Influence of needle gauge used for venipuncture on automated platelet count and coagulation profile in dogs

Greenwell, C. M.; Epstein, Steven E; Brain, Patrick

doi:10.1111/avj.12150

Cited by 8 publications

(8 citation statements)

References 17 publications

(28 reference statements)

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“…The pig industry was found to fall broadly into two categories: larger farms that were vertically integrated, purchased semen to introduce new genetic material and did not market through saleyards and smaller producers that regularly used saleyards to both purchase and sell pigs. This study compliments that written by Schembri et al, 5 published recently, who examined the demographics and practices of pig farmers trading at saleyards in eastern Australia. Although the two studies have different study populations, different aims and were conducted 6 years apart, where the results overlap the findings are consistent.…”

Section: Editorial Emergency Disease Planning and Preparednesssupporting

confidence: 71%

“…A prospective, observational, randomised clinical study included 20 clinically healthy dogs in which blood was collected from the jugular veins using 21G, 23G and 25G needles. 5 Results showed no difference in the automated platelet count or automated coagulation times for the different needle gauges used, suggesting that the results of venipuncture are not altered by needle size.…”

Section: Small Animalsmentioning

confidence: 86%

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In this issue – March 2014

Jackson¹

2014

Aust Veterinary J

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Section: Editorial Emergency Disease Planning and Preparednesssupporting

confidence: 71%

Section: Small Animalsmentioning

confidence: 86%

In this issue – March 2014

Jackson¹

2014

Aust Veterinary J

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“…To our knowledge, no studies in other species have found any difference in PT on blood drawn with different needle sizes, 6,7 and the finding that PT is longer with smaller gauge needles is unexpected. In theory, using a smaller-diameter needle requires a higher vacuum, which will exert higher shear forces on the blood, and more activation of platelets and coagulation factors, potentially leading to a shorter PT.…”

Section: Discussionmentioning

confidence: 73%

“…6 In dogs, no differences were found in those variables when comparing 21, 23 and 25 G needles. 7 The recommended needle gauge used for venipuncture in human medicine is 21 G, when possible. 6 There are no evidence-based recommendations in feline medicine, but there have been suggestions in the literature to use 21 G needles.…”

Section: Introductionmentioning

confidence: 99%

Influence of needle gauge used for venipuncture on measures of hemostasis in cats

Solbak

Epstein

Hopper

2018

Journal of Feline Medicine and Surgery

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Objectives The objective of this study was to evaluate the effect of different needle sizes used to obtain blood via jugular venipuncture in cats on routine measures of hemostasis. Methods This was a prospective, observational, randomized, clinical study carried out at a university teaching hospital. Twenty healthy, client-owned cats were used. Results Each cat had blood collected via direct venipuncture from both jugular veins. Sampling of the right and left jugular vein was randomized to be collected with either a 22 G or a 25 G needle, respectively, and routine coagulation variables and platelet count were performed on all samples. Values were analyzed for differences in needle size, and site of sample collection. There was no difference between the two needle gauges in activated partial thromboplastin time, platelet count, fibrinogen degradation products, or fibrinogen, or between sampling from the left and right jugular vein. Prothrombin time (PT) was significantly higher when drawn from a 25 G needle (11.7 s) compared with a 22 G needle (11.4 s) ( P = 0.01), but not different in left vs right jugular vein samples. Bland-Altman analysis of PT comparing for 25 G minus 22 G needle vs the average, calculated a mean bias (95% limits of agreement) of 0.49 s (-1.4 s to 2.4 s). Conclusions and relevance This study of 20 healthy cats found that the use of either a 25 G or 22 G needle for jugular venipuncture did not introduce any clinically meaningful difference in routine coagulation variables or platelet count.

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“…It is known that venepuncture with a higher gauge needle can reduce patient morbidity, by alleviating discomfort during blood collection and reducing the risk of subsequent haemorrhage. 16 Therefore, in feline patients, an ideal needle size should be selected for safer blood collection; this depends on factors such as patient size, patient cooperation and predisposition to haemorrhage.…”

Section: Discussionmentioning

confidence: 99%

Influence of needle gauge and venepuncture difficulty on thromboelastography in healthy cats

Shin

Nam

Song

et al. 2018

Journal of Feline Medicine and Surgery

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Objectives The aim of this study was to evaluate the effect of two differently sized butterfly catheter needles and the effect of venepuncture difficulty on thromboelastography (TEG) results in healthy cats. Methods Twenty-four healthy cats were included. Blood samples were collected from the jugular vein by syringe aspiration via direct venepuncture with 21 G and 22 G butterfly needles. The venepuncture difficulty score was classified into four categories. The first 1.5 ml blood drawn from each subject was discarded before collecting a sample for TEG analysis. TEG analyses were performed on citrated whole blood samples from 17 clinically healthy cats, using assays with kaolin as activators. Among the TEG parameters, reaction time (R), clot formation time (κ), alpha angle (α), maximum amplitude (MA) and global clot strength (G) were recorded from each tracing. Results Seven cats were excluded from the study; results were obtained for the remaining 17 cats. There were no statistically significant differences between the use of two different needles for R ( P = 0.72), κ ( P = 0.74), α ( P = 0.99), MA ( P = 0.08) and G ( P = 0.09). Samples with difficulty scores ⩾1 were not significantly different from samples with difficulty scores of 0 for R ( P = 0.24), κ ( P = 0.65), α ( P = 0.65), MA ( P = 0.72) and G ( P = 0.77). Conclusions and relevance The results of TEG in clinically healthy cats do not differ significantly when using two different gauge needles. There was no significant difference in the TEG results according to venepuncture difficulty scoring.

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Influence of needle gauge used for venipuncture on automated platelet count and coagulation profile in dogs

Abstract: Venipuncture can be performed with a 21G, 23G or 25G needle to obtain blood from dogs for automated platelet count and PT/aPTT measurement without affecting the results.

Cited by 8 publications

References 17 publications

In this issue – March 2014

In this issue – March 2014

Influence of needle gauge used for venipuncture on measures of hemostasis in cats

Influence of needle gauge and venepuncture difficulty on thromboelastography in healthy cats

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