Influence of Micro-/Nano-Particles on Osteoblast-Like Cells: A Static and Time Lapse Observation

Abstract

Cytotoxicity and cell behavior to micro / nanoparticles of TiO2 and CuO was evaluated using the viability measurement and time-lapse observation. After cultured, osteoblastic cells MC3T3-E1 were exposed to particles. After 24 hour exposure, their morphology was observed using a SEM and the viability was measured. Cells exposed to TiO2 indicated no or very low decrease of viability. The results were independent of the particle size. On the other hand, the viability of cells exposed to CuO decreased with the concentration, and showed the size dependence. The nanosized CuO indicated higher toxicity compared with micro-sized one. Dynamic behavior of cells exposed to nanoparticles, was succeeded to observe in a time-lapse method for 24 hours. The observation showed that the cells exposed to CuO became dead after forming a spherical shape. This is consistent with the image taken by SEM. Time-lapse observation made it possible to see the dynamic reaction process from cell contact to particles at first, the following cell activity response and finally to cell death, which revealed a considerably different morphology from the static cell observed after fixation by conventional method.

Preparation and biocompatibilities of luminescent europium-doped yttria and titania nanoparticles

“…These phenomena were also observed when mouse osteoblast-like cells (MC3T3-E1) were exposed to similar nanoparticles (TiO 2 , In 2 O 3 , and CuO). 24), 31) This tendency was in good agreement with the data from nanoparticle exposure to fibroblast cells. 32) When the cells were incubated for 3 days with TiO 2 nanoparticles, they exhibited good viability even when the concentration was increased to 30 ppm.…”

“…0.1 and 0.3¯m, respectively (Table 1). 24) Figure 4 shows a typical emission and excitation spectra of the Eu-doped particles. A strong sharp emission line appeared at 611 nm with 390 nm excitation.…”

Biocompatibility and Biodistribution of Several Nano-Sized Ceramics Particles

“…When the cell was in contact with particles, it caused cell death. However, cells that did not come into contact with the particle remained the moving actively [13]. In addition, we also exposed copper ion to the cells for understanding the reason of the inflammation.…”

Cytocompatiblity of Ceramic Nanoparticles to Various Types of Cells

“…The luminescence signals were quantified using a luminometer (GloMax96 Microplate Luminometer, Promega). These procedures are described in detail elsewhere [10].…”