Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cells

Kim, Hyeon Joo; Kim, Ung-Jin; Vunjak-Novakovic, Gordana; Min, Byoung‐Hyun; Kaplan, David L.

doi:10.1016/j.biomaterials.2004.11.013

Cited by 293 publications

(248 citation statements)

References 42 publications

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“…To develop bone graft substitutes the simplest methodology is to statically culture cells on highly porous scaffolds in the presence of differentiation medium (Farrell, E et al, 2006;Farrell et al, 2007;Keogh et al, 2010;Kim et al, 2005;St-Pierre et al, 2005;Tierney et al, 2009). However, static culturing regimes neglect the important role of biomechanical stimuli in the normal formation, development and homeostasis of bone tissue: the effects of which are clearly observed when normal, physiologically relevant levels of mechanical stimulation are removed (Morey and Baylink, 1978;Zerwekh et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…To date, the authors are unaware of any studies directed at evaluating the detachment of cells cultured on highly porous scaffolds in response to fluid-flow. Highly porous scaffolds contain exceedingly irregular geometries as a consequence of the freeze drying (Mandal and Kundu, 2008;O'Brien et al, 2004) or salt leaching (Kim et al, 2005) fabrication processes, resulting in non-uniform flow profiles that make shear stress characterisation very challenging. Furthermore, technical limitations in visualising cells internally within the construct, means correlating cell detachment to the magnitude of shear stress at the point of detachment is exceptionally difficult.…”

Section: Introductionmentioning

confidence: 99%

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Influence of flow rate and scaffold pore size on cell behavior during mechanical stimulation in a flow perfusion bioreactor

McCoy

Jungreuthmayer

O’Brien

2012

Biotech & Bioengineering

104

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FJ. Influence of flow rate and scaffold pore size on cell behavior during mechanical stimulation in a flow perfusion bioreactor. Biotechnology and Bioengineering. 2012;109(6):1583-1594.-Use LicenceAttribution-Non-Commercial-ShareAlike 1.0 You are free:• to copy, distribute, display, and perform the work.• to make derivative works. Under the following conditions:• Attribution -You must give the original author credit.• Non-Commercial -You may not use this work for commercial purposes.• AbstractMechanically stimulating cell-seeded scaffolds by flow-perfusion is one approach utilised for developing clinically applicable bone graft substitutes. A key challenge is determining the magnitude of stimuli to apply that enhances cell differentiation but minimises cell detachment from the scaffold. In this study we employed a combined computational modelling and experimental approach to examine how the scaffold mean pore size influences cell attachment morphology and subsequently impacts upon cell deformation and detachment when subjected to fluid-flow. Cell detachment from osteoblast-seeded collagen-GAG scaffolds was evaluated experimentally across a range of scaffold pore sizes subjected to different flow-rates and exposure times in a perfusion bioreactor. Cell detachment was found to be proportional to flow-rate and inversely proportional to pore size. Using this data, a theoretical model was derived that accurately predicted cell detachment as a function of mean shear stress, mean pore size and time. Computational modelling of cell deformation in response to fluid flow showed the percentage of cells exceeding a critical threshold of deformation correlated with cell detachment experimentally and the majority of these cells were of a bridging morphology (cells stretched across pores). These findings will help researchers optimise the mean pore size of scaffolds and perfusion bioreactor operating conditions to manage cell detachment when mechanically simulating cells via flow perfusion.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of flow rate and scaffold pore size on cell behavior during mechanical stimulation in a flow perfusion bioreactor

McCoy

Jungreuthmayer

O’Brien

2012

Biotech & Bioengineering

104

View full text Add to dashboard Cite

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“…cellular proliferation [1,5], differentiation [6][7][8], deposition of new structural proteins [9,10] and ultimately the regeneration of functional tissue [4,11,12]. Traditionally, methods such as NMR, FTIR and X-Ray spectroscopy have been used to assess biomaterials at the molecular level [13][14][15][16][17], while SEM and TEM have been invaluable tools for characterizing the three dimensional morphology of biomaterial scaffolds [5,[18][19][20][21][22][23]. Histology and immunostaining as well as assays for determining the expression levels of specific proteins are often used to assess how scaffolds interact with cells as tissues develop [5,10,18,20,[22][23][24].…”

Section: Competing Interest Statementmentioning

confidence: 99%

“…Traditionally, methods such as NMR, FTIR and X-Ray spectroscopy have been used to assess biomaterials at the molecular level [13][14][15][16][17], while SEM and TEM have been invaluable tools for characterizing the three dimensional morphology of biomaterial scaffolds [5,[18][19][20][21][22][23]. Histology and immunostaining as well as assays for determining the expression levels of specific proteins are often used to assess how scaffolds interact with cells as tissues develop [5,10,18,20,[22][23][24]. While all of these approaches provide sensitive and specific data, they are invasive.…”

Section: Competing Interest Statementmentioning

confidence: 99%

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Non-invasive characterization of structure and morphology of silk fibroin biomaterials using non-linear microscopy

et al. 2008

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Designing biomaterial scaffolds remains a major challenge in tissue engineering. Key to this challenge is improved understanding of the relationships between the scaffold properties and its degradation kinetics, as well as the cell interactions and the promotion of new matrix deposition. Here we present the use of non-linear spectroscopic imaging as a non-invasive method to characterize not only morphological, but also structural aspects of silkworm silk fibroin-based biomaterials, relying entirely on endogenous optical contrast. We demonstrate that two photon excited fluorescence and second harmonic generation are sensitive to the hydration, overall β sheet content and molecular orientation of the sample. Thus, the functional content and high resolution afforded by these noninvasive approaches offer promise for identifying important connections between biomaterial design and functional engineered tissue development. The strategies described also have broader implications for understanding and tracking the remodeling of degradable biomaterials under dynamic conditions both in vitro and in vivo.

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Porous Biomaterials

Annabi

2012

Integrated Biomaterials for Biomedical Technology

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Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cells

Cited by 293 publications

References 42 publications

Influence of flow rate and scaffold pore size on cell behavior during mechanical stimulation in a flow perfusion bioreactor

Influence of flow rate and scaffold pore size on cell behavior during mechanical stimulation in a flow perfusion bioreactor

Non-invasive characterization of structure and morphology of silk fibroin biomaterials using non-linear microscopy

Porous Biomaterials

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