broader spectrum of antibacterial activity than those from in vivo plants, while the opposite tendency was observed for the chloroform extracts. All tested flower extracts possessed antimicrobial activity. The chloroform extract from in vivo flowers demonstrated the highest activity against E. faecalis NBIMCC 3915, S. aureus NBIMCC 3703, P. hauseri NBIMCC 1339 and P. aeruginosa NBIMCC 3700 (22 mm, 13 mm, 11 mm, 23 mm zone diameter of inhibition and MIC 0.313 mg/ml, respectively). The water extracts from leaves (both in vivo and in vitro) possessed higher antibacterial activity than extract from flowers. The obtained results showed that both in vivo and in vitro propagated L. album L. could be used as a source of antibacterial substances.

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Influence of Indole Butyric Acid on the Micropropagation ofLamium AlbumL.

Cited by 3 publications

References 11 publications

Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey

Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey

Effect of N<sup>6</sup>-benzyladenine and indole-3-butyric acid on photosynthetic apparatus of Orthosiphon stamineus plants grown in vitro

Antimicrobial activity of extracts from <i>in vivo</i> and <i>in vitro</i> propagated <i>Lamium album</i> l. plants

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