Influence of irrigant needle depth in removing bioluminescent bacteria inoculated into instrumented root canals using real‐time imaging <i>in vitro</i>

Sedgley, Christine M.; Nagel, Aaron; Hall, Dan; Applegate, Bruce

doi:10.1111/j.1365-2591.2004.00906.x

Cited by 161 publications

(161 citation statements)

References 30 publications

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“…They determined the correlation between bioluminescent signal and extracted CFUs. This group went on to use this model to study the effect of varying the needle depth during endodontic irrigation [52].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection

et al. 2006

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Background and Objective: To compare the effectiveness of antimicrobial photodynamic therapy (PDT), standard endodontic treatment and the combined treatment to eliminate bacterial biofilms present in infected root canals. Study Design/Materials and Methods: Ten singlerooted freshly extracted human teeth were inoculated with stable bioluminescent Gram-negative bacteria, Proteus mirabilis and Pseudomonas aeruginosa to form 3-day biofilms in prepared root canals. Bioluminescence imaging was used to serially quantify bacterial burdens. PDT employed a conjugate between polyethylenimine and chlorin(e6) as the photosensitizer (PS) and 660-nm diode laser light delivered into the root canal via a 200-m fiber, and this was compared and combined with standard endodontic treatment using mechanical debridement and antiseptic irrigation. Results: Endodontic therapy alone reduced bacterial bioluminescence by 90% while PDT alone reduced bioluminescence by 95%. The combination reduced bioluminescence by > 98%, and importantly the bacterial regrowth observed 24 hours after treatment was much less for the combination (P < 0.0005) than for either single treatment. Conclusions: Bioluminescence imaging is an efficient way to monitor endodontic therapy. Antimicrobial PDT may have a role to play in optimized endodontic therapy. Lasers Surg.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection

et al. 2006

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“…It has been shown that the penetration depth of the irrigation needle affects the extrusion of irrigants, with better cleaning when the irrigation needle is placed. 23,24 The 27-gauge sidevented needle tip was inserted passively and never allowed to bind as the irrigant was being deposited into the canal. However, the SAF's hollow design allows for continuous irrigation throughout the procedure.…”

Section: Discussionmentioning

confidence: 99%

Apical Extrusion of Debris and Irrigant using Novel Preparation Systems

Uzunoğlu¹,

Görduysus²

2014

The Journal of Contemporary Dental Practice

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Aim:The aim of this in vitro study was to assess the amount of apically extruded debris and irrigant using Reciproc vs self-adjusting file (SAF) and to investigate the effect, if any, of gravitational force via mandibular and maxillary positioning of the teeth. Materials and methods:Forty human mandibular premolars were randomly assigned to four groups of 10 teeth each. The root canals were instrumented according to the manufacturers' instructions using the Reciproc and SAF. Sodium hypochlorite was used as the irrigant. The apically extruded debris was collected in pre-weighted glass vials using the Myers and Montgomery method, and teeth were placed in vials both in downward and upward positions. After drying, the mean weight of debris was assessed with a microbalance and statistically analyzed using two-way ANOVA with Bonferroni correction.Results: All instrumentation techniques and positions caused measurable apical extrusion of debris. A significant difference was observed according to position and instrument used (p < 0.05). The Reciproc extruded significantly more debris than SAF and vial downward position extruded significantly more debris than upward position (p < 0.05). Conclusion:Under the conditions of this study, all systems caused apical debris extrusion, especially in the downward position.Clinical significance: According to results of this study, the SAF was associated with less debris extrusion compared to the Reciproc in both mandibular and maxillary positions.

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“…Closed-ended irrigant tips are side-vented and thus create more pressure on the walls of the root canal and improve the hydrodynamic activation of an irrigant and reduce the chance of apical extrusion. This allows the irrigant to reflux and causes more debris to be displaced coronally, while avoiding the inadvertent expression of the irrigant into periapical tissues [6].…”

Section: Needle-tip Designmentioning

confidence: 99%

Pressure Alteration Techniques in Endodontics- A Review of Literature

Pasricha¹,

Makkar²,

Gupta

2015

JCDR

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Influence of irrigant needle depth in removing bioluminescent bacteria inoculated into instrumented root canals using real‐time imaging in vitro

Abstract: The mechanical efficacy of 6 mL of irrigant in reducing intracanal bacteria was significantly greater when delivered 1 mm compared with 5 mm from WL.

Cited by 161 publications

References 30 publications

Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection

Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection

Apical Extrusion of Debris and Irrigant using Novel Preparation Systems

Pressure Alteration Techniques in Endodontics- A Review of Literature

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