Influence of n-Hexadecane and Naphthalene on Anaerobic Digestion: Kinetic Simulation, DOM Variation and Microbial Community Assessment

Abstract

The influences of n-Hexadecane and naphthalene on anaerobic digestion in batch tests were investigated. The batch kinetic analysis showed that the maximum methane accumulations (141.18 mL of n-Hexadecane and 146.76 mL of naphthalene) and production rates (20.48 mL/h of n-Hexadecane and 20.88 mL/h of naphthalene) were obtained while adding with 30 mg/L n-Hexadecane and 2 mg/L naphthalene, respectively. However, a significant inhibition ratio of 10% was observed at 100 mg/L of n-Hexadecane and 20 mg/L of naphthalene, respectively. The 3D-EEM results showed that the naphthalene was degraded by related microorganism as potential carbon source with concentration lower than 10 mg/L. In addition, the microbial community analysis indicated that the abundance of hydrotrophic Methanolinea and Methanobacterium increased from 7.92% to 12.76% as the increase n-Hexadecane, while decreased from 13.01% to 9.07% with increase of naphthalene. Contrastly, the acetoclasstic methanogens were increased due to the well synthetic action with syntrophic VFA-oxidizing bacteria.

Anthracene and Pyrene Biodegradation Performance of Marine Sponge Symbiont Bacteria Consortium

“…The number of new peaks formed tended to increase with interaction time. These new peaks had different retention times and peak heights, indicating different types and abundances [1,4,53]. Each new component had different characteristics, i.e., functional groups, but they were all organic compounds.…”

“…These PAHs are also present in plants (e.g., tobacco tar) and animals (e.g., deer and termites) [2]. Anthracene is moderately toxic and carcinogenic, whereas pyrene is highly toxic, carcinogenic, and genotoxic or mutagenic [3][4][5]. Anthracene is widely used as an antiseptic and insecticide, whereas pyrene is used for increasing the octane number of fossil fuels [6,7].…”

Comparison of Biodegradation Performance of Marine Sponge Symbiont Bacteria Consortium Against Anthracene and Pyrene

“…The number of new peaks formed is relatively more with increasing interaction time. These new peaks have different retention times, peak heights vary, indicating different types and abundances of these components [1,4,53]. Each new component that appears has its characteristics, namely functional groups, but as a whole is a group of organic compounds.…”

“…Interaction between bacterial suspension and PAHs aerated on a shaker incubator at 200 rpm. The interaction time is 25 days, and every five days observations are made, biodegradation parameters are measured [4,11,58].…”

“…Anthracene is a moderately toxic and carcinogenic PAHs compound. Pyrene is a highly toxic PAHs group, carcinogenic and genotoxic or mutagenic [3][4][5]. Anthracene is widely used as an antiseptic and insecticide, while pyrene increases the octane number of fossil fuels [6,7].…”