Influence of Marolo (Annona crassiflora Mart.) Pulp Intake on the Modulation of Mutagenic/Antimutagenic Processes and Its Action on Oxidative Stress In Vivo

Abstract

Brazilian savanna constitutes a valuable ecoregion that contributes to the supply of fruit known worldwide for its nutritional value and peculiar flavors. Among them, the Marolo (Annona crassiflora Mart.) fruit is known for its use in folk medicine. In order to establish the safety of Marolo for human consumption, this study evaluated the following: the chemical composition of Marolo pulp; its mutagenic and antimutagenic activities using micronucleus test; and the oxidative stress induced in the livers of mice fed a diet containing 1%, 10% or 20% pulp. It was observed that the chemical composition of marolo pulp was similar to that of common fruit; nevertheless, its lipidic content and energetic values were higher. In the mice fed experimental diets, the biochemical parameters of the blood serum showed normal levels of glucose, triglycerides, and cholesterol. The micronucleus test indicated neither mutagenic nor antimutagenic effects of Marolo consumption on bone marrow cells but showed potentialization of cyclophosphamide (CP). The oxidative stress levels observed indicated that CP was not exerting a great influence on the induction of reactive oxygen species. As the whole fruit is a complex matrix, the interactions between its components could be responsible for its negative and positive biological effects.

Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis

“…Fat content was measured in a Soxhlet system by solvent extraction (ASSOCIATION OF OFFICIAL ANALYT ICAL CHE MIST S-AOAC, 2000) an d carbohydrates were calculated to the remainder (the difference using the fresh weight-derived). Details regarding the chemistry profile of the pulp used in this study have previously been described in Dragano et al (2010).…”

“…In vitro and in vivo studies indicate that either pulp or ethanolic extracts of A. crassiflora Mart.o leaves did not present potential mutagenic or genotoxic effects, as evaluated using micronuclei, SOS-Inductest or Ames tests (DRAGANO et al, 2010;FERRI;CHEN-CHEN, 2011). Although A. crassiflora Mart.…”

“…pulp, peel and seeds demonstrated antioxidant activity in vitro (ROESLER et al, 2007), Vilar et al (2008) showed both antimutagenic and cytotoxic effects of an ethanolic extract of marolo leaves on bone marrow cells. Also, Dragano et al (2010) showed that diets supplemented with 10% and 20% (w/w) A. crassiflora Mart. pulp acted as a cyclophosphamide booster in the same mouse bone marrow micronucleus test.…”

“…The pulp of the A. crassiflora Mart. contains carotenoids, (DRAGANO et al, 2010;ROESLER et al, 2007). Given their selective effects, some of these compounds are potential anti-cancer agents against different human cancer cell lines in vitro (JASWIR et al, 2012).…”

Marolo (Annona crassiflora Mart.): a study of value chain and processing

“…Among those plant species, Marolo (Annona crassiflora Mart. ), also known as Araticum, a fruit found in the Brazilian savanna, has a wide variety of potential use in spite of being limited to the consumption in natura by local population or mainly used in the preparation of juices, ice-creams, jellies, and jams (DRAGANO et al, 2010;ROESLER et al, 2007).…”