Influence of jasmonic acid on the growth and antimicrobial and antioxidant activities of Lavandula angustifolia Mill. propagated in vitro

Abstract

The aim of the study was to determine the influence of jasmonic acid added to culture medium on the growth of plants and antioxidant properties of dry plant material, as well as on the antimicrobial properties of essential oils produced by the narrow-leaved lavender. For plant propagation, MS media supplemented with JA at concentrations of 0.2-1.5 mg dm−3 were used. The use of the lower JA concentrations did not influenced the growth parameters measured, whereas at the higher concentrations (1 and 1.5 mg dm−3) JA caused growth inhibition and a decrease in plant weight. With increasing JA concentration, the number of secretory trichomes decreased. Addition of 0.5 mg dm−3 JA caused an increase in secretory trichome diameter on both the adaxial and abaxial surface of leaves (83.3 and 73.2 μm, respectively). The antioxidant activity of the lavender plants propagated on media with the addition of JA (regardless of the concentration used) was higher than that of the control plants. The plants from JA-supplemented media were used to isolate essential oils, the antimicrobial activity of which was tested using the disc diffusion method at the concentrations of 10 and 50%. All the oils tested exhibited activity towards Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans. The essential oils isolated from the plants propagated on the medium with 1 mg dm−3 JA were characterized by the highest antimicrobial activity against the majority of the tested microorganisms.

Effect of Jasmonic Acid and Salicylic Acid on Growth and Biochemical Composition of In-Vitro-Propagated Lavandula angustifolia Mill

“…It is well-documented in the literature that the composition of the culture medium highly influences the micropropagation of lavender and determines the development, morphological parameters, and phytochemical composition of the plant tissue [3,44,45]. This study evaluated the effect of different concentrations of JA and SA, alone or in combination with AC, on the proliferation of L. angustifolia explants, their morphological parameters and phytochemical composition in terms of phenolic, chlorophyll, and carotenoid content.…”

“…Previous research indicates that JA changes L. angustifolia growth and essential oil composition [44,45], but nothing is known about its influence on the polyphenol and carotenoid contents of the plant. Similarly, there is little information on how SA alters L. angustifolia development and synthesis of bioactive compounds [44,45].…”

“…Previous research indicates that JA changes L. angustifolia growth and essential oil composition [44,45], but nothing is known about its influence on the polyphenol and carotenoid contents of the plant. Similarly, there is little information on how SA alters L. angustifolia development and synthesis of bioactive compounds [44,45]. The aim of this research was to assess the effect of JA and SA on in vitro shoot proliferation of L. angustifolia as well as on the production of bioactive compounds (polyphenols, chlorophylls, and carotenoids).…”

Antibacterial Potential of Clonally Propagated Prunella vulgaris L. under in vitro Conditions

“…Among these methods, micropropagation could be produced pharmacologically active molecules at the desired amount and constant quality at any time in laboratory conditions [14,15]. Several studies on the antimicrobial potential of medicinal plants produced by plant tissue culture attract the attention [16][17][18].…”

Phytochemical screening and chemical compound of green roselle(Hibiscus sabdariffa L.) and potential antibacterial activities

“…Physiological responses often include activation of the antioxidant system (superoxide anion radical, NADPH-oxidase, peroxidase), accumulation of soluble sugars andamino acids (isoleucine and methionine), and regulation of stomatal opening and closing. Jasmonic acid also has antimicrobial and antioxidant activities [32].…”