Influence of Islet Transportation on Pancreatic Islet Allotransplantation in Type 1 Diabetic Patients Within the Swiss-French Gragil Network

Kessler, L.; Bucher, Pascal Alain Robert; Guittard, Laure; Benhamou, Pierre Yves; Berney, Thierry; Penfornis, A.; Badet, Lionel; Thivolet, Charles; Bayle, François; Oberholzer, José; Renoult, E.; Brun, J; Rifle, G; Atlan, Catherine; Colin, Cyrille; Morel, Philippe

doi:10.1097/01.tp.0000122223.79315.5d

Cited by 36 publications

(24 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 'islet consortium' concept offers obvious advantages over establishing a new ICPC at each site. Sharing of donor pancreata and isolated islets between centers may also be of assistance in maximizing utilization of donor pancreata (9,10). In this study, we have evaluated the shipment protocol utilized at our ICPC consisting on the use of GPBs, which…”

Section: Discussionmentioning

confidence: 99%

“…Shipping protocols of islet preparations are less detailed. In the GRAGIL (Groupe Rhin-Rhone-AlpesGeneve pour la Transplantation d'Ilots de Langerhans) experience, islets loaded in a syringe or infusion bag are transported by ambulance to the remote transplant centers where they are transplanted within hours (9,10). Similarly, the University of California Islet Transplant Consortium reported shipping islets in the infusion bag to the remote centers within hours from isolation or after culture in RPMI medium at 37…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Shipment of Human Islets for Transplantation

Ichii¹,

Sakuma²,

Pileggi³

et al. 2007

American Journal of Transplantation

105

View full text Add to dashboard Cite

The use of regional human islet cell processing centers (ICPC) supporting distant clinical islet transplantation programs (CITP) has proven successful in recent clinical trials. Standardization of islet shipping protocols is needed to preserve cell product identity, quantity, quality and sterility, and to meet criteria for transplantation. We evaluated the use of gas-permeable bags for human islet preparation shipment from a single ICPC to two remote CITPs. Product release tests (counts, purity, viability, sterility and potency) were performed at both centers using identical protocols to determine adequacy for transplantation. Thirty-five islet preparations were shipped either immediately after isolation (n = 20) or following culture (n = 15). Islet recovery rate after shipment was higher in cultured preparations, when compared to those not cultured (91.2 ± 4.9% vs. 72.9 ± 4.7%, respectively; p < 0.05), though the overall recovery rate based on isolation and pretransplant counts was comparable (72.9 ± 4.7% vs. 70.4 ± 3.5%, respectively; p = N.S.). All preparations met product release criteria for transplantation. Additional experiments showed that gas-permeable bags led to improved recovery and potency, when compared to 50-mL conical tubes or to non-gas-permeable bags for shipment. Collectively, our data demonstrate that the use of gas-permeable bags is efficient for clinicalgrade and should be preferred also for the shipment of research-grade islet preparations.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Shipment of Human Islets for Transplantation

Ichii¹,

Sakuma²,

Pileggi³

et al. 2007

American Journal of Transplantation

105

View full text Add to dashboard Cite

show abstract

“…Following the Edmonton protocol, the transplantation of islets can successfully induce a long-term restoration of the insulin production, the normalization of the hemoglobin A1C, and the lowering of hypoglycemia excursions (Kessler et al, 2004;Ryan et al, 2005). Nowadays, islets grafting results in 80% insulin independence 1 year after transplantation.…”

Section: Introductionmentioning

confidence: 99%

Adenoviral infection or deferoxamine? Two approaches to overexpress VEGF in β-cell lines

Langlois¹,

Bietiger²,

Sencier³

et al. 2009

Journal of Drug Targeting

Self Cite

View full text Add to dashboard Cite

Rapid and adequate revascularization of transplanted islets is important for their survival and function during transplantation. Vascular endothelial growth factor (VEGF) could play a critical role with respect to islet revascularization. The aim of this study was to compare two strategies that are used to overexpress VEGF in beta-cells: (1) gene therapy through adenoviral infection and (2) a pharmacological approach using deferoxamine (DFO). beta-Cell lines from rat insulinoma (RINm5F) were either infected using an adenovirus encoding the gene of human VEGF 165 or incubated with DFO. One day after treatment, the viability of RINm5F cells was preserved with 10 micromol/L of DFO (103.95 +/- 5.66% toward control; n = 4). In addition, adenoviral infection maintained the viability of cells for all the concentrations used. In both treatments, overexpression of VEGF was in a comparable level. Finally, the ratio of Bax/Bcl-2 indicated that the apoptosis increased in infected beta-cells whereas treatment with DFO seems to be antiapoptotic. Our results suggest that the use of DFO could be a realistic approach to improve the vascularization of islets during transplantation.

show abstract

“…Furthermore, high costs are involved in establishing and maintaining a GMP-certified isolation facility. Therefore, multicenter IT networks have been established around a single islet isolation facility [2,3]. An appropriate method of islet transportation must be established to transport isolated islets from the islet isolation facility to the transplant hospitals.…”

Section: Introductionmentioning

confidence: 99%