Influence of media composition on xylitol fermentation by Candida guiliiermondii using response surface methodology

Roberto, Inês Conceição; Sato, Sunao; Mancilha, Ismael Maciel de; Taqueda, Maria Elena Santos

doi:10.1007/bf00128390

Cited by 50 publications

(25 citation statements)

References 11 publications

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“…Conversely, xylitol seems to be solely produced from xylose metabolism. Polyols production in non-supplemented BSG hydrolysates suggested that some essential nutrients are already present, as previously described for rice straw hydrolysate [35]. Ethanol and glycerol were also produced, but only in small amounts.…”

Section: Effect Of Hydrolysates Supplementation On Xylitol Productionmentioning

confidence: 63%

Supplementation requirements of brewery’s spent grain hydrolysate for biomass and xylitol production by Debaryomyces hansenii CCMI 941

Carvalheiro

Duarte

Lopes

et al. 2006

J IND MICROBIOL BIOTECHNOL

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The effect of nutrient supplementation of brewery's spent grain (BSG) hydrolysates was evaluated with respect to biomass and xylitol production by Debaryomyces hansenii. For optimal biomass production, supplementation of full-strength BSG hydrolysates required only phosphate (0.5 g l(-1) KH(2)PO(4)), leading to a biomass yield and productivity of 0.60 g g(-1) monosaccharides and 0.55 g l(-1 )h(-1), respectively. Under the conditions studied, no metabolic products other than CO(2) and biomass were identified. For xylitol production, fourfold and sixfold concentrated hydrolysate-based media were used to assess the supplementation effects. The type of nutrient supplementation modulated the ratio of total polyols/total extracellular metabolites as well as the xylitol/arabitol ratio. While the former varied from 0.8 to 1, the xylitol/arabitol ratio reached a maximum value of 2.6 for yeast extract (YE)-supplemented hydrolysates. The increase in xylitol productivity and yield was related to the increase of the percentage of consumed xylose induced by supplementation. The best xylitol yield and productivity were found for YE supplementation corresponding to 0.55 g g(-1) and 0.36 g l(-1 )h(-1), respectively. In sixfold concentrated hydrolysates, providing that the hydrolysate was supplemented, the levels of xylitol produced were similar or higher than those for arabitol. Xylitol yield exhibited a further increase in the sixfold hydrolysate supplemented with trace elements, vitamins and minerals to 0.65 g g(-1), albeit the xylitol productivity was somewhat lower. The effect of using activated charcoal detoxification in non-supplemented versus supplemented sixfold hydrolysates was also studied. Detoxification did not improve polyols formation, suggesting that the hemicellulose-derived inhibitor levels present in concentrated BSG hydrolysates are well tolerated by D. hansenii.

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Section: Effect Of Hydrolysates Supplementation On Xylitol Productionmentioning

confidence: 63%

Supplementation requirements of brewery’s spent grain hydrolysate for biomass and xylitol production by Debaryomyces hansenii CCMI 941

Carvalheiro

Duarte

Lopes

et al. 2006

J IND MICROBIOL BIOTECHNOL

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“…Considering the important effects and interaction of time and temperature, a new study was conducted including a new variable (substrate concentration) to increase this concentration. The influence of substrate concentration, temperature and time during the enzymatic hydrolysis of potato was assessed using the response surface methodology which has been successfully used to optimize biochemistry and biotechnology processes related with food systems (Larrazá bal & Camacho, 2008;Roberto, Sato, Mancilha, & Tacheda, 1995;Vá zquez & Martin, 1998).…”

Section: Response Surface Of the Dependence Of Reducing Sugar Concentmentioning

confidence: 99%

A kinetic assessment of the enzymatic hydrolysis of potato (Solanum tuberosum)

Delgado

Castro

Vázquez

2009

LWT - Food Science and Technology

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“…Experimental designs have been extensively used for optimization of fermentative processes due to the possibility of simultaneously evaluating several factors, as well as verifying the individual and combined effects of each variable [15]. In the present study, an experimental design was thus used to identify and quantify the inhibitory effects of the variables acetic acid, ferulic acid, and syringaldehyde on xylitol production by Candida guilliermondii yeast.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory action of toxic compounds present in lignocellulosic hydrolysates on xylose to xylitol bioconversion by Candida guilliermondii

Pereira¹,

Mussatto²,

Roberto³

2010

J Ind Microbiol Biotechnol

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The inhibitory action of acetic acid, ferulic acid, and syringaldehyde on metabolism of Candida guilliermondii yeast during xylose to xylitol bioconversion was evaluated. Assays were performed in buffered and nonbuffered semidefined medium containing xylose as main sugar (80.0 g/l), supplemented or not with acetic acid (0.8-2.6 g/l), ferulic acid (0.2-0.6 g/l), and/or syringaldehyde (0.3-0.8 g/l), according to a 2(3) full factorial design. Since only individual effects of the variables were observed, assays were performed in a next step in semidefined medium containing different concentrations of each toxic compound individually, for better understanding of their maximum concentration that can be present in the fermentation medium without affecting yeast metabolism. It was concluded that acetic acid, ferulic acid, and syringaldehyde are compounds that may affect Candida guilliermondii metabolism (mainly cell growth) during bioconversion of xylose to xylitol. Such results are of interest and reveal that complete removal of toxic compounds from the fermentation medium is not necessary to obtain efficient conversion of xylose to xylitol by Candida guilliermondii. Fermentation in buffered medium was also considered as an alternative to overcome the inhibition caused by these toxic compounds, mainly by acetic acid.

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Influence of media composition on xylitol fermentation by Candida guiliiermondii using response surface methodology

Cited by 50 publications

References 11 publications

Supplementation requirements of brewery’s spent grain hydrolysate for biomass and xylitol production by Debaryomyces hansenii CCMI 941

Supplementation requirements of brewery’s spent grain hydrolysate for biomass and xylitol production by Debaryomyces hansenii CCMI 941

A kinetic assessment of the enzymatic hydrolysis of potato (Solanum tuberosum)

Inhibitory action of toxic compounds present in lignocellulosic hydrolysates on xylose to xylitol bioconversion by Candida guilliermondii

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