Influence of Maternal and Larval Immunisation against <i>Lactococcus garviae</i> Infection in Rainbow Trout <i>Oncorhynchus mykiss</i> (Walaum) Lysozyme Activity and IgM Level

Abstract

How to cite this paper: Akbary, P., Mirvaghefi, A.R., Akhlaghi, M. and Fereidouni, M.S. (2015) AbstractThis study evaluated efficacy of maternal and larval immunisation against Lactococcus garviae infection and on the lysozyme and immunoglobulin (IgM) levels in rainbow trout Oncorhynchus mykiss (Walaum). Forty-eight-day-old larvae (mean weight 96 mg) originating from injected weekly with letrozole and immunised, only immunised and non-immunised parents were experimentally infected with the L. garvieae, and the mortality rate was recorded daily. Larvae were vaccinated by immersion at 58 days post hatch with live L. garvieae (10 9 cells/mL) for 15 min. Every third day post larvae vaccination, two larvae from each group were collected for analysis lysozyme (by a method based on the ability of lysozyme to lyse the bacterium Micrococcus lysodeikticus) and IgM (by enzyme-linked immunosorbent assay (ELISA)) parameters. Vaccinated and control larvae were tested for protection against L. garvieae 30 days post larvae immunization when the larvae were 88 days old. Larvae were challenged by bath exposure with live L. garvieae (10 9 cells/mL) for 2 min and monitored for mortality for at least 10 days following challenge. The challenge experiment with L. garvieae showed a significant reduction in larvae from immunised (54.44% ± 0.64%) and injected weekly with letrozole and immunised fish (52.96% ± 0.97%) compared to larvae from control fish (62.96% ± 2.22%). Vaccinated larvae originated from injected weekly with letrozole and immunised parents showed significantly higher lysozyme activity compared to other fish groups. Vaccinated larvae showed significantly less mortality compared to controls. The rela-* Corresponding author.

Maternal Immunity Transfer of Zebrafish (Danio rerio) following Vaccination with a Live-Attenuated Vibrio harveyi Vaccine

“…Fish larvae depend highly on their maternalderived immune materials and nutrient composition for their development. Akbary et al [38] highlighted the high mortality in fsh fry recorded globally due to outbreaks of microbial pathogens, hence the importance of maternally derived immunity for the survival of ofspring in the early stage before hatching [19].…”