Influence of Low Molecular Weight Salts on the Viscosity of Aqueous-Buffer Bovine Serum Albumin Solutions

Zdovc, Blaž; Jaklin, Matej; Hribar-Lee, Barbara; Lukšič, Miha

doi:10.3390/molecules27030999

Cited by 8 publications

(2 citation statements)

References 38 publications

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“…To answer the above two questions, we used a standard Nd:YAG laser with a pulse duration of approximately 10 ns. Bovine Serum Albumin (BSA) was used as a model protein, as it is one of the most studied proteins, quite frequently used in biological studies [ 23 ]. Thus, in this work, for the first time, using the example of BSA protein, it was discovered how protein molecules affect the characteristics of optical breakdown in an aqueous medium, how the optical properties of protein molecules change after optical breakdown, and what changes occur in the structure of molecules.…”

Section: Introductionmentioning

confidence: 99%

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

et al. 2022

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The influence of laser radiation of a typical surgical laser on the physicochemical properties of the Bovine Serum Albumin (BSA) protein was studied. It was established that the physicochemical characteristics of optical breakdown weakly depend on the concentration of protein molecules. At the same time, the patterns observed for an aqueous solution of BSA irradiated with a laser for different time periods were extremely similar to the classical ones. It was established that after exposure to laser radiation, the optical density of protein solutions increases. At the same time, the intensity of BSA fluorescence due to aromatic amino acid residues decreases insignificantly after exposure to laser radiation. In this case, the position of the excitation and emission maximum does not change, and the shape of the fluorescence spot on 3D maps also does not change significantly. On the Raman spectrum after exposure to laser radiation, a significant decrease in 1570 cm−1 was observed, which indicates the degradation of α-helices and, as a result, partial denaturation of BSA molecules. Partial denaturation did not significantly change the total area of protein molecules, since the refractive index of solutions did not change significantly. However, in BSA solutions, after exposure to laser radiation, the viscosity increased, and the pseudoplasticity of aqueous solutions decreased. In this case, there was no massive damage to the polypeptide chain; on the contrary, when exposed to optical breakdown, intense aggregation was observed, while aggregates with a size of 400 nm or more appeared in the solution. Thus, under the action of optical breakdown induced by laser radiation in a BSA solution, the processes of partial denaturation and aggregation prevail, aromatic amino acid residues are damaged to a lesser extent, and fragmentation of protein molecules is not observed.

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Section: Introductionmentioning

confidence: 99%

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

et al. 2022

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“…This involves taking into account both the properties of the involved fluids and the geometry of our system. The culture media solution has density ρ = 1008 kg/m 3 and viscosity η = 0.733 × 10 −3 Pa•s [41], and the aqueous 0.1 M acetate buffer has ρ = 1007 kg/m 3 and η = 1 × 10 −3 Pa•s [42]. The culture medium was modelled as solvent solution entering Inlet 1, the acetate buffer was modelled as dilute species entering Inlet 2 with a diffusion coefficient in the culture medium of 1 × 10 −9 m 2 /s.…”

Section: Efficient Medium Acidification In the Adapted Commercial Pla...mentioning

confidence: 99%

Anodic Stripping Voltammetric Determination of Copper Ions in Cell Culture Media: From Transwell® to Organ-on-Chip Systems

Prontera,

Sciurti,

De Pascali

et al. 2023

Chemosensors

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The integration of sensing devices into cell culture systems is a topic of great interest in the study of pathologies and complex biological mechanisms in real-time. In particular, the fit-for-purpose microfluidic devices called organ-on-chip (OoC), which host living engineered organs that mimic in vivo conditions, benefit greatly from the integration of sensors, enabling the monitoring of specific chemical-physical parameters that can be correlated with biological processes. In this context, copper is an essential trace element whose total concentration may be associated with specific pathologies, and it is therefore important to develop reliable analytical techniques in cell systems. Copper can be determined by using the anodic stripping voltammetry (ASV) technique, but its applicability in cell culture media presents several challenges. Therefore, in this work, the performance of ASV in cell culture media was evaluated, and an acidification protocol was tested to improve the voltammetric signal intensity. A Transwell® culture model with Caco-2 cells was used to test the applicability of the developed acidification protocol by performing an off-line measurement. Finally, a microfluidic device was designed in order to perform the acidification of the cell culture medium in an automated manner and then integrated with a silicon microelectrode to perform in situ measurements. The resulting sensor-integrated microfluidic chip could be used to monitor the concentration of copper or other ions concentration in an organ-on-chip model; these functionalities represent a great opportunity for the non-destructive strategic experiments required on biological systems under conditions close to those in vivo.

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Biocompatible Liquid Embolic for the Treatment of Microvascular Hemorrhage

Keum,

Kim,

Zhang

et al. 2024

Advanced Science

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Persistent or recurrent bleeding from microvessels inaccessible for direct endovascular intervention is a major problem in medicine today. Here, an innovative catheter‐directed liquid embolic (P‐LE) is bioengineered for rapid microvessel embolization to treat small vessel hemorrhage. Tested in rodent, porcine, and canine animal models under normal and coagulopathic conditions, P‐LE outperformed clinically used embolic materials in both survival and non‐survival experiments, effectively occluding vessels as small as 40 microns with no signs of recanalization. P‐LE occlusion is independent of the coagulation cascade, and its resistance to displacement is ≈ 8 times greater than systolic blood pressure. P‐LE is also found to be biocompatible and x‐ray visible and does not require polymerization or a chemical reaction to embolize. To simulate the clinical scenario, acute microvascular hemorrhage is created in the pig kidney, liver, or stomach; these are successfully treated with P‐LE achieving immediate hemostasis. Furthermore, P‐LE is found to be bactericidal to highly resistant patient‐derived bacteria, suggesting that P‐LE may also protect against infectious complications that may occur following embolization procedures. P‐LE is safe, easy to use, and effective in treating ‐microvessel hemorrhage.

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Influence of Low Molecular Weight Salts on the Viscosity of Aqueous-Buffer Bovine Serum Albumin Solutions

Cited by 8 publications

References 38 publications

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

Anodic Stripping Voltammetric Determination of Copper Ions in Cell Culture Media: From Transwell® to Organ-on-Chip Systems

Biocompatible Liquid Embolic for the Treatment of Microvascular Hemorrhage

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