Influence of mineral amendment on disease suppressive activity of Pseudomonas fluorescens to Fusarium wilt of chickpea

Saikia, Ratul; Varghese, Saju; Arora, Dilip K.

doi:10.1016/j.micres.2007.05.001

Cited by 29 publications

(22 citation statements)

References 26 publications

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“…Hence, fish culture practices may have profound influences on bacterial distributions. This may, in fact, be beneficial to the eggs because pseudomonads are often referred to in agriculture as important members of the growth-promoting rhizobacteria that induce systemic resistance in plants against certain pests and diseases (Ramamoorthy et al 2001;Saikia et al 2003Saikia et al , 2009). Preparations of P. fluorescens can even have antagonistic activities against fish pathogens such as Vibrio anguillarum (Gram et al 1999) and Aeromonas salmonicida (Gram et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Bacterial Analysis of Fertilized Eggs of Atlantic Salmon from the Penobscot, Naraguagus, and Machias Rivers, Maine

Cipriano

2015

J Aqua Anim Hlth

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Serious losses have occurred at the U.S. Fish and Wildlife Service, Craig Brook National Fish Hatchery, East Orland, Maine, among eggs that were taken from Atlantic Salmon Salmo salar, which were held as captive broodfish during their returns to the Penobscot River, Naraguagus River, and Machias River to spawn. Bacterial isolations were attempted from external surfaces and the internal contents of individual eggs. Externally and in all cases, Pseudomonas fluorescens was the predominant bacterium associated with the surface of all eggs. These bacteria were resistant to a surface treatment of 1,667 ppm formalin for 15 min and, therefore, the monoclonal nature of P. fluorescens on egg surfaces was considered to result from its ability to resist the germicidal activity of formalin administered for antifungal treatments. Flavobacterium psychrophilum, the cause of bacterial coldwater disease, was isolated from the interior of 23.6, 18.1, and 29.2% of the dead Atlantic Salmon eggs from Penobscot River egg lots A-98, A-100, and A-101, respectively, and concentrations of this pathogen ranged from 1.0 × 10(3) to >5 × 10(8) CFU per gram of dead egg. Flavobacterium psychrophilum was also isolated from 8.3, 26.7, and 10.0% of the dead eggs from Naraguagus River egg lots N-158, N-161, and N-163, respectively, in which concentrations of this organism ranged from 1.0 × 10(3) to 7.5 × 10(8) CFU per gram of egg. This bacterium was also isolated from within 18.3% and 3.3% of the dead eggs from Machias River egg lots M-128 and M-142, respectively, and its concentrations ranged from 1.0 × 10(3) to 1.5 × 10(8) CFU per gram of egg. The finding of F. psychrophilum from within these eggs is indicative of this pathogen's widespread and persistent prevalence in Atlantic Salmon in New England.

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Section: Discussionmentioning

confidence: 99%

Bacterial Analysis of Fertilized Eggs of Atlantic Salmon from the Penobscot, Naraguagus, and Machias Rivers, Maine

Cipriano

2015

J Aqua Anim Hlth

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“…radicislycopersici acts as a chemical signal that represses DAPG production by P. protegens CHA0 (28). Since that discovery was reported, others have substantiated that FA represses the production of DAPG (31,38) and phenazines (39) and alters the production of pyoverdine siderophores (40,41) by strains of Pseudomonas spp. FA acts, directly or indirectly, by influencing the expression of biosynthesis genes, including genes for the biosynthesis of DAPG (42)(43)(44) and phenazine-1-carboxylate (39).…”

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confidence: 94%

An Interspecies Signaling System Mediated by Fusaric Acid Has Parallel Effects on Antifungal Metabolite Production by Pseudomonas protegens Strain Pf-5 and Antibiosis of Fusarium spp

Quecine

Kidarsa

Goebel

et al. 2016

Appl Environ Microbiol

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dPseudomonas protegens strain Pf-5 is a rhizosphere bacterium that suppresses soilborne plant diseases and produces at least seven different secondary metabolites with antifungal properties. We derived mutants of Pf-5 with single and multiple mutations in biosynthesis genes for seven antifungal metabolites: 2,4-diacetylphoroglucinol (DAPG), pyrrolnitrin, pyoluteorin, hydrogen cyanide, rhizoxin, orfamide A, and toxoflavin. These mutants were tested for inhibition of the pathogens Fusarium verticillioides and Fusarium oxysporum f. sp. pisi. Rhizoxin, pyrrolnitrin, and DAPG were found to be primarily responsible for fungal antagonism by Pf-5. Previously, other workers showed that the mycotoxin fusaric acid, which is produced by many Fusarium species, including F. verticillioides, inhibited the production of DAPG by Pseudomonas spp. In this study, amendment of culture media with fusaric acid decreased DAPG production, increased pyoluteorin production, and had no consistent influence on pyrrolnitrin or orfamide A production by Pf-5. Fusaric acid also altered the transcription of biosynthetic genes, indicating that the mycotoxin influenced antibiotic production by Pf-5 at the transcriptional level. Addition of fusaric acid to the culture medium reduced antibiosis of F. verticillioides by Pf-5 and derivative strains that produce DAPG but had no effect on antibiosis by Pf-5 derivatives that suppressed F. verticillioides due to pyrrolnitrin or rhizoxin production. Our results demonstrated the importance of three compounds, rhizoxin, pyrrolnitrin, and DAPG, in suppression of Fusarium spp. by Pf-5 and confirmed that an interspecies signaling system mediated by fusaric acid had parallel effects on antifungal metabolite production and antibiosis by the bacterial biological control organism. Pseudomonas is a heterogeneous genus of Gammaproteobacteria composed of species with diverse ecological roles; certain strains are members of the plant microbiome contributing to plant growth and health. Our studies focus on the soil bacterium Pseudomonas protegens Pf-5, which is known for its capacity to suppress plant diseases and produce a large spectrum of metabolites with antibiotic activity (1, 2). The antibiotics produced by Pf-5 include pyrrolnitrin (3), pyoluteorin (4), analogs of rhizoxin (5, 6), hydrogen cyanide (7), 2,4-diacetylphloroglucinol (DAPG) (8), monoacetylphloroglucinol (MAPG) (9) (an intermediate in the DAPG biosynthetic pathway [10,11]), the lipopeptide orfamide A (12), and toxoflavin (13). The antibiotics produced by Pf-5 have distinct but overlapping activity spectra, and it is likely that multiple compounds contribute to the interactions of Pf-5 with target plant pathogens that result in disease suppression. Commonly, the role of an antibiotic in biological control is assessed by comparing the level of disease suppression provided by a wild-type strain versus an antibiotic-deficient mutant (14). For strain Pf-5, however, this approach has not been highly successful in providing evidence for the roles of antibioti...

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“…p isi (Landa et al, 2002), Fo f. sp. ciceri (Saikia et al, 2009), Fo f. sp. lycopersici (Manikamdan et al, 2010) and Fo f. sp.…”

Section: Introductionmentioning

confidence: 99%

The effect of Pseudomonas chlororaphis subsp. aurantiaca strain Q16 able to inhibit Fusarium oxysporum growth on potato yield

Poštić¹,

Jošić²,

Lepsanovic³

et al. 2019

Ratar i povrt

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This study assesses the potential of antibiotics-producing Pseudomonas chlororaphis strains to increase potato yield and to inhibit the mycelial growth of phytopathogenic fungi Fusarium oxysporum (Fo) isolated from potato. P. chlororaphis subsp. aurantiaca strain Q16 (PchlQ16) caused the highest growth inhibition (67.07%) of FoA2 isolate in vitro. In field trials the effect of PchlQ16 was measured as the number of stems, number and weight of tubers and a total potato yield of the Rudolph potato variety. Application of P. chlororaphis and the number of treatments exhibited a significant effect on the yield. Two treatments of PchlQ16 increased the total yield of tubers from 4.9% to 33.05%, while four treatments from 9.3% to 92.35%, compared to the control. Based on our field results we can recommend a frequent application of PchlQ16 (4 times) during potato growth season. The results of our in vitro experiment support these findings as the bacterial strain suppressed growth of F. oxysporum. In this investigation PchlQ16 was confirmed as an effective growth promoting agent in potato production and can be highly effective in prevention of F. oxysporum infection.

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Influence of mineral amendment on disease suppressive activity of Pseudomonas fluorescens to Fusarium wilt of chickpea

Cited by 29 publications

References 26 publications

Bacterial Analysis of Fertilized Eggs of Atlantic Salmon from the Penobscot, Naraguagus, and Machias Rivers, Maine

Bacterial Analysis of Fertilized Eggs of Atlantic Salmon from the Penobscot, Naraguagus, and Machias Rivers, Maine

An Interspecies Signaling System Mediated by Fusaric Acid Has Parallel Effects on Antifungal Metabolite Production by Pseudomonas protegens Strain Pf-5 and Antibiosis of Fusarium spp

The effect of Pseudomonas chlororaphis subsp. aurantiaca strain Q16 able to inhibit Fusarium oxysporum growth on potato yield

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