Influence of infliximab pretreatment on ischemia/reperfusion injury in rat intestine

Abstract

Abstract. The Pringle maneuver is used in hepatic surgery to prevent blood loss but is associated with ischemia-reperfusion injury. To investigate the effect of infliximab on inflammation and apoptosis in rat intestinal mucosa during ischemia-reperfusion (IR) injury. A total of 30 male Wistar albino rats were equally divided into three groups to be subjected to (i) sham operation (sham), (ii) IR injury via Pringle maneuver (pringle) or (iii) infliximab (IFX) group (IFX was given at a dose 3 mg/kg for 3 days before IR injury). Following reperfusion period of 60 min., intestinal tissue and blood samples were taken and processed by standard histological methods. The Pringle maneuver and following reperfusion caused significant histopathological changes, increased serum transaminases' activity and the levels of oxidative stress markers and decreased glutathione peroxidase activity. IFX pretreatment partially prevented these changes. Infliximab pretreatment may protect intestinal mucosa against ischemia-reperfusion injury. Further studies are needed to investigate mechanism and evaluate safety and optimal dosing of IFX in humans exposed to the possible tissue damage by ischemia-reperfusion.

Tnf-α inhibition by infliximab as a new target for the prevention of glycerol-contrast-induced nephropathy

“…In a previously conducted study, infliximab was shown to decrease the raised antioxidant levels in hepatic injury induced by paracetamol (Ferah et al, 2013). In another study in rats, infliximab was shown to decrease the elevated oxidative stress of ischemic intestinal injury back to healthy levels (Akdogan et al, 2014). All these results support our findings and clearly show the protective role of infliximab against oxidative stress.…”

Preconditioning with the BKCa channel activator NS-1619 prevents ischemia-reperfusion-induced inflammation and mucosal barrier dysfunction: roles for ROS and heme oxygenase-1

“…Although these data suggest that TNF-␣ might serve as a useful proinflammatory marker, our data could be interpreted to suggest that the cytokine may not be a direct cause of inflammation in postischemic intestine. However, this proposal is not supported by the observations that TNF immunoneutralization or inhibition prevents postischemic leukocyte adhesion, phagocyte infiltration, and tissue injury in the small intestine (1,20,50,74), suggesting a cause-and-effect relationship. Similarly, mice deficient in TNF-␣ receptor 1 also demonstrated marked reductions in P-selectin, E-selectin, VCAM and ICAM-1 expression, intestinal neutrophil infiltration, and apoptosis after splanchnic I/R compared with WT counterparts (11).…”

Study of Morphological Changes in Rat Liver Caused by Occlusion of Inferior Vena Cava

“…Microtome sections of the small intestine 4 mm thick were cut and then stained with hematoxylin and eosin. 20 Two independent pathologists have performed the histological evaluation. Such an evaluation procedure was carried out immediately after confirmation of death in order to avoid degenerative changes in organs.…”