Influence of Material Composition on Structure, Surface Properties and Biological Activity of Nanocrystalline Coatings Based on Cu and Ti

Wojcieszak, Damian; Osękowska, Małgorzata; Kaczmarek, Danuta; Szponar, Bogumiła; Mazur, М.; Mazur, Piotr; Obstarczyk, Agata

doi:10.3390/coatings10040343

Cited by 8 publications

(18 citation statements)

References 88 publications

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“…The released ions potential toxic effect was evaluated in terms of metabolic activity by means of the alamar blue assay as previously detailed. Moreover, ions internalization and accumulation were histologically evaluated by using the May-Grünwald-Giemsa staining as described in previous literature [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Generation of cytocompatible superhydrophobic Zr–Cu–Ag metallic glass coatings with antifouling properties for medical textiles

Sharifikolouei

Najmi

Cochis

et al. 2021

Materials Today Bio

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Zirconium–Copper-based metallic glass thin films represent promising coatings in the biomedical sector for their combination of antibacterial property and wear resistance. However, finding a Zr–Cu metallic glass composition with desirable cytocompatibility and antibacterial property is extremely challenging. In this work, we have created a cytocompatible and (super-)hydrophobic Zr–Cu–Ag metallic glass coating with ≈95% antifouling properties. First, a range of different chemical compositions were prepared via Physical Vapor Deposition magnetron by co-sputtering Zr, Cu, and Ag onto a Polybutylene terephthalate (PBT) substrate among which Zr 93·5 Cu 6·2 Ag 0.2 , Zr 76·7 Cu 22·7 Ag 0.5, and Zr 69·3 Cu 30·1 Ag 0.6 were selected to be further investigate for their surface properties, antibacterial activity, and cytocompatibility. Scanning electron microscopy (SEM) images revealed a micro-roughness fibrous structure holding superhydrophobic properties demonstrated by specimens' static and dynamic contact angle measurements ranging from 130° to 150°. The dynamic contact angle measurements have shown hysteresis below 10° for all coated samples which indicated the superhydrophobicity of the samples. To distinguish between antifouling and bactericidal effect of the coating, ions release from coatings into Luria Bertani Broth (LB), and Dulbecco's Modified Eagle Medium (DMEM) solutions were evaluated by inductively coupled plasma mass spectrometry (ICP-MS) measurements after 24 h and 5 days. Antifouling properties were evaluated by infecting the specimens' surface with the Gram-positive Staphylococcus aureus and the Gram-negative Escherichia coli strain reporting a ≈95% reduction of bacteria adhesion as visually confirmed by FESEM and fluorescent live/dead staining. Human mesenchymal stem cells (hMSC) were used for direct cytocompatibility evaluation of coated samples and their metabolic activity was evaluated via relative fluorescence unit after 24 h and 5 days confirming that it was comparable to the controls (>97% viable cells). The results were further visualized by FESEM, fluorescent staining by Live/Dead Viability/Cytotoxicity Kit and confirmed the cytocompatibility of all coated samples. Finally, hMSC′ cytoplasm was stained by May Grunwald and Giemsa after 5days to detect and visualize the released ions which have diffused through the cells' membrane.

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Section: Methodsmentioning

confidence: 99%

Generation of cytocompatible superhydrophobic Zr–Cu–Ag metallic glass coatings with antifouling properties for medical textiles

Sharifikolouei

Najmi

Cochis

et al. 2021

Materials Today Bio

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“…Wojcieszak et al [ 116 ] prepared Cu-doped nanocrystalline coatings ( Figure 8 ) using the pulsed DC magnetron sputtering technique. There was a significant change in the structure of Cu-Ti films with the increase of Cu content.…”

Section: Preparation Techniques and Related Properties Of Cu-doped Ti...mentioning

confidence: 99%

Recent Advances in Copper-Doped Titanium Implants

Zhou

Zeng

et al. 2022

Materials

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Titanium (Ti) and its alloys have been extensively used as implant materials in clinical practice due to their high corrosion resistance, light weight and excellent biocompatibility. However, the insufficient intrinsic osteogenic capacity of Ti and its alloys impedes bone repair and regeneration, and implant-related infection or inflammation remains the leading cause of implant failure. Bacterial infections or inflammatory diseases constitute severe threats to human health. The physicochemical properties of the material are critical to the success of clinical procedures, and the doping of Cu into Ti implants has been confirmed to be capable of enhancing the bone repair/regeneration, angiogenesis and antibacterial capability. This review outlines the recent advances in the design and preparation of Cu-doped Ti and Ti alloy implants, with a special focus on various methods, including plasma immersion implantation, magnetron sputtering, galvanic deposition, microarc oxidation and sol-gel synthesis. More importantly, the antibacterial and mechanical properties as well as the corrosion resistance and biocompatibility of Cu-doped Ti implants from different methods are systematically reviewed, and their prospects and limitations are also discussed.

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“…100 nm in length, without empty spaces between the densely packed grains. The hydrophilic nature of the surface promotes cell adhesion [15,18]. Table 1.…”

Section: Physicochemical Properties Of Thin Films Based On Cu and Timentioning

confidence: 99%

“…Research on nanocrystalline copper-and titanium-based materials continues towards finding a compound with an optimal ratio of the diverse properties of Cu and Ti that is safe for eukaryotic cells while still significantly reducing the number of microorganisms. We have tested a series of thin nanocrystalline coatings based on copper and titanium in different material compositions [10,[15][16][17]. Recent studies have characterized the physicochemical properties of Cu-Ti thin films with different material compositions [18], as well as the preliminary results concerning their cytotoxicity [15].…”

Section: Introductionmentioning

confidence: 99%

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Multifunctional Nanocrystalline Cu–Ti Thin Films Enhance Survival and Induce Proliferation of Mouse Fibroblasts In Vitro

et al. 2021

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This paper describes the effect of a nanocrystalline thin film based on copper and titanium on mouse fibroblast cells. Cu–Ti coatings were prepared using magnetron sputtering. In their composition was 25 at.% Cu and 75 at.% Ti. The goal of the study was to evaluate the effect of the material on the survival, migration, and proliferative capabilities of mouse L929 fibroblasts. The Cu25Ti75 material had no effect on the induction of cell death and did not disturb the cell cycle phase. The study showed a unique effect of a Cu25Ti75 thin film on mouse fibroblast cells, and the results concerning mitochondrial activity, cell proliferation, and migration proved that the material is nontoxic and shows proliferative properties in a wound healing test. The possible biomedical applications of the new nanocrystalline thin film biomaterial with multifunctional properties are described.

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Influence of Material Composition on Structure, Surface Properties and Biological Activity of Nanocrystalline Coatings Based on Cu and Ti

Cited by 8 publications

References 88 publications

Generation of cytocompatible superhydrophobic Zr–Cu–Ag metallic glass coatings with antifouling properties for medical textiles

Generation of cytocompatible superhydrophobic Zr–Cu–Ag metallic glass coatings with antifouling properties for medical textiles

Recent Advances in Copper-Doped Titanium Implants

Multifunctional Nanocrystalline Cu–Ti Thin Films Enhance Survival and Induce Proliferation of Mouse Fibroblasts In Vitro

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