Annual bluegrass (Poa annua L; ABG) is susceptible to damage from ice cover. Effects of chemical treatments on ice survival under low temperature growth chamber conditions were studied. Field putting green plots of ABG were treated in the fall of 2014 and 2015 with Civitas, mefluidide, propiconazole or trinexapac‐ethyl (TE) at label rates. After acclimation, turfgrass cores from each plot were transferred to a low temperature growth chamber, received ice or no ice treatment and were sampled at 0, 20, 40 and 60 days. Ice was applied by misting plants at low temperature. In 2014, Civitas‐, mefluidide‐ and propiconazole‐treated plugs had the greatest amount of regrowth after ice and no ice treatments on most days; comparable results were found in 2015 but fewer statistical differences were detected. TE‐treated plants were no different from the untreated controls on most days in either year. Plants that were treated with mefluidide, propiconazole and Civitas had more polyunsaturated fatty acids (FA) in crown tissue, with linoleic acid approximately 50% greater compared to TE and untreated samples. Civitas, mefluidide or propiconazole treatments improved ABG survival of simulated ice conditions, which could be associated with the changes to saturated and unsaturated FA ratios that were observed in this study.